Patents by Inventor Liguang Xu
Liguang Xu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240132517Abstract: A compound represented by formula (I), or a pharmaceutically acceptable salt, solvate, active metabolite, polymorph, isotope marker, isomer or prodrug thereof. Also provided are a pharmaceutical composition comprising the same, and the use of the compound and the pharmaceutical composition in the preparation of a medicament for treating tyrosine kinase-mediated diseases. The provided compound and the pharmaceutical composition thereof have significant tyrosine kinase inhibitory activity, can overcome tumor drug resistance, can break through the blood-brain barrier, and further have excellent pharmacokinetic properties and excellent oral bioavailability, and can be administered in a small dose, thereby reducing treatment cost for patients and possible side effects; therefore, the provided compound and the pharmaceutical composition thereof have great application potentials.Type: ApplicationFiled: February 9, 2022Publication date: April 25, 2024Applicant: GOHARMONY THERAPEUTICS (SHENZHEN) CO., LTD.Inventors: Liguang DAI, Wei HU, Changxin DONG, Yanqing YANG, Wei WU, Wenlian XU
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Publication number: 20230167196Abstract: The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.Type: ApplicationFiled: November 9, 2021Publication date: June 1, 2023Inventors: Chuanlai XU, Jingjing YAO, Hua KUANG, Liguang XU, Maozhong SUN, Xiaoling WU, Liqiang LIU, Wei MA, Jianping ZHU, Changlong HAO, Shanshan SONG, Yongming HU, Aihong WU, Lingling GUO, Xinxin XU
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HYBRIDOMA CELL LINE OF SECRETING CYPROHEPTADINE MONOCLONAL ANTIBODIES AND PREPARATION METHOD THEREOF
Publication number: 20230133974Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.Type: ApplicationFiled: December 27, 2022Publication date: May 4, 2023Inventors: Chuanlai XU, Hua KUANG, Wei JIANG, Liguang XU, Wei MA, Liqiang LIU, Xiaoling WU, Shanshan SONG, Yongming HU -
Hybridoma cell line that secrets cyproheptadine monoclonal antibodies and preparation method thereof
Patent number: 11603414Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.Type: GrantFiled: December 17, 2018Date of Patent: March 14, 2023Assignee: JIANGNAN UNIVERSITYInventors: Chuanlai Xu, Hua Kuang, Wei Jiang, Liguang Xu, Wei Ma, Liqiang Liu, Xiaoling Wu, Shanshan Song, Yongming Hu -
Publication number: 20220010029Abstract: A hybridoma cell strain secreting anti-dinitolmide monoclonal antibodies deposited at China General Microbiological Culture Collection Center (CGMCC) at No. 1 West Beichen Road, Chaoyang District, Beijing 100101, China on Nov. 28, 2019, with deposit number of CGMCC No. 19165. It is classified as a monoclonal cell strain. The monoclonal antibody secreted by the hybridoma cell strain DAS3H10 has a good affinity and high sensitivity to dinitolmide. Because of IC50 to dinitolmide up to 9.01 ng/mL, the monoclonal antibody could be used to prepare dinitolmide immunoassay kit and colloidal gold test strip and provides a powerful detection method and means for the detection of dinitolmide in animal-derived foods.Type: ApplicationFiled: July 6, 2021Publication date: January 13, 2022Inventors: Chuanlai XU, JIE LIU, HUA KUANG, Liguang XU, Maozhong SUN, Liqiang LIU, Xiaoling WU, Changlong HAO, Shanshan SONG, Yongming HU, Qiankun ZHENG
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Patent number: 10882896Abstract: A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 ?g/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.Type: GrantFiled: July 21, 2020Date of Patent: January 5, 2021Assignee: JIANGNAN UNIVERSITYInventors: Hua Kuang, Chuanlai Xu, Aihong Wu, Liguang Xu, Wei Ma, Liqiang Liu, Xiaoling Wu, Shanshan Song, Yongming Hu
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Publication number: 20200347119Abstract: A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 ?g/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.Type: ApplicationFiled: July 21, 2020Publication date: November 5, 2020Inventors: Hua KUANG, Chuanlai XU, Aihong WU, Liguang XU, Wei MA, Liqiang LIU, Xiaoling WU, Shanshan SONG, Yongming HU
