Abstract: The present invention is directed to a combination of two types of biocidal materials that give rise to synergistic effects against microorganisms in aqueous systems such that the extent to which the microorganisms are killed exceeds that which one would expect from using the sum of the individual components of the composition. More specifically, this invention is directed to a combination comprising 2-(decylthio)ethaneamine and 1,2-dibromo-2,4-dicyanobutane in an amount effective to inhibit microbial growth. This combination has proven to be particularly effective in inhibiting the growth of Enterobacter aerogenes ATCC 13048 in aqueous systems.

Abstract: After baking bread dough goods in an oven at about 375.degree. F., their temperature is lowered to at least about 120.degree. F., and they are then cryogenically cooled to about 45.degree. F. or below in about 5 to about 15 minutes. The baked goods are then maintained at a temperature of about 37.degree. F. to about 45.degree. F. for at least about 25 minutes to effect substantial starch crystallization; after which time, slicing, bagging and freezing are carried out. Cryogenic cooling is preferably effected in a CO.sub.2 cooler, and the cooled baked goods are maintained at about 40.degree. F. for between about 25 and about 60 minutes prior to freezing by movement along an insulated conveyor which is cooled by circulation therethrough of cold CO.sub.2 vapor exhaust from the CO.sub.2 cooler.