Abstract: Cannabis plant material is extracted with a solvent matrix consisting of one or more organic solvents and optionally water. The cannabis plant material is agitated in the solvent matrix and then removed by filtration or centrifugation. Following extraction, the plant material is separated from the solvent matrix by centrifugation. The resulting extract is clarified by membrane filtration which also removes most of the water and water-soluble impurities. The resulting extract is dewaxed using membrane filtration and/or liquid-liquid extraction. Excess solvent and terpenoids are removed by means of molecular weight cutoff membrane filtration and the resulting cannabinoid solution is decarboxylated catalytically. Seed crystals are added to crystallize the cannabinoids.

Abstract: Cannabis plant material is extracted with a solvent matrix consisting of one or more organic solvents and optionally water. The cannabis plant material is agitated in the solvent matrix and then removed by filtration or centrifugation. Following extraction, the plant material is separated from the solvent matrix by centrifugation. The resulting extract is clarified by membrane filtration which also removes most of the water and water-soluble impurities. The resulting extract is dewaxed using membrane filtration and/or liquid-liquid extraction. Excess solvent and terpenoids are removed by means of molecular weight cutoff membrane filtration and the resulting cannabinoid solution is decarboxylated catalytically. Seed crystals are added to crystallize the cannabinoids.

Abstract: The production of high quality retinal pigmented epithelium (RPE) cells is necessary for research and potential therapeutic uses. Especially desirable are methods for the production of RPE cells using xeno-free culture conditions. Disclosed herein are novel methods for the production of RPE cells from pluripotent cells with high yields, including xeno-free production methods. Also provided are methods of efficiently isolating RPE cells from cultures containing heterogeneous cell types, allowing for substantially pure RPE cell cultures to be established. Additionally, novel methods for the cryopreservation of RPE cells are provided.

Abstract: The production of high quality retinal pigmented epithelium (RPE) cells is necessary for research and potential therapeutic uses. Especially desirable are methods for the production of RPE cells using xeno-free culture conditions. Disclosed herein are novel methods for the production of RPE cells from pluripotent cells with high yields, including xeno-free production methods. Also provided are methods of efficiently isolating RPE cells from cultures containing heterogeneous cell types, allowing for substantially pure RPE cell cultures to be established. Additionally, novel methods for the cryopreservation of RPE cells are provided.

Abstract: The present invention relates to a purified retinal pigmented epithelium derived neurotrophic factor composition and a method for purifying such a retinal pigmented epithelium neurotrophic factor. The present invention also relates to a recombinant DNA molecule comprising a gene encoding a retinal pigmented epithelium derived neurotrophic factor having the DNA sequence or the amino acid sequence in SEQ ID NO:1 and to an organism transformed with the recombinant DNA molecule. In addition, the present invention relates to a method of treating tumors, ocular diseases, nerve injuries, and conditions resulting from the activity of serine proteases, which comprises administering PEDF.

Abstract: Nucleic acids encoding the neurotrophic protein known as pigment epithelium-derived factor (PEDF), a truncated version of PEDF referred to as rPEDF, and equivalent proteins, vectors comprising such nucleic acids, host cells into which such vectors have been introduced, recombinant methods for producing PEDF, rPEDF, and equivalent proteins, the rPEDF protein and equivalent proteins of rPEDF and PEDF-BP, -BX and BA, and the PEDF protein produced by recombinant methods. Effects and uses of these variants on 1) neuronal differentiation (neurotrophic effect) 2) neuron survival (neuronotrophic effect) and 3) glial inhibition (gliastatic effect) are described.

Abstract: In an optical analysis system, six interference filters are mounted on a wheel on a cylindrical locus. The wheel rotates to move the filters in sequence through a beam of light and to vary the angle of incidence of the light to the filters as the filters move through the beam of light so that the wavelength passed by the filters is swept through a range of values. The intensity of light reflected from or transmitted through a test sample is detected to provide an indication of the reflective or transmissive optical density of the test sample at specific wavelengths. The angular position of each of the filters on the wheel is adjustable to make the range of light wavelengths transmitted by each filter adjustable.