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Patent number: 10808041Abstract: The present disclosure discloses a lincosamides universal monoclonal antibody hybridoma cell strain and application thereof, and belongs to the technical field of food safety immunological detection. According to the present disclosure, a clindamycin chlorine-substituted derivative is used as a hapten, the hapten is coupled with bovine serum albumin (BSA) by an activated ester method to obtain an immunizing antigen, and after being uniformly mixed with a Freund's adjuvant, the immunizing antigen is subcutaneously injected to immunize BALB/c mice; clindamycin is coupled with ovalbumin (OVA) by a carbonyl diimidazole (CDI) method to be used as a coating antigen used for detecting mouse serums and a cell supernatant. The spleen cells of the immunized mice are fused with mouse myeloma cells by a PEG method, and screened by indirect ELISA and indirect competitive ELISA and subcloned three times to obtain a population-selective hybridoma cell strain.Type: GrantFiled: November 28, 2019Date of Patent: October 20, 2020Assignee: Jiangnan UniversityInventors: Hua Kuang, Lingling Guo, Chuanlai Xu, Liguang Xu, Wei Ma, Liqiang Liu, Shanshan Song, Xiaoling Wu
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Patent number: 10759845Abstract: A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody with CGMCC No. 18525 is prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 ?g/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.Type: GrantFiled: December 17, 2018Date of Patent: September 1, 2020Inventors: Hua Kuang, Chuanlai Xu, Aihong Wu, Liguang Xu, Wei Ma, Liqiang Liu, Xiaoling Wu, Shanshan Song, Yongming Hu
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Hybridoma cell line of secreting clarithromycin monoclonal antibodies and preparation method thereof
Patent number: 10696748Abstract: A hybridoma cell line of secreting clarithromycin monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14696 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with clarithromycin complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three subclones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to clarithromycin (value of IC50 is 0.3 ng/ml), being suitable for detection of clarithromycin in food.Type: GrantFiled: April 25, 2019Date of Patent: June 30, 2020Assignee: JIANGNAN UNIVERSITYInventors: Chuanlai Xu, Hua Kuang, Lu Zeng, Liguang Xu, Liqiang Liu, Shanshan Song, Xiaoling Wu, Yongming Hu -
Publication number: 20200095337Abstract: The present disclosure discloses a lincosamides universal monoclonal antibody hybridoma cell strain and application thereof, and belongs to the technical field of food safety immunological detection. According to the present disclosure, a clindamycin chlorine-substituted derivative is used as a hapten, the hapten is coupled with bovine serum albumin (BSA) by an activated ester method to obtain an immunizing antigen, and after being uniformly mixed with a Freund's adjuvant, the immunizing antigen is subcutaneously injected to immunize BALB/c mice; clindamycin is coupled with ovalbumin (OVA) by a carbonyl diimidazole (CDI) method to be used as a coating antigen used for detecting mouse serums and a cell supernatant. The spleen cells of the immunized mice are fused with mouse myeloma cells by a PEG method, and screened by indirect ELISA and indirect competitive ELISA and subcloned three times to obtain a population-selective hybridoma cell strain.Type: ApplicationFiled: November 28, 2019Publication date: March 26, 2020Inventors: Hua KUANG, Lingling GUO, Chuanlai XU, Liguang XU, Wei MA, Liqiang LIU, Shanshan SONG, Xiaoling WU
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Patent number: 10562979Abstract: A hybridoma cell line of secreting meloxicam monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14700 belongs to the field of food safety immunological detection. The. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with meroxicam complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to meloxicam (value of IC50 is 0.1 ng/ml), being suitable for detection of meroxicam in food.Type: GrantFiled: December 17, 2018Date of Patent: February 18, 2020Assignee: JIANGNAN UNIVERSITYInventors: Hua Kuang, Chuanlai Xu, Lu Lin, Liguang Xu, Wei Ma, Liqiang Liu, Xiaoling Wu, Shanshan Song, Yongming Hu
