Patents by Inventor Linda Castle

Linda Castle has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11236354
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having glyphosate-N-acetyltransferase (GLYAT) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, an improved specificity for glyphosate when compared to an appropriate control resulting in decreased off target acetylation of, e.g. an amino acid such as aspartate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the GLYAT sequences. Various methods of employing the GLYAT sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
    Type: Grant
    Filed: June 6, 2019
    Date of Patent: February 1, 2022
    Inventors: Ericka Bermudez, Linda A Castle, Kevin E McBride, Daniel Siehl
  • Patent number: 11015206
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.
    Type: Grant
    Filed: May 7, 2019
    Date of Patent: May 25, 2021
    Inventors: Phillip A Patten, Daniel Siehl, Yumin Tao, Henrik Albert, Ericka Bermudez, Linda A Castle, Yuxia Dong, Andrew Duncan Satterfield
  • Publication number: 20190292558
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having glyphosate-N-acetyltransferase (GLYAT) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, an improved specificity for glyphosate when compared to an appropriate control resulting in decreased off target acetylation of, e.g. an amino acid such as aspartate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the GLYAT sequences. Various methods of employing the GLYAT sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
    Type: Application
    Filed: June 6, 2019
    Publication date: September 26, 2019
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ERICKA BERMUDEZ, LINDA A. CASTLE, KEVIN E. MCBRIDE, DANIEL SIEHL
  • Publication number: 20190271001
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.
    Type: Application
    Filed: May 7, 2019
    Publication date: September 5, 2019
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: PHILLIP A. PATTEN, DANIEL SIEHL, YUMIN TAO, HENRIK ALBERT, ERICKA BERMUDEZ, LINDA A. CASTLE, YUXIA DONG, ANDREW DUNCAN SATTERFIELD
  • Patent number: 10329578
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having glyphosate-N-acetyltransferase (GLYAT) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, an improved specificity for glyphosate when compared to an appropriate control resulting in decreased off target acetylation of, e.g. an amino acid such as aspartate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the GLYAT sequences. Various methods of employing the GLYAT sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
    Type: Grant
    Filed: October 14, 2014
    Date of Patent: June 25, 2019
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Ericka Bermudez, Linda A. Castle, Kevin E. McBride, Daniel Siehl
  • Patent number: 10316326
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.
    Type: Grant
    Filed: May 2, 2015
    Date of Patent: June 11, 2019
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Daniel Siehl, Phillip A. Patten, Yumin Tao, Ericka Bermudez, Linda A. Castle, Yuxia Dong, Andrew Duncan Satterfield, Henrik Albert
  • Patent number: 10294487
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having ALS activity and tolerance to at least one ALS inhibitor are provided. In specific embodiments, the sequence has an increased preference for 2-ketobutyrate, when compared to an appropriate control, such as for example, HRA, and/or a preference for 2-ketobutyrate similar to a native ALS. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the ALS inhibitor tolerant sequences. Various methods of employing the ALS inhibitor tolerant sequences are provided. Such methods include methods for producing an ALS inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
    Type: Grant
    Filed: June 12, 2013
    Date of Patent: May 21, 2019
    Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Henrik Albert, John Lawrence Andreassi, II, Sean Bertain, Linda A Castle, Steven Gutteridge, Daniel L Siehl, Kay Walter
  • Patent number: 9996661
    Abstract: The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.
    Type: Grant
    Filed: December 28, 2010
    Date of Patent: June 12, 2018
    Assignee: Codexis Mayflower Holdings, LLC
    Inventors: Claes Gustafsson, Sridhar Govindarajan, Robin A. Emig, Richard John Fox, Ajoy K. Roy, Jeremy S. Minshull, S. Christopher Davis, Anthony R. Cox, Phillip A. Patten, Linda A. Castle, Daniel L. Siehl, Rebecca Lynne Gorton, Teddy Chen
  • Publication number: 20170058291
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.
    Type: Application
    Filed: May 2, 2015
    Publication date: March 2, 2017
    Inventors: Daniel Siehl, Phillip A. Patten, Yumin Tao, Ericka Bermudez, Linda A. Castle, Yuxia Dong, Andrew Duncan Satterfield, Henrik Albert
  • Publication number: 20160257971
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having glyphosate-N-acetyltransferase (GLYAT) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, an improved specificity for glyphosate when compared to an appropriate control resulting in decreased off target acetylation of, e.g. an amino acid such as aspartate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the GLYAT sequences. Various methods of employing the GLYAT sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
    Type: Application
    Filed: October 14, 2014
    Publication date: September 8, 2016
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Ericka Bermudez, Linda A. Castle, Kevin E. McBride, Daniel Siehl
  • Publication number: 20160053277
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having dicamba decarboxylase activity are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the dicamba decarboxylase sequences. Various methods of employing the dicamba decarboxylase sequences are provided. Such methods include, for example, methods for decarboxylating an auxin-analog, method for producing an auxin-analog tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional dicamba decarboxylase variants.
