Patents by Inventor Linas Mazutis
Linas Mazutis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11958947Abstract: Provided herein are core-shell microcapsules useful for compartmentalizing biological molecules in solution. Also provided are processes for manufacturing core-shell microcapsules and methods for using core-shell microcapsules to compartmentalize and optionally process biological entities and molecules.Type: GrantFiled: January 25, 2023Date of Patent: April 16, 2024Assignee: DROPLET GENOMICS, UABInventors: Jonas Gasparavicius, Vaidotas Kiseliovas, Karolis Leonavicius, Linas Mazutis, Juozas Nainys, Andrius Sinkunas, Giedrius Zolubas, Rapolas Zilionis, Vaida Kurmauskaite, Greta Ruksnaityte, Ignas Ragaisis, Duygucan Gul
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Publication number: 20240085287Abstract: This invention relates to methods and systems for isolation of species in semi-permeable capsules and processing of encapsulated species through series of steps and/or reactions. To produce capsules, first aqueous two-phase system (ATPS) droplets are generated using microfluidics system. Then the hydrogel shell layer is hardened by inducing polymerization. As exemplified in this invention to achieve concentric ATPS droplet formation density-matched PEGDA and Dextran polymer solutions can be used. Once a capsule is formed, its composition can be changed by adding new reagents or replacing out old ones (e.g. by resuspending capsules in desired aqueous solution). The hydrogel shell of semi-permeable capsules can be dissolved at selected step during multi-step procedures to release the encapsulated species. This invention exemplifies isolation of individual cells within capsules and using the encapsulated cells for genotypic and phenotypic analysis.Type: ApplicationFiled: November 20, 2023Publication date: March 14, 2024Inventors: Linas Mazutis, Greta Stonyte, Karolis Leonavicius, Ausra Zelvyte
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Publication number: 20240026339Abstract: The present invention relates to amphiphiles and specifically the amphiphiles that can be used as cell lysis reagents. The invention reveals the amphiphiles as well as their combinations for efficiently lysing the mammalian cells under relatively mild conditions that are compatible with a reverse transcription and polymerase chain reaction. The invention reveals the use of mixture of amphiphiles to improve the cell lysis and obtain increased yields of copy DNA during the reverse transcription reaction. The invention also provides a method for increasing the diversity of gene and transcript capture during single-cell RNA-sequencing using droplet microfluidics.Type: ApplicationFiled: July 10, 2023Publication date: January 25, 2024Inventors: Linas Mazutis, Karolis Goda
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Patent number: 11860076Abstract: The present invention relates to methods and systems for isolation of species in semi-permeable capsules and processing of encapsulated species through series of steps and/or reactions. To produce capsules, first aqueous two-phase system (ATPS) droplets are generated using microfluidics system and then the hydrogel shell layer is hardened by inducing polymerization. As exemplified in this invention to achieve concentric ATPS droplet formation density-matched PEGDA and Dextran polymer solutions can be used. Once a capsule is formed, its composition can be changed by adding new reagents or replacing out old ones (e.g. by resuspending capsules in desired aqueous solution). The hydrogel shell of semi-permeable capsules can be dissolved at selected step during multi-step procedures in order to release the encapsulated species. The present invention exemplifies the isolation of individual cells within capsules and using the encapsulated cells for genotypic and phenotypic analysis.Type: GrantFiled: July 21, 2020Date of Patent: January 2, 2024Assignees: Vilnius University, Droplet GenomicsInventors: Linas Mazutis, Greta Stonyte, Karolis Leonavicius, Ausra Zelvyte
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Patent number: 11850589Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: GrantFiled: June 24, 2022Date of Patent: December 26, 2023Assignees: The Brigham and Women's Hospital, Inc., Boston, MA;, President and Fellows of Harvard College, Vilnius UniversityInventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz
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Publication number: 20230340210Abstract: Provided herein are core-shell microcapsules useful for compartmentalizing biological molecules in solution. Also provided are processes for manufacturing core-shell microcapsules and methods for using core-shell microcapsules to compartmentalize and optionally process biological entities and molecules.Type: ApplicationFiled: January 25, 2023Publication date: October 26, 2023Applicant: DROPLET GENOMICS, UABInventors: Jonas GASPARAVICIUS, Vaidotas KISELIOVAS, Karolis LEONAVICIUS, Linas MAZUTIS, Juozas NAINYS, Andrius SINKUNAS, Giedrius ZOLUBAS, Rapolas ZILIONIS, Vaida KURMAUSKAITE, Greta RUKSNAITYTE, Ignas RAGAISIS, Duygucan GUL
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Patent number: 11566214Abstract: Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels.Type: GrantFiled: February 17, 2020Date of Patent: January 31, 2023Assignees: BRIGHAM AND WOMEN'S HOSPITAL, INC., PRESIDENT AND FELLOWS OF HARVARD COLLEGE, VILNIUS UNIVERSITYInventors: Jonathan N. Thon, Joseph E. Italiano, Linas Mazutis, David A. Weitz
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Publication number: 20230009208Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: ApplicationFiled: June 24, 2022Publication date: January 12, 2023Inventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz
