Patents by Inventor Lihua Jian

Lihua Jian has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of screening drugs that promote LOXL1 expression
    Patent number: 9481915
    Abstract: The present invention discloses a drug screening method, drugs promoting extracellular matrix protein crosslinking and their applications, the said drug screening method, is used to screen out materials promoting the expression of LOXL1 gene, wherein, the said drug screening method contains the following steps: A, Construct the 2nd generation lentiviral vector used to control the ZsGreen expression by human LOXL 1 gene promoter. B, Infect human fibroblasts with the 2nd generation lentiviral vector, and construct the new human fibroblasts which integrate PLOXL1-ZsGreen components. C, Drug screening: Inoculate the said human fibroblasts integrating PLOXL1-ZsGreen components into culture medium. Add the analyte into the cell culture medium containing human fibroblast cells. After culturing, detect the green fluorescence intensity of these fibroblast cells, then decide if the analyte promotes LOXL1 gene expression by checking if the green fluorescence intensity is increased.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: November 1, 2016
    Assignee: SUZHOU BIOWISETECH CO., LTD.
    Inventors: Xiaoqing Liu, Ming Ying, Lihua Jian
  • DRUG SCREENING METHOD, DRUGS PROMOTING EXTRACELLULAR MATRIX CROSSLINKING AND THE APPLICATIONS THEREOF
    Publication number: 20140302181
    Abstract: The present invention discloses a drug screening method, drugs promoting extracellular matrix protein crosslinking and their applications, the said drug screening method, is used to screen out materials promoting the expression of LOXL1 gene, wherein, the said drug screening method contains the following steps: A, Construct the 2nd generation lentiviral vector used to control the ZsGreen expression by human LOXL 1 gene promoter. B, Infect human fibroblasts with the 2nd generation lentiviral vector, and construct the new human fibroblasts which integrate PLOXL1-ZsGreen components. C, Drug screening: Inoculate the said human fibroblasts integrating PLOXL1-ZsGreen components into culture medium. Add the analyte into the cell culture medium containing human fibroblast cells. After culturing, detect the green fluorescence intensity of these fibroblast cells, then decide if the analyte promotes LOXL1 gene expression by checking if the green fluorescence intensity is increased.
    Type: Application
    Filed: December 28, 2012
    Publication date: October 9, 2014
    Inventors: Xiaoqing Liu, Ming Ying, Lihua Jian