Abstract: A growth-promoting bacterial agent capable of improving content of soybean oil, preparation method, and use thereof are provided. The growth-promoting bacterial agent is fermented by a DW1 strain, the classification name of the DW1 strain is Bacillus sp., the DW1 strain is deposited in the China Center for Type Culture Collection on Jul. 16, 2021, and the deposit number is CCTCC NO: M 2021889. The growth-promoting bacterial agent of the present disclosure has the ability to dissolve insoluble phosphorus and insoluble potassium and can increase content of readily available phosphorus and readily available potassium in the soil, which has an obvious promoting effect on the growth of soybean and improves soybean yield. The growth-promoting bacterial agent of the present disclosure promotes bacteria and can significantly improve the content of soybean oil. At the same time, it has an affinity of soybean agglutinin and can agglutinate with the soybean agglutinin.

Abstract: The present invention provides a soybean anti-pod-shattering major QTLqPD05, and a mapping method and application thereof, and belongs to the field of QTL mapping. The soybean anti-pod-shattering major QTL is mapped on the chromosome 5 of soybean at a physical position between 40448596-40703417. For the method for mapping the soybean anti-pod-shattering major QTL, a SLAF marker is screened at the whole genome level of the soybean by utilizing a SLAF-seq sequencing technology, so as to explore the QTLs related to pod shattering from this population. By using a material of a RIL7 population which has pod-shattering soybean and anti-pod-shattering soybean as the parents, a high-density genetic linkage map covering the whole genome of soybean is constructed, and QTL mapping of the anti-pod-shattering trait is carried out on this population to obtain QTLs related to anti-pod-shattering.

Abstract: The present invention provides a soybean genetic transformation method using PMI as selectable gene, and relates to the technical field of genetic engineering. In the soybean genetic transformation method of the present invention, using the PMI gene as a selectable marker, soybean explants are infected by recombinant Agrobacterium with the PMI gene and a target gene, followed by co-culture; without recovery culture, the co-cultured explants are directly selected by a selective medium supplemented with mannose, where transformed explants with the PMI gene grow normally under selection pressure of mannose, while non-transformed explant growth is inhibited, thereby selecting successfully transformed positive plants; after shoot elongation and transplantation of the positive plants obtained, genetically transformed soybean plants are obtained successfully.

Abstract: A growth-promoting bacterial agent capable of improving content of soybean oil, preparation method, and use thereof are provided. The growth-promoting bacterial agent is fermented by a DW1 strain, the classification name of the DW1 strain is Bacillus sp., the DW1 strain is deposited in the China Center for Type Culture Collection on Jul. 16, 2021, and the deposit number is CCTCC NO: M 2021889. The growth-promoting bacterial agent of the present disclosure has the ability to dissolve insoluble phosphorus and insoluble potassium and can increase content of readily available phosphorus and readily available potassium in the soil, which has an obvious promoting effect on the growth of soybean and improves soybean yield. The growth-promoting bacterial agent of the present disclosure promotes bacteria and can significantly improve the content of soybean oil. At the same time, it has an affinity of soybean agglutinin and can agglutinate with the soybean agglutinin.

Abstract: The present invention provides a soybean genetic transformation method using PMI as selectable gene, and relates to the technical field of genetic engineering. In the soybean genetic transformation method of the present invention, using the PMI gene as a selectable marker, soybean explants are infected by recombinant Agrobacterium with the PMI gene and a target gene, followed by co-culture; without recovery culture, the co-cultured explants are directly selected by a selective medium supplemented with mannose, where transformed explants with the PMI gene grow normally under selection pressure of mannose, while non-transformed explant growth is inhibited, thereby selecting successfully transformed positive plants; after shoot elongation and transplantation of the positive plants obtained, genetically transformed soybean plants are obtained successfully.

Abstract: The present invention provides a soybean anti-pod-shattering major QTLqPD05, and a mapping method and application thereof, and belongs to the field of QTL mapping. The soybean anti-pod-shattering major QTL is mapped on the chromosome 5 of soybean at a physical position between 40448596-40703417. For the method for mapping the soybean anti-pod-shattering major QTL, a SLAF marker is screened at the whole genome level of the soybean by utilizing a SLAF-seq sequencing technology, so as to explore the QTLs related to pod shattering from this population. By using a material of a RIL7 population which has pod-shattering soybean and anti-pod-shattering soybean as the parents, a high-density genetic linkage map covering the whole genome of soybean is constructed, and QTL mapping of the anti-pod-shattering trait is carried out on this population to obtain QTLs related to anti-pod-shattering.