Abstract: A microfluidic device, including: a main body, a sealing layer and a plurality of Polymerase Chain Reaction (PCR) units arranged on the main body. Each PCR unit includes a microchannel arranged on a surface of the main body, and a sample reservoir, an oil reservoir, a droplet generation zone, a transition zone, a droplet storage zone and a collection zone that are communicated with each other through the microchannel. The sealing layer is arranged on the surface of the main body to seal the main body. After generated, the droplets are collected in the oil reservoir and then distributed to the droplet storage zone through the transition zone.

Abstract: Disclosed is a microfluidic chip, including a chip upper cover, a chip lower layer, a membrane, a sealing gasket and a sealing ring. The microfluidic chip is provided with a sample storage zone, a droplet formation zone, a droplet storage zone, a droplet detection zone and a waste liquid storage zone. The sample storage zone, the droplet formation zone, the droplet storage zone, the droplet detection zone and the waste liquid storage zone communicate by means of a micropore or a micro-channel. The droplet formation zone is used to transform the sample phase into tens of thousands to millions of droplets, the droplets undergo the PCR reaction in the droplet storage zone, the droplet detection zone is used to perform optical detection on the droplets after PCR reaction, and the waste liquid storage zone is used to collect and store the detected droplets and continuous phase.

Abstract: A microfluidic device, including: a main body, a sealing layer and a plurality of Polymerase Chain Reaction (PCR) units arranged on the main body. Each PCR unit includes a microchannel arranged on a surface of the main body, and a sample reservoir, an oil reservoir, a droplet generation zone, a transition zone, a droplet storage zone and a collection zone that are communicated with each other through the microchannel. The sealing layer is arranged on the surface of the main body to seal the main body. After generated, the droplets are collected in the oil reservoir and then distributed to the droplet storage zone through the transition zone.

Abstract: Disclosed is a microfluidic chip, including a chip upper cover, a chip lower layer, a membrane, a sealing gasket and a sealing ring. The microfluidic chip is provided with a sample storage zone, a droplet formation zone, a droplet storage zone, a droplet detection zone and a waste liquid storage zone. The sample storage zone, the droplet formation zone, the droplet storage zone, the droplet detection zone and the waste liquid storage zone communicate by means of a micropore or a micro-channel. The droplet formation zone is used to transform the sample phase into tens of thousands to millions of droplets, the droplets undergo the PCR reaction in the droplet storage zone, the droplet detection zone is used to perform optical detection on the droplets after PCR reaction, and the waste liquid storage zone is used to collect and store the detected droplets and continuous phase.

Abstract: A microfluidic chip includes a chip upper layer, a chip lower layer, a sealing layer, and a droplet generation area, a droplet storage area, a droplet detection area and a waste liquid collection area that are provided on the microfluidic chip and that are communicated to each other through a channel. The droplet generation area is used for producing tens of thousands to millions of droplets by passing a sample phase through a continuous phase, after the droplets enter the droplet storage area for undergoing a PCR reaction, the droplet detection area is used for the optical detection of the droplet having undergone the PCR reaction, and the waste liquid collection area is used for collecting and storing the droplets and the continuous phase after detection.

Abstract: Methods of isolating at least one cell of interest, methods of making fixed arrays, arrays comprising a glass substrate bonded to a patterned siloxane structure having inlets, outlets and microchannels, array kits, and methods of making microfluidic apparati are provided in the present application.