Abstract: Provided herein are new and useful placental derived natural killer (NK) Cell or population thereof, or one or more cells within the population that express a cleavage resistant CD 16 polypeptide and a secreted IL-15 polypeptide, as well as uses thereof.

Abstract: Provided herein is a method of ameliorating muscle loss in a subject induced from the treatment of the subject with a GLP-1 agonist with the administration to the subject of a therapeutically effective amount of a placenta derived biological material having a continuous lipid bilayer membrane

Abstract: Provided herein are cells or populations of cells comprising a polynucleotide encoding a cleavage resistant CD16 polypeptide. Also provided herein are methods of suppressing the proliferation of tumor cells such as HER2+ and methods of treating cancers, such as HER2+ in a subject with populations of placental-derived natural killer cells or placental-derived T cells comprising a cleavage resistant CD16. The natural killer cells, such as CYNK cells, can be placental CD34+ cell-derived natural killer (NK) cells. The placental-derived T cells can be isolated from cord blood or from placental perfusate.

Abstract: A rapid detection method for abrin toxin is provided. The rapid detection method for abrin toxin includes the following steps: reacting an oligonucleotide chain substrate labeled with a fluorescent group and a quenching group, a buffer solution, a BSA solution, a to-be-detected sample and sterile water, incubating at a constant temperature, detecting a fluorescence signal value, and determining whether the abrin toxin exists based on a difference between a change in final fluorescence signal value and a change in final fluorescence signal value of a negative control; where the sample is judged as a positive sample when an average value of the detected final fluorescence signal values is greater than an average value of the final fluorescence signal values of the negative control by +3 times standard deviation. According to the detection method, the direct addition of the sample into the reaction system can reach the sensitivity of 30 ng/mL.

Abstract: A rapid detection method for abrin toxin is provided. The rapid detection method for abrin toxin includes the following steps: reacting an oligonucleotide chain substrate labeled with a fluorescent group and a quenching group, a buffer solution, a BSA solution, a to-be-detected sample and sterile water, incubating at a constant temperature, detecting a fluorescence signal value, and determining whether the abrin toxin exists based on a difference between a change in final fluorescence signal value and a change in final fluorescence signal value of a negative control; where the sample is judged as a positive sample when an average value of the detected final fluorescence signal values is greater than an average value of the final fluorescence signal values of the negative control by +3 times standard deviation. According to the detection method, the direct addition of the sample into the reaction system can reach the sensitivity of 30 ng/mL.

Abstract: A rapid detection method for ricin toxin is provided. The rapid detection method for ricin toxin comprises reacting an adenine-containing oligonucleotide chain substrate labeled with a fluorescent group and a quenching group, a buffer solution, a BSA solution, a to-be-detected sample and sterile water, incubating at a constant temperature, detecting a fluorescence signal value, and determining whether the ricin toxin exists based on a difference between an average value of final fluorescence signal values and an average value of final fluorescence signal values of a negative control; wherein the sample is judged as a positive sample when the average value of the detected final fluorescence signal values is greater than the average value of the final fluorescence signal values of the negative control by +3 times standard deviation.

Abstract: A superconducting nanowire photon detection array adjusts a number of the array elements, a lens array that 1) splits transmitted lights into multiple beams that equals the number of the array elements, and are converged in to a superconducting nanowire detection area; 2) a pulsed laser detects a surface of an object, transmits reflected different light pulses by the surface of the object through the lens array, and records a round-trip time of each photon; 3) collects the photons detected by each array element, takes the array elements as pixels and calculates a gray value of the pixels; and 4) plots a gray-scale image by taking the pixels as pixel points, calculates a distance between the object and the pixel points, and reconstructs a three-dimensional image of the object.

Abstract: The present invention discloses a design for reducing a dark count rate of a superconducting nanowire single photon detector (SNSPD) based on a two-wire structure, which includes: intertwining two niobium nitride nanowires that are not crossed to form an SNSPD of a two-wire structure; regulating and controlling behaviors of one nanowire by adopting the other nanowire, and regulating bias current to be close to superconducting critical current; introducing an optical signal into a photosensitive area of the detector by adopting an optical fiber; outputting two channels of signals respectively through the two nanowires to make the dark count rates of the two nanowires mutually excited; and through a voltage comparator and an exclusive-OR gate, reducing a dark count rate signal, and retaining a photon response signal.

Abstract: Provided herein are methods of producing natural killer (NK) cells and/or ILC3 cells using a three-stage expansion and differentiation method with media comprising stem cell mobilizing factors. Also provided herein are methods of suppressing tumor cell proliferation using the NK cells and/or ILC3 cells and the NK cell and/or ILC3 cell populations produced by the three-stage methods described herein, as well as methods of treating individuals having cancer or a viral infection, comprising administering the NK cells and/or ILC3 cells and the NK cell and/or ILC3 cell populations produced by the three-stage methods described herein to an individual having the cancer or viral infection.

