Abstract: A set of primers and probes for simultaneous detection of Cymbidium mosaic virus (CymMV), Odontoglossum ringspot virus (ORSV), and Cymbidium ringspot virus (CymRSV) and a method for detecting CymMV, ORSV, and CymRSV, along with a method for their detection, are disclosed. The method involves designing multiplex real-time quantitative PCR detection primers and probes for CymMV, ORSV, and CymRSV and applying these primers and probes to the real-time quantitative PCR simultaneous detection of CymMV, ORSV, and CymRSV. It allows for faster detection of CymMV, ORSV, and CymRSV, taking only one-third of the time compared to uniplex real-time quantitative PCR technology, thereby reducing testing costs by approximately ? to ½ for each sample. The primers and probes are highly specific and sensitive, with a sensitivity as low as 1 to 10 copies. It provides an efficient and feasible detection method for early detection and prevention of CymMV, ORSV, and CymRSV.

Abstract: Disclosed are an S1AP signaling transmission method and apparatus. The method comprises: an intelligent mobile edge device receives an SCTP message carrying uplink S1AP signaling of a terminal sent by a Small Cell, determines an operator network corresponding to the terminal according to an S1AP ID carried in the uplink S1AP signaling, and sends the SCTP message carrying the uplink S1AP signaling to the He-GW of the operator network; the intelligent mobile edge device receives an SCTP message carrying downlink S1AP signaling of the terminal sent by the He-GW of the operator network, determines the Small Cell corresponding to the terminal according to an S1AP ID carried in the downlink S1AP signaling, and sends the SCTP message carrying the downlink S1AP signaling to the Small Cell. In this way, a Small Cell may access networks of multiple operators to provide network services for terminals of different operators.