Abstract: A microfluidic device and method comprises a rotatable substrate configured to be rotated at various angular velocities in order to accomplish a series of media exchanges with a sample chamber for containing a sample. A plurality of media reservoirs and waste reservoirs are connected to the sample chamber via channels forming capillary valves in which the capillary valves have varying burst frequencies of rotation of the substrate. The substrate can then be rotated through a series of angular velocities to automate media exchanges between the sample chamber and the respective media reservoirs and waste reservoirs. A pair of media reservoirs are located radially inward of the sample chamber and a pair of waste reservoirs are located radially outward of the sample chamber in an “X” configuration such that centrifugal forces cause fluid media to open the capillary valves and flow among the sample chamber, media reservoirs and waste reservoirs.

Abstract: A method for enhancing sauce flavor including steps of: collection, dilution, denaturation, enzymolysis, enzyme deactivation, centrifugation, secondary enzymolysis, deodorization, Maillard reaction, seasoning, dosing and flexible sterilization of a raw material, salted duck egg white. The by-product, salted duck egg white, not fully used in the food processing industry is recycled as a raw material, which not only saves the cost but also can protect the environment. Hydrolyzed animal protein is obtained through double-enzyme fractional hydrolysis. The degree of hydrolysis is high. The obtained hydrolyzed protein contains no trichloropropanol and is relatively safe, and flavourzyme decomposes bitter peptide, to make the hydrolysate not taste bitter. Some yeast, cooking wine and essence with unique flavor are chosen. Each ingredient is in a reasonable proportion, and the obtained product is delicious and of mellow flavor.

Abstract: A microfluidic device and method comprises a rotatable substrate configured to be rotated at various angular velocities in order to accomplish a series of media exchanges with a sample chamber for containing a sample. A plurality of media reservoirs and waste reservoirs are connected to the sample chamber via channels forming capillary valves in which the capillary valves have varying burst frequencies of rotation of the substrate. The substrate can then be rotated through a series of angular velocities to automate media exchanges between the sample chamber and the respective media reservoirs and waste reservoirs. A pair of media reservoirs are located radially inward of the sample chamber and a pair of waste reservoirs are located radially outward of the sample chamber in an “X” configuration such that centrifugal forces cause fluid media to open the capillary valves and flow among the sample chamber, media reservoirs and waste reservoirs.

Abstract: Novel chemopreventive and chemotherapeutic cancer treatment method using elemental selenium nanoparticles. Cancer cells, especially androgen dependent prostate cancers are exposed to selenium from elemental selenium nanoparticle treatment, and apoptosis is induced in the cancer cells.