Abstract: An umbilical cord artery perivascular stem cell injection solution for treating ischemic diseases including suspended umbilical cord artery perivascular stem cells and a solvent and a preparation method thereof are provided. The solvent may be umbilical cord blood-derived platelet-rich plasma or injectable PBS containing human serum albumin. The preparation method includes: cultivating an umbilical cord artery tissue to derive perivascular stem cells, performing an enzymatic digestion to obtain individual umbilical cord artery perivascular stem cells, followed by washing twice with PBS, and gently mixing evenly with the solvent to obtain the umbilical cord artery perivascular stem cell injection solution. The cells are taken from the umbilical cord of medical waste and have the characteristics of easy separation, fast proliferation and low immunogenicity.