Abstract: A high-fidelity AsCpf1 mutant is obtained by performing a mutation on arginine at position 951 and/or 955 of a AsCpf1 protein amino acid sequence and replacing the same with an amino acid free of forming a hydrogen bond with DNA of a target site; and the amino acid sequence thereof is shown in SEQ ID NOS: 1-3. The encoding gene of the AsCpf1 mutant has a nucleotide sequence as shown in SEQ ID NO: 4 and can be used in the construction of a CRISPR/AsCpf1 gene editing system. A CRISPR/AsCpf1 gene editing system includes a gene encoding a AsCpf1 protein, and the AsCpf1 protein is the AsCpf1 mutant mentioned above. The CRISPR/AsCpf1 gene editing system can be used in lowering an off-target effect of gene editing. The novel AsCpf1 mutant not only retains the gene editing efficiency of wild-type AsCpf1, but also has a higher specificity than the wild-type AsCpf1.

Abstract: A high-fidelity AsCpf1 mutant is obtained by performing a mutation on arginine at position 951 and/or 955 of a AsCpf1 protein amino acid sequence and replacing the same with an amino acid free of forming a hydrogen bond with DNA of a target site; and the amino acid sequence thereof is shown in SEQ ID NOS: 1-3. The encoding gene of the AsCpf1 mutant has a nucleotide sequence as shown in SEQ ID NO: 4 and can be used in the construction of a CRISPR/AsCpf1 gene editing system. A CRISPR/AsCpf1 gene editing system includes a gene encoding a AsCpf1 protein, and the AsCpf1 protein is the AsCpf1 mutant mentioned above. The CRISPR/AsCpf1 gene editing system can be used in lowering an off-target effect of gene editing. The novel AsCpf1 mutant not only retains the gene editing efficiency of wild-type AsCpf1, but also has a higher specificity than the wild-type AsCpf1.

Abstract: An aerial imaging element in a teleprompter system, a teleprompter and a teleprompter device includes one or more optical elements that bend incident light of an image and form a real image in the air, and further includes one or more translucent reflectors. The aerial imaging element is convenient to manufacture and is low in cost; the aerial imaging element enables an image displayed therein to form a real image in the air, which can be seen only at a certain angle. The aerial imaging element is applied to the prompt of aspects, such as news broadcasting and lecture giving, which is convenient and does not influence viewing or video recording at the scene.

Abstract: An aerial imaging element in a teleprompter system, a teleprompter and a teleprompter device includes one or more optical elements that bend incident light of an image and form a real image in the air, and further includes one or more translucent reflectors. The aerial imaging element is convenient to manufacture and is low in cost; the aerial imaging element enables an image displayed therein to form a real image in the air, which can be seen only at a certain angle. The aerial imaging element is applied to the prompt of aspects, such as news broadcasting and lecture giving, which is convenient and does not influence viewing or video recording at the scene.