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Publication number: 20200048329Abstract: A hybridoma cell strain secreting a nifursolol residue marker monoclonal antibody with CGMCC No. 14698 is prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursolol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 ?g/L) to the nifursolol residue marker and can be used for residue detection of the nifursolol residual marker in food.Type: ApplicationFiled: December 17, 2018Publication date: February 13, 2020Inventors: Hua KUANG, Chuanlai XU, Aihong WU, Liguang XU, Wei MA, Liqiang LIU, Xiaoling WU, Shanshan SONG, Yongming HU
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Publication number: 20200040106Abstract: A hybridoma cell line of secreting meloxicam monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14700 belongs to the field of food safety immunological detection. The. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with meroxicam complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to meloxicam (value of IC50 is 0.1 ng/ml), being suitable for detection of meroxicam in food.Type: ApplicationFiled: December 17, 2018Publication date: February 6, 2020Inventors: Hua KUANG, Chuanlai XU, Lu LIN, Liguang XU, Wei MA, Liqiang LIU, Xiaoling WU, Shanshan SONG, Yongming HU
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Hybridoma cell line of secreting clarithromycin monoclonal antibodies and preparation method thereof
Publication number: 20190284304Abstract: A hybridoma cell line of secreting clarithromycin monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14696 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with clarithromycin complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three subclones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to clarithromycin (value of IC50 is 0.3 ng/ml), being suitable for detection of clarithromycin in food.Type: ApplicationFiled: April 25, 2019Publication date: September 19, 2019Inventors: Chuanlai XU, Hua KUANG, Lu ZENG, Liguang XU, Liqiang LIU, Shanshan SONG, Xiaoling WU, Yongming HU -
Hybridoma cell line of secreting cyproheptadine monoclonal antibodies and preparation method thereof
Publication number: 20190284303Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.Type: ApplicationFiled: December 17, 2018Publication date: September 19, 2019Inventors: Chuanlai XU, Hua KUANG, Wei JIANG, Liguang XU, Wei MA, Liqiang LIU, Xiaoling WU, Shanshan SONG, Yongming HU -
Hybridoma cell line of secreting clarithromycin monoclonal antibodies and preparation method thereof
Patent number: 10323100Abstract: A hybridoma cell line of secreting clarithromycin monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14696 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with clarithromycin complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three subclones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to clarithromycin (value of IC50 is 0.3 ng/ml), being suitable for detection of clarithromycin in food.Type: GrantFiled: December 17, 2018Date of Patent: June 18, 2019Assignee: JIANGNAN UNIVERSITYInventors: Chuanlai Xu, Hua Kuang, Lu Zeng, Liguang Xu, Liqiang Liu, Shanshan Song, Xiaoling Wu, Yongming Hu -
Patent number: 8822184Abstract: Multiple properties of plasmonic assemblies are determined by their geometrical organization. This patent focuses on the formation of Janus structure of the asymmetric assembly structure of the gold nanorods and gold nanoparticles. Chiral structure of gold nanorods and gold nanoparticles can be obtained through the characterization of optical spectra of the Janus structure. And it opens the door for the explanation of the mechanism of the chirality, plays a strong guiding role in the negative refractive material above and has good application prospects.Type: GrantFiled: May 2, 2012Date of Patent: September 2, 2014Inventors: Libing Wang, Chuanlai Xu, Liguang Xu, Xiaoling Wu
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Publication number: 20130071882Abstract: Multiple properties of plasmonic assemblies are determined by their geometrical organization. This patent focuses on the formation of Janus structure of the asymmetric assembly structure of the gold nanorods and gold nanoparticles. Chiral structure of gold nanorods and gold nanoparticles can be obtained through the characterization of optical spectra of the Janus structure. And it opens the door for the explanation of the mechanism of the chirality, plays a strong guiding role in the negative refractive material above and has good application prospects.Type: ApplicationFiled: May 2, 2012Publication date: March 21, 2013Inventors: Libing WANG, Chuanlai Xu, Liguang Xu, Xiaoling Wu