Abstract: A superconducting nanowire photon detection array adjusts a number of the array elements, a lens array that 1) splits transmitted lights into multiple beams that equals the number of the array elements, and are converged in to a superconducting nanowire detection area; 2) a pulsed laser detects a surface of an object, transmits reflected different light pulses by the surface of the object through the lens array, and records a round-trip time of each photon; 3) collects the photons detected by each array element, takes the array elements as pixels and calculates a gray value of the pixels; and 4) plots a gray-scale image by taking the pixels as pixel points, calculates a distance between the object and the pixel points, and reconstructs a three-dimensional image of the object.

Abstract: Provided herein are methods of producing natural killer (NK) cells and/or ILC3 cells using a three-stage expansion and differentiation method with media comprising stem cell mobilizing factors. Also provided herein are methods of suppressing tumor cell proliferation using the NK cells and/or ILC3 cells and the NK cell and/or ILC3 cell populations produced by the three-stage methods described herein, as well as methods of treating individuals having cancer or a viral infection, comprising administering the NK cells and/or ILC3 cells and the NK cell and/or ILC3 cell populations produced by the three-stage methods described herein to an individual having the cancer or viral infection.

Abstract: A fabrication method of a silicon nanoneedle array with ultra-high aspect ratio includes the following steps: spin-coating two photoresist layers of methyl methacrylate (MMA) and polymethyl methacrylate (PMMA) A2 on a silicon substrate; subjecting the silicon substrate coated with the two photoresist layers of MMA and PMMA A2 to electron beam lithography to form a photoresist pattern on the silicon substrate; subjecting the silicon substrate on which the photoresist pattern is formed to electron beam evaporation (EBE) to deposit an Al film layer on the silicon substrate; subjecting the silicon substrate on which the Al film layer is deposited to stripping to obtain an Al film array deposited on the silicon substrate, which provides a mask for the subsequent inductively coupled plasma (ICP) etching process; and subjecting the silicon substrate covered with the Al mask to ICP silicon etching to obtain a silicon nanoneedle array structure.

Abstract: Provided herein are methods of producing natural killer cells using a two-step expansion and differentiation method. Also provided herein are methods of suppressing tumor cell proliferation, of treating individuals having cancer or a viral infection, comprising administering the NK cells produced by the method to an individual having the cancer or viral infection.

Abstract: The present invention provides an isolated non-human placenta derived stem cell, wherein the stem cell expresses CD90 or wherein the stem cell expresses CD29. The present invention also provides a composition comprising an isolated non-human placenta derived stem cell of the invention or a population of isolated non-human placenta derived stem cells of the invention, and a carrier. The present invention also provides a composition of the invention for use in the manufacture of a medicament to reduce the incidence of rejection in a patient receiving a xenotransplant form a non-human donor. The present invention provides a method of treating a subject receiving a xenotransplant or xenotransfusion comprising the step of administering to the patient an effective amount of a non-human placenta derived stem cells.

Abstract: Provided herein are methods of using populations of natural killer (NK) cells and/or ILC3 cells derived from a population of hematopoietic stem or progenitor cells in methods for treating a viral infection, e.g., a coronavirus infection.

Abstract: A superconducting nanowire photon detection array adjusts a number of the array elements, a lens array as an photon alignment system, splits transmitted lights into a plurality of beams, and converges the plurality of beams to a superconducting nanowire detection area; detects a surface of an object by adopting a pulsed laser, transmits different light pulses reflected by the surface of the object through the lens array, and records a round-trip time of each photon; collects the photons detected by each array element, takes the array elements as picture elements, and calculates a gray value of the picture element; and plots a gray-scale image by taking the picture elements as pixel points, calculates a distance between the object and the pixel points, and reconstructs a three-dimensional image of the object and the distance between the object and the pixel points.

Abstract: Provided herein are methods of treating acute myeloid leukemia (AML) and multiple myeloma (MM) by administering an effective amount of a cell population comprising natural killer cells, wherein the cell population comprising natural killer cells is produced by a three-stage method comprising culturing a population of hematopoietic stem or progenitor cells in media comprising stem cell mobilizing factors, e.g., three-stage methods of producing NK cells in media comprising stem cell mobilizing factors starting with hematopoietic stem or progenitor cells from cells of the placenta, for example, from placental perfusate (e.g., human placental perfusate) or other tissues, for example, umbilical cord blood or peripheral blood. Further provided herein are methods of using the NK cells produced by the three-stage methods provided herein to suppress the proliferation of acute myeloid leukemia cells.

Abstract: Provided herein are methods of suppressing tumor cell proliferation, of treating individuals having cancer or a viral infection, comprising contacting the tumor cells, or administering to the individual, placental perfusate, placental perfusate cells, or natural killer cells, e.g., placenta-derived intermediate natural killer cells, with an immunomodulatory compound or thalidomide.

Abstract: Provided herein are methods of producing erythrocytes from hematopoietic cells, particularly hematopoietic cells from placental perfusate in combination with hematopoietic cells from umbilical cord blood, wherein the method results in accelerated expansion and differentiation of the hematopoietic cells to more efficiently produce administrable erythrocytes. Further provided herein is a bioreactor in which hematopoietic cell expansion and differentiation takes place.

Abstract: Provided herein are methods of treating a hematological disorder, a solid tumor, or an infectious disease in a subject in need thereof using natural killer cells in combination with a second agent, or using natural killer cells with genetic modifications for target specificity and/or homing specificity.