Abstract: The present invention relates to attenuated African Swine Fever Viruses. The attenuated viruses protect pigs against subsequent challenge with virulent virus. The present invention also relates to the use of such attenuated viruses to treat and/or prevent African Swine Fever.

Abstract: The present invention relates to attenuated African Swine Fever viruses. The attenuated viruses protect pigs against subsequent challenge with virulent virus. The present invention also relates to the use of such attenuated viruses to treat and/or prevent African Swine Fever.

Abstract: The present invention provides an African swine fever virus (ASFV) subunit vaccine which comprises: (i) one or more recombinant polynucleotides which encode polypeptides shown as SEQ ID NO: 1, 2 and 3 or an immunogenic fragment thereof; or a variant with at least 70% sequence identity to one of SEQ ID NO: 1, 2 or 3; wherein the total number of different ASFV polypeptides encoded by the one or more recombinant polynucleotides is 10 or fewer; or (ii) recombinant polypeptides shown as SEQ ID NO: 1, 2 and 3 or an immunogenic fragment thereof; or a variant with at least 70% sequence identity to one of SEQ ID NO: 1, 2 and 3; wherein vaccine comprises 10 or fewer different ASFV polypeptides.

Abstract: The present invention provides an attenuated African Swine Fever (ASF) virus which lacks a functional version of the following genes: multigene-family 360 genes 9L, 10L, 11L, 12L, 13L and 14L; and multigene-family 505 genes 1R, 2R, 3R and 4R. The invention further provides an attenuated African Swine Fever (ASF) virus which Lacks a functional version of the DP148R gene. The present invention also provides a vaccine comprising such an attenuated virus and its use to prevent ASF. Further, the invention relates to intranasal administration of an attenuated ASF virus.

Abstract: The present invention provides an attenuated African Swine Fever (ASF) virus which lacks a functional version of the following genes: multigene-family 360 genes 9L, 10L, 11L, 12L, 13L and 14L; and multigene-family 505 genes 1R, 2R, 3R and 4R. The invention further provides an attenuated African Swine Fever (ASF) virus which Lacks a functional version of the DP148R gene. The present invention also provides a vaccine comprising such an attenuated virus and its use to prevent ASF. Further, the invention relates to intranasal administration of an attenuated ASF virus.

Abstract: The present invention provides an attenuated African Swine Fever (ASF) virus which lacks a functional version of the following genes: multigene-family 360 genes 9L, 10L, 11L, 12L, 13L and 14L; and multigene-family 505 genes 1R, 2R, 3R and 4R. The invention further provides an attenuated African Swine Fever (ASF) virus which lacks a functional version of the DP148R gene. The present invention also provides a vaccine comprising such an attenuated virus and its use to prevent ASF. Further, the invention relates to intranasal administration of an attenuated ASF virus.

Abstract: The present invention provides an attenuated African Swine Fever (ASF) virus which lacks a functional version of the following genes: multigene-family 360 genes 9L, 10L, 11L, 12L, 13L and 14L; and multigene-family 505 genes 1R, 2R, 3R and 4R. The invention further provides an attenuated African Swine Fever (ASF) virus which lacks a functional version of the DP148R gene. The present invention also provides a vaccine comprising such an attenuated virus and its use to prevent ASF. Further, the invention relates to intranasal administration of an attenuated ASF virus.

Abstract: A needle device having a plurality of needles with applied tips arranged so as to define a plurality of contact edges. In one embodiment one or more oblique edges is provided relative to an intermediate horizontal edge or a plurality of oblique edges with adjacent edges sharing a common needle point vertex. The number and needle grading (e.g., fine/sharper tips; medium and heavy grades) are represented by the different contact edges of the needle device. The number of each needle sub-sets is also preferably varied to provide enhanced flexibility as the application as in cosmetic tattooing. A tool for supporting is also preferably provided as in a reciprocating needle device support is provided. The needle device is particularly well suited for providing permanent eye liner makeup in view of its adaptability to accommodate different skin textures and skin surface presentations.

Abstract: A plurality of different intradermal needle penetration application techniques, preferably both of a powered and non-powered application type, are featured that enable a wide variety of applications with preferably only a single needle device that includes a plurality of needles with preferably a single tip slope edge arranged at a gentle slant, preferably a 30-35° slant, or a plurality of such sloped needle tip edges. The needle arrangement enables the needle set to enter at the same time the skin of the subject to an equal desired depth in consistent fashion. The same needle device can be used for point marking, lining and shading with ease. The application processes include use of a plurality of the provided application techniques preferably in a favored order of application and intermixing the point, lining and/or shading techniques to achieve a highly efficient and rapid application process.

Abstract: A needle device having a plurality of needles with applied tips arranged so as to define a plurality of contact edges. In one embodiment one or more oblique edges is provided relative to an intermediate horizontal edge or a plurality of oblique edges with adjacent edges sharing a common needle point vertex. The number and needle grading (e.g., fine/sharper tips; medium and heavy grades) are represented by the different contact edges of the needle device. The number of each needle sub-sets is also preferably varied to provide enhanced flexibility as the application as in cosmetic tattooing. A tool for supporting is also preferably provided as in a reciprocating needle device support is provided. The needle device is particularly well suited for providing permanent eye liner makeup in view of its adaptability to accommodate different skin textures and skin surface presentations.