    Type: Application
    Filed: March 14, 2014
    Publication date: February 25, 2016
    Inventors: Eric Althoff, Yih-En Andrew Ban, Linda A. Castle, Daniela Grabs, Jian Lu, Phillip A. Patten, Yumin Tao, Alexandre Zanghellini
  • Publication number: 20160040149
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having dicamba decarboxylase activity are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the dicamba decarboxylase sequences. Various methods of employing the dicamba decarboxylase sequences are provided. Such methods include, for example, methods for decarboxylating an auxin-analog, method for producing an auxin-analog tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional dicamba decarboxylase variants.
    Type: Application
    Filed: March 14, 2014
    Publication date: February 11, 2016
    Inventors: Eric Althoff, Yi-En Andrew Ban, Linda A. Castle, Daniela Grabs, Jian Lu, Phillip A. Patten, Yumin Tao, Alexandre Zanghellini
  • Publication number: 20160017351
    Abstract: Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO: 1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof.
    Type: Application
    Filed: August 25, 2015
    Publication date: January 21, 2016
    Inventors: Henrik Albert, Linda A. Castle, Matthew Heckert, Jian Lu, Daniel L. Siehl, Yumin Tao
  • Publication number: 20150351390
    Abstract: Compositions and methods are provided to detoxify an auxin-analog herbicide through the use of at least one GH3 polypeptide having amino acid/auxin analog herbicide conjugation activity. Such GH3 polypeptides in the presence of an auxin-analog herbicide will produce an amino acid/auxin-analog conjugate having reduced herbicidal activity. Various methods of employing the GH3 polypeptides and the polynucleotides encoding the same are provided. Such methods include methods to detoxify an auxin-analog herbicide comprising applying to a plant, a plant cell or a seed an auxin-analog herbicide, wherein the plant, plant cell or seed comprises a heterologous polynucleotide encoding a GH3 polypeptide, and expression of the GH3 polypeptide produces a non-herbicidal aspartate/auxin-analog conjugate or a glutamate/auxin-analog conjugate.
    Type: Application
    Filed: December 20, 2013
    Publication date: December 10, 2015
    Inventors: Linda A. Castle, Li-Wei Chiu, Yumin Tao
  • Patent number: 9187762
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.
    Type: Grant
    Filed: August 12, 2011
    Date of Patent: November 17, 2015
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Henrik Albert, Ericka R. Bermudez, Linda A. Castle, Yuxia Dong, Matthew J. Heckert, Jingtong Hou, Zhenglin Hou, Jian Lu, Daniel L. Siehl, Yumin Tao
  • Publication number: 20150275190
    Abstract: Compositions and methods comprising promoters from the 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.
    Type: Application
    Filed: February 24, 2015
    Publication date: October 1, 2015
    Inventors: Henrik Albert, Linda A. Castle, Jian Lu, Yumin Tao
  • Patent number: 9139842
    Abstract: Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof.
    Type: Grant
    Filed: August 12, 2011
    Date of Patent: September 22, 2015
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Henrik Albert, Linda A. Castle, Matthew Heckert, Jian Lu, Daniel L. Siehl, Yumin Tao
  • Patent number: 8993837
    Abstract: Compositions and methods comprising promoters from the 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.
    Type: Grant
    Filed: August 12, 2011
    Date of Patent: March 31, 2015
    Assignee: Pioneer Hi-Bred International, Inc
    Inventors: Henrik Albert, Linda A. Castle, Jian Lu, Yumin Tao
  • Patent number: 8962328
    Abstract: Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.
    Type: Grant
    Filed: April 3, 2012
    Date of Patent: February 24, 2015
    Assignees: Pioneer Bred International Inc, E.I. DuPont de Nemours
    Inventors: Hyeon-Je Cho, Linda A. Castle, Theodore M. Klein, Tai Minh, York Moy
  • Patent number: 8849575
    Abstract: The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.
    Type: Grant
    Filed: May 5, 2006
    Date of Patent: September 30, 2014
    Assignee: Codexis Mayflower Holdings, LLC
    Inventors: Claes Gustafsson, Sridhar Govindarajan, Robin A. Emig, Richard John Fox, Ajoy K. Roy, Jeremy S. Minshull, S. Christopher Davis, Anthony R. Cox, Phillip A. Patten, Linda A. Castle, Daniel L. Siehl, Rebecca Lynne Gorton, Teddy Chen