Abstract: A dental floss dispenser is designed to retain a roll of dental floss which may be dispensed and cut through an opening. The floss is provided in preselected lengths interconnected by shorter lengths of thread. Once dispensed and cut in a medial portion of the thread, the threaded ends of a length of floss may be held to use the floss which prevents any wastage of flossing material. In modified embodiments, the lengths of thread may be provided with small knots to indicate where to cut the thread and to provide a better grip during a use of the floss. Further, granules of fluoride may be attached to a floss strip to act both as an abrasive and as a means of applying fluoride between teeth as the granules dissolve.