Abstract: A tissue repair device and a method for using the same are provided. The tissue repair device includes a body portion and at least one wire. The body portion includes an inner layer and an outer layer. The inner layer is close to a tissue, wherein the inner layer includes a hydrophilic structure, and the outer layer includes a hydrophobic structure. The wire is connected to the body portion to fix the body portion to the tissue.

Abstract: A cell purification module, configured to purify multiple cells from a fluid sample is provided. The cell purification module includes a hollow column, multiple hollow fiber membranes, at least one first magnetic component, a fluid sample inlet end, and a fluid sample outlet end. The hollow column has a first opening, a second opening, and an accommodating space connecting the first opening and the second opening. The hollow fiber membranes are disposed in the accommodating space and each hollow fiber membrane has multiple pores. The first magnetic component is disposed at a periphery of the hollow column. The fluid sample inlet end and the fluid sample outlet end are respectively disposed at two ends of the hollow column. The hollow fiber membranes extend in an axial direction of the hollow column, and are arranged in a radial direction of the hollow column. A cell purification system is also provided.

Abstract: A tissue scaffold for use in a tendon and/or ligament is provided, which includes a weave formed by interlacing warp yarns and weft yarns, wherein the warp yarns include a plurality of fibers with an alternative shaped cross section structure, and the weave includes: a main body area with a bioactive component formed on the fiber surface, and a fixed area comprises the weft yarn having a bioceramic material. The tissue scaffold prepared in the present disclosure has the characteristics of stimulating the growth of tissues and inducing tissue repair, effectively improving the ability of tissue regeneration and bone healing, and is beneficial to the reconstruction of the tendon and/or ligament.

Abstract: A tissue repair device and a method for using the same are provided. The tissue repair device includes a body portion and at least one wire. The body portion includes an inner layer and an outer layer. The inner layer is close to a tissue, wherein the inner layer includes a hydrophilic structure, and the outer layer includes a hydrophobic structure. The wire is connected to the body portion to fix the body portion to the tissue.

Abstract: The disclosure provides a use of hydrogel composition for alleviating degenerative joint and tendon tear. The hydrogel composition includes 100 parts by weight of therapeutic agent and 120-380 parts by weight of biodegradable copolymer, wherein the therapeutic agent comprises platelet-rich plasma (PRP), doxorubicin, transforming growth factor, bovine serum albumin, or a combination thereof. The biodegradable copolymer has a structure of Formula (I) or Formula (II): wherein A is a hydrophilic polyethylene glycol polymer; B is a hydrophobic polyester polymer; BOX is a bifunctional group monomer of 2, 2?-bis(2-oxazoline) used for coupling the blocks A-B or B-A-B; and n is 0 or an integer greater than 0.

Abstract: The disclosure provides a powder form and a hydrogel composition for alleviating degenerative joint and tendon tear. The powder form consists essentially of a 120-380 parts by weight biodegradable copolymer; 15-75 parts by weight urea; and 100 parts by weight platelet-rich plasma; wherein the biodegradable copolymer has a structure of formula (I) or formula (II): wherein A comprises a hydrophilic polyethylene glycol polymer; B comprises a hydrophobic polyester polymer; BOX is bifunctional group monomer of 2, 2?-bis(2-oxazoline) used for coupling the blocks AB or BAB; and n is an integer greater than or equal to 0.

Abstract: A method for preparing a hepatocyte includes the following steps: providing a liver progenitor cell, proliferating the liver progenitor cell in a medium supplemented with nicotinamide, insulin-transferrin-selenium (ITS) and EGF, and inducing the liver progenitor cell into the hepatocyte having a hepatic cord-like structure morphology. In one embodiment, the method further includes providing a pluripotent stem cell and differentiating the pluripotent stem cell into the liver progenitor cell.

Abstract: Cultured hepatocytes with hepatic-cord structure and the applications were disclosed. Also the disclosure performed the method for obtaining the cultured hepatocytes with hepatic-cord structure from pluripotent stem cells and progenitor cells.

Abstract: A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set.

Abstract: A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set.

Abstract: A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set.

Abstract: A cell colonies dissecting and transplanting apparatus comprises: a base, a platform, a feeding mechanism having at least three axis feeding means, a movable holder, a cutting means, a transplanting means, and a CCD image capturing means. The platform having the CCD image capturing means therebottom is disposed onto the base, and on the platform is defined with the holder for receiving a plurality of loading trays, so as to the original cell colonies and a plurality of media boards of subculture can be placed on the loading trays of holder, the holder can displace from a first axis feeding means and a second axis feeding means of feeding mechanism to moveably align with a first axis and a second axis.

Abstract: Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ray radiation or by mitomycin C treatment. The cultured human embryonic stem cells remain substantially undifferentiated and maintain their pluripotency to differentiate into three germ layer cells.

Abstract: A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set.

Abstract: An aspiration device for cells or tissues loaded on a container includes a tubular body, a scraper disposed on the tubular body. The scraper scrapes cells or tissues from the container, and the scraped cells or tissues are suctioned into the tubular body. The scraper includes a connecting portion connected to the tubular body, an elastic portion connected to the connecting portion, and a scraping end connected to the elastic portion. When the scraper scrapes the cells or the tissues, the elastic portion deforms elastically to enable the scraping end to abut the surface of the container, thus, the cells or tissues are scraped.

Abstract: A dissection device for organisms includes a table bearing an organism and at least one blade lifted or lowered to approach the table. The blade includes a connecting portion, a cutting portion having a curved edge, and a neck portion connecting the connecting portion and the cutting portion. When the blade is lowered to contact the table, the cutting portion is rotated with respect to the neck portion to have rolling contact with the table from a first point to a second point of the edge.

Abstract: A device for dissecting an object, such as a cell colony includes a handle and an incision knife module that can dissociate the colony into several colony pieces. In accordance with features of the invention, users can use this dissecting device to cut an object into several pieces quickly and smoothly at a time.

Abstract: A cell colonies dissecting and transplanting apparatus comprises: a base, a platform, a feeding mechanism having at least three axis feeding means, a movable holder, a cutting means, a transplanting means, and a CCD image capturing means. The platform having the CCD image capturing means therebottom is disposed onto the base, and on the platform is defined with the holder for receiving a plurality of loading trays, so as to the original cell colonies and a plurality of media boards of subculture can be placed on the loading trays of holder, the holder can displace from a first axis feeding means and a second axis feeding means of feeding mechanism to moveably align with a first axis and a second axis.

Abstract: Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ray radiation or by mitomycin C treatment. The cultured human embryonic stem cells remain substantially undifferentiated and maintain their pluripotency to differentiate into three germ layer cells.

Abstract: The present invention discloses an improved culture system for proliferative and undifferentiated growth of human embryonic stem cells, comprising an extracellular matrix prepared from feeder cells and a conditioned medium preconditioned by feeder cells. This invention also relates to a culture method utilizing the foregoing culture system.