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Patent number: 11396016Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: GrantFiled: June 17, 2020Date of Patent: July 26, 2022Assignees: The Brigham and Women's Hospital, Inc., President and Fellows of Harvard College, Vilnius UniversityInventors: Joseph Italiano, Linas Mazutis, Jonathan Thon, David A. Weitz
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Patent number: 11326196Abstract: Disclosed is a system and method for production of DNA particles and use thereof. The DNA particles can be produced by amplification of nucleic acid molecule(s). Alternatively, DNA particles can be prepared by condensing multiple DNA molecules. The DNA condensation into a particle is mainly triggered by pyrophosphate and positively charged cations (e.g. magnesium). DNA particles can be applied for numerous biological applications but not limited to directed evolution, proteomics, drug delivery and imaging. DNA particles can be used to synthesize proteins using in vitro transcription/translation reaction.Type: GrantFiled: January 10, 2017Date of Patent: May 10, 2022Assignees: VILNIUS UNIVERSITY, ETH 7LIRICHInventors: Linas Mazutis, Greta Stonyte, Vaidotas Kiseliovas, Rapolas Zilionis, Arvydas Janulaitis, Robertas Galinis, Sabine Studer, Donald Hilvert
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Publication number: 20210379555Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.Type: ApplicationFiled: May 18, 2021Publication date: December 9, 2021Applicants: President and Fellows of Harvard College, Vilnius UniversityInventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
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Publication number: 20210340597Abstract: The present invention generally relates to droplet-based microfluidic devices, including systems, methods, and kits for amplifying or cloning within droplets. In some embodiments, the present invention is generally directed to systems, methods, or kits for amplifying a plurality of nucleic acids, e.g., without substantially selectively amplifying some nucleic acids over others. The nucleic acids may be contained within the droplets. In addition, in some embodiments, a plurality of microfluidic droplet containing a species of interest, such as a nucleic acid, may be mixed with microfluidic droplets free of the species, then pipetted or otherwise transferred such that, on average, a predetermined number of droplets containing species of interest is transferred.Type: ApplicationFiled: May 13, 2021Publication date: November 4, 2021Applicant: President and Fellows of Harvard CollegeInventors: David A. Weitz, John Heyman, Huidan Zhang, Linas Mazutis
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Patent number: 11052368Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.Type: GrantFiled: May 29, 2018Date of Patent: July 6, 2021Assignees: Vilnius University, President and Fellows of Harvard CollegeInventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
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Publication number: 20200400538Abstract: The present invention relates to methods and systems for isolation of species in semi-permeable capsules and processing of encapsulated species through series of steps and/or reactions. To produce capsules, first aqueous two-phase system (ATPS) droplets are generated using microfluidics system and then the hydrogel shell layer is hardened by inducing polymerization. As exemplified in this invention to achieve concentric ATPS droplet formation density-matched PEGDA and Dextran polymer solutions can be used. Once a capsule is formed, its composition can be changed by adding new reagents or replacing out old ones (e.g. by resuspending capsules in desired aqueous solution). The hydrogel shell of semi-permeable capsules can be dissolved at selected step during multi-step procedures in order to release the encapsulated species. The present invention exemplifies the isolation of individual cells within capsules and using the encapsulated cells for genotypic and phenotypic analysis.Type: ApplicationFiled: July 21, 2020Publication date: December 24, 2020Inventors: Linas Mazutis, Greta Stonyte, Karolis Leonavicius, Ausra Zelvyte
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Publication number: 20200316597Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: ApplicationFiled: June 17, 2020Publication date: October 8, 2020Inventors: Joseph Italiano, Linas Mazutis, Jonathan Thon, David A. Weitz
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Publication number: 20200255781Abstract: Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels.Type: ApplicationFiled: February 17, 2020Publication date: August 13, 2020Inventors: Jonathan N. Thon, Joseph E. Italiano, Linas Mazutis, David A. Weitz
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Patent number: 10710073Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: GrantFiled: May 24, 2019Date of Patent: July 14, 2020Assignees: Brigham and Women's Hospital, Inc., President and Fellows of Harvard College, Vilnius UniversityInventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz
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Patent number: 10596541Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.Type: GrantFiled: October 3, 2017Date of Patent: March 24, 2020Assignees: President and Fellows of Harvard College, Vilnius UniversityInventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
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Patent number: 10590373Abstract: Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels.Type: GrantFiled: March 30, 2015Date of Patent: March 17, 2020Assignees: BRIGHAM AND WOMEN'S HOSPITAL, INC., PRESIDENT AND FELLOWS OF HARVARD COLLEGE, VILNIUS UNIVERSITYInventors: Jonathan N. Thon, Joseph E. Italiano, Linas Mazutis, David A. Weitz
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Publication number: 20190283027Abstract: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.Type: ApplicationFiled: May 24, 2019Publication date: September 19, 2019Inventors: Joseph Italiano, Linas Mazutis, Jonathan N. Thon, David A. Weitz