Abstract: The present disclosure relates to the technical field of display panels and provides a driving method and a driving system of a display panel. The driving method of a display panel includes detecting a refresh rate of each frame of the display panel; in response to that the refresh rate of a current frame of the display panel changes relative to the refresh rate of a previous frame of the current frame, generating an adjustment instruction of an initialization voltage; determining a target initialization voltage of the current frame based on the adjustment instruction of the initialization voltage; and at a reset phase of the current frame, initializing a pixel drive circuit of the display panel with the target initialization voltage, so that a difference value between a working current of the pixel drive circuit in the current frame and a working current of the pixel drive circuit in the previous frame is less than a preset threshold.