Patents by Inventor Lifang Ruan

Lifang Ruan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Enhancin gene bel1 from and its use in biological insecticide
    Patent number: 8623651
    Abstract: The isolation, cloning of an enhancin gene bel1 from Bacillus thuringiensis, and the use of an enhancin Bel1 in biological insecticides are disclosed in the present invention. A new enhancin gene bel1 is isolated from Bacillus thuringiensis subsp. kurstaki YBT-1520, and its encoded product is a new enhancin Bel1. The enhancin has very strong effect-enhancing activity on the insecticidal action of insecticidal crystal protein Cry1Ac in killing Lepidoptera insects. The insecticidal activity of genetically engineered Bacillus thuringiensis BMB0187 developed on the basis of this enhancin is 5.7 times as high as that of the initial strain.
    Type: Grant
    Filed: November 23, 2009
    Date of Patent: January 7, 2014
    Assignee: Huazhong Agricultural University
    Inventors: Ming Sun, Ziniu Yu, Shangling Fang, Donghai Peng, Lifang Ruan, Shouwen Chen, Chunping Luo
  • ENHANCIN GENE BEL1 FROM BACILLUS THURINGIENSIS AND ITS USE IN BIOLOGICAL INSECTICIDE
    Publication number: 20120156167
    Abstract: The isolation, cloning of an enhancin gene bel1 from Bacillus thuringiensis, and the use of an enhancin Bel1 in biological insecticides are disclosed in the present invention. A new enhancin gene bel1 is isolated from Bacillus thuringiensis subsp. kurstaki YBT-1520, and its encoded product is a new enhancin Bel1. The enhancin has very strong effect-enhancing activity on the insecticidal action of insecticidal crystal protein Cry1Ac in killing Lepidoptera insects. The insecticidal activity of genetically engineered Bacillus thuringiensis BMB0187 developed on the basis of this enhancin is 5.7 times as high as that of the initial strain.
    Type: Application
    Filed: November 23, 2009
    Publication date: June 21, 2012
    Applicant: HUAZHONG AGRICULTURAL UNIVERSITY
    Inventors: Ming Sun, Ziniu Yu, Shangling Fang, Donghai Peng, Lifang Ruan, Shouwen Chen, Chunping Luo
  • GENE CLUSTER FOR THURINGIENSIN SYNTHESIS
    Publication number: 20120015404
    Abstract: The invention belongs to the technical field of genetic engineering of agricultural microorganisms. Specifically, the invention relates to producing and using gene cluster for thuringiensin synthesis from Bacillus thuringiensis. The gene cluster includes 11 genes of thuA, thuB, thuC, thuD, thuE, thuF, thuG, thu1, thu2, thu3 and thu4. These genes have the nucleotide sequence as shown in SEQ ID NO: 1. And the thuringiensin biosynthetase encoded by these genes have the amino acid sequences as shown in SEQ ID NO: 2-12. The genes according to the invention and the proteins encoded by those genes can be used as new nucleosides lead compound of pesticide library and provide new target for developing insecticides. In practical use, a series of new, efficient and low toxic insecticide can be obtained by structure modification.
    Type: Application
    Filed: December 15, 2009
    Publication date: January 19, 2012
    Applicant: HUAZHONG AGRICULTURAL UNIVERSITY
    Inventors: Ming Sun, Ziniu Yu, Lifang Ruan, Shouwen Chen, Xiaoyan Liu, Donghai Peng
  • Gene encoding an insecticidal crystal protein of
    Patent number: 7838293
    Abstract: The present invention discloses the isolation, cloning and use of insecticidal crystal proteins from Bacillus thuringiensis. The present invention isolates a novel insecticidal crystal protein gene cry7Ba1 from B. thuringiensis subsp. huazhongensis YBT-978 strain, and said gene encodes a novel insecticidal crystal protein Cry7Ba1, which shows insecticidal activity against Lepidopteran insects. The present invention also discloses the gene sequence of the novel insecticidal crystal protein, uses suitable expression vectors to transform micro-organisms so as to express the product Cry7Ba1 encoded by the gene and make it exert the insecticidal activity of the protein against Lepidopteran pests.
    Type: Grant
    Filed: October 17, 2006
    Date of Patent: November 23, 2010
    Assignee: Huazhong Agricultural University
    Inventors: Ming Sun, Ziniu Yu, Shouwen Chen, Zhenyu Zhang, Lifang Ruan, Suxia Guo, Jingyuan Dai, Lin Li, Ziduo Liu
  • Gene Cry7ba1 Encoding and Insecticidal Crystal Protein of Bacillus Thuringiensis
    Publication number: 20090130758
    Abstract: The present invention discloses the isolation, cloning and use of insecticidal crystal proteins from Bacillus thuringiensis. The present invention isolates a novel insecticidal crystal protein gene cry7Bal from B. thuringiensis subsp. huazhongensis YBT-978 strain, and said gene encodes a novel insecticidal crystal protein Cry7Bal, which shows insecticidal activity against Lepidopteran insects. The present invention also discloses the gene sequence of the novel insecticidal crystal protein, uses suitable expression vectors to transform micro-organisms so as to express the product Cry7Bal encoded by the gene and make it exert the insecticidal activity of the protein against Lepidopteran pests.
    Type: Application
    Filed: October 17, 2006
    Publication date: May 21, 2009
    Applicant: HUAZHONG AGRICULTURAL UNIVERSITY
    Inventors: Ming Sun, Ziniu Yu, Shouwen Chen, Zhenyu Zhang, Lifang Ruan, Suxia Guo, Jingyuan Dai, Lin Li, Ziduo Liu