Patents by Inventor Linda A. Rymarquis

Linda A. Rymarquis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11414670
    Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.
    Type: Grant
    Filed: April 13, 2020
    Date of Patent: August 16, 2022
    Assignee: Monsanto Technology LLC
    Inventors: Edwards M. Allen, Jayanand Boddu, Charles R. Dietrich, Alexander Goldshmidt, Miya Howell, Kevin R. Kosola, Anil Neelam, Thomas L. Slewinski, Tyamagondlu V. Venkatesh, Huai Wang, Sivalinganna Manjunath, Linda Rymarquis
  • Publication number: 20220195450
    Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through editing of a specific GA20 oxidase gene or locus to produce a genomic deletion or disruption that brings an antisense sequence of the GA20 oxidase gene under the control of a neighboring SAMT gene promoter. Modified plant cells and plants having a dominant allele reducing the expression or activity of one or more GA oxidase genes are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.
    Type: Application
    Filed: May 28, 2020
    Publication date: June 23, 2022
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Sivalinganna MANJUNATH, Linda A. RYMARQUIS, Thomas L. SLEWINSKI
  • Publication number: 20220195445
    Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through editing of specific subtypes of GA20 oxidase genes to introduce an antisense sequence or segment into the gene. Modified plant cells and plants having a dominant allele reducing the expression or activity of one or more GA oxidase genes are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.
    Type: Application
    Filed: May 28, 2020
    Publication date: June 23, 2022
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Sivalinganna MANJUNATH, Linda A. RYMARQUIS, Thomas L. SLEWINSKI
  • Publication number: 20220135991
    Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.
    Type: Application
    Filed: October 15, 2021
    Publication date: May 5, 2022
    Inventors: Brent Brower-Toland, Andrei Y. Kouranov, Rosemarie Kuehn, Richard J. Lawrence, Ervin D. Nagy, Linda Rymarquis, Veena Veena
  • Patent number: 11319550
    Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.
    Type: Grant
    Filed: April 13, 2020
    Date of Patent: May 3, 2022
    Assignee: Monsanto Technology LLC
    Inventors: Edwards M. Allen, Jayanand Boddu, Charles R. Dietrich, Alexander Goldshmidt, Miya Howell, Kevin R. Kosola, Anil Neelam, Thomas L. Slewinski, Tyamagondlu V. Venkatesh, Huai Wang, Sivalinganna Manjunath, Linda Rymarquis
  • Patent number: 11186843
    Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.
    Type: Grant
    Filed: February 27, 2015
    Date of Patent: November 30, 2021
    Assignee: Monsanto Technology LLC
    Inventors: Brent Brower-Toland, Andrei Y. Kouranov, Rosemarie Kuehn, Richard J. Lawrence, Ervin D. Nagy, Linda Rymarquis, Veena Veena
  • Publication number: 20210147862
    Abstract: This invention relates to recombinant nucleic constructs comprising a DNA binding domain, an endonuclease and a reverse transcriptase and methods of use thereof for modifying nucleic acids in plants.
    Type: Application
    Filed: October 23, 2020
    Publication date: May 20, 2021
    Inventors: Aaron Hummel, Joseph Matthew Watts, Shai Joshua Lawit, Nathaniel Graham, Larry Gilbertson, Ervin Nagy, Linda Rymarquis
  • Publication number: 20210040498
    Abstract: The present disclosure provides compositions and methods for the editing or mutating of specific subtypes of GA20 oxidase genes and specific zygosity combinations of those edits or mutations. Modified plants, and plant parts and cells thereof, having mutations reducing the expression or activity of GA20 oxidase genes are further provided with improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types. Methods are further provided for making modified plants, and plant parts and cells thereof, having one or more mutations in specific subtypes of GA20 oxidase genes.
    Type: Application
    Filed: February 15, 2019
    Publication date: February 11, 2021
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Sivalinganna MANJUNATH, Linda A. RYMARQUIS, Thomas L. SLEWINSKI
  • Publication number: 20210032649
    Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.
    Type: Application
    Filed: February 15, 2019
    Publication date: February 4, 2021
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Sivalinganna MANJUNATH, Linda A. RYMARQUIS, Thomas L. SLEWINSKI
  • Publication number: 20210032646
    Abstract: The present disclosure provides compositions and methods for the editing or mutating of specific subtypes of GA20 oxidase genes and specific zygosity combinations of those edits or mutations. Modified plants, and plant parts and cells thereof, having mutations reducing the expression or activity of GA20 oxidase genes are further provided with improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types. Methods are further provided for making modified plants, and plant parts and cells thereof, having one or more mutations in specific subtypes of GA20 oxidase genes.
    Type: Application
    Filed: February 15, 2019
    Publication date: February 4, 2021
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Sivalinganna MANJUNATH, Linda A. RYMARQUIS, Thomas L. SLEWINSKI
  • Publication number: 20200248200
    Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.
    Type: Application
    Filed: April 13, 2020
    Publication date: August 6, 2020
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Edwards M. ALLEN, Jayanand BODDU, Charles R. DIETRICH, Alexander GOLDSHMIDT, Miya HOWELL, Kevin R. KOSOLA, Anil NEELAM, Thomas L. SLEWINSKI, Tyamagondlu V. VENKATESH, Huai WANG, Sivalinganna MANJUNATH, Linda RYMARQUIS
  • Publication number: 20200248199
    Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.
    Type: Application
    Filed: April 13, 2020
    Publication date: August 6, 2020
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Edwards M. ALLEN, Jayanand BODDU, Charles R. DIETRICH, Alexander GOLDSHMIDT, Miya HOWELL, Kevin R. KOSOLA, Anil NEELAM, Thomas L. SLEWINSKI, Tyamagondlu V. VENKATESH, Huai WANG, Sivalinganna MANJUNATH, Linda RYMARQUIS
  • Patent number: 10724047
    Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.
    Type: Grant
    Filed: August 17, 2017
    Date of Patent: July 28, 2020
    Assignee: MONSANTO TECHNOLOGY LLC
    Inventors: Edwards M. Allen, Jayanand Boddu, Charles R. Dietrich, Alexander Goldshmidt, Miya Howell, Kevin R. Kosola, Sivalinganna Manjunath, Anil Neelam, Linda Rymarquis, Thomas L. Slewinski, Tyamagondlu V. Venkatesh, Huai Wang
  • Publication number: 20200140874
    Abstract: Disclosed herein are plants exhibiting a semi-dwarf phenotype with reduced plant height compared to control wildtype plants. Some of the disclosed semi-dwarf plants comprise at least one non-natural brachytic mutation in which the activity of a BR2 gene is reduced. Also disclosed are methods for producing a semi-dwarf corn plant using a CRISPR based genome editing system.
    Type: Application
    Filed: December 21, 2017
    Publication date: May 7, 2020
    Applicant: Monsanto Technology LLC
    Inventors: Ty J. Barten, Edward J. Cargill, Jonathan C. Lamb, Bryce Lemke, Linda A. Rymarquis, Dennis H. Yang
  • Publication number: 20170166912
    Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.
    Type: Application
    Filed: February 27, 2015
    Publication date: June 15, 2017
    Inventors: Brent Brower-Toland, Andrei Y. Kouranov, Rosemarie Kuehn, Richard J. Lawrence, Ervin D. Naggy, Linda Rymarquis, Veena Veena