Abstract: The present invention provides a method for plant genome modification, preferably for the targeted modification of at least one genomic target sequence, for obtaining at least one modified cell, wherein modification of a cell is achieved by providing a genome modification or editing system together with at least one regeneration booster, or a combination of regeneration boosters, which is/are transiently active in the cell and/or with at least one epigenetically regulating chemical. Preferably, the effector molecules are introduced by means of particle bombardment. Furthermore, the modified plant cell is regenerated to obtain a plant, which inherits the modification to its progeny. In addition, methods, tools, constructs and strategies are provided to effectively modify the genome of a plant cell or at least one genomic target site in a plant cell, to obtain said modified cell and to regenerate a plant tissue, organ, plant or seed from the modified cell.

Abstract: The present invention relates to a method for plant genome modification of at least one plant cell being in the developmental stage of a plant immature inflorescence meristem (IIM) cell, wherein the modification of the specific cell type is achieved by providing a genome modification or editing system, optionally together with at least one regeneration booster, preferably wherein the effector molecules are introduced by means of particle bombardment. To this end, new artificial and precisely controllable booster genes and proteins are provided. Further, the modified plant cells are regenerated in a direct or an indirect way. Finally, methods, tools, constructs and strategies are provided to effectively modify at least one genomic target site in a plant cell, to obtain said modified cell and to regenerate a, plant tissue, organ, plant or seed from such modified cell.

Abstract: Disclosed is an intelligent pollen monitoring and analysis system and control method. The system includes a monitoring box equipped with a control module and a support platform. The support platform is equipped with a slide storage rack, a pollen sampling assembly, an image acquisition device, and a slide transfer mechanism. The slide storage rack is arranged on a rotating mechanism, and the image acquisition device is equipped with a fine-tuning mechanism. The rotating mechanism, the pollen sampling assembly, the image acquisition device, the slide transfer mechanism, and the fine-tuning mechanism are connected to the control module. The control module is used to control the start and stop of the components, and recognize the pollen concentration and/or pollen quantity uploaded by the image acquisition device. This solution solves the problem of inaccurate pollen concentration monitoring caused by vibration during pollen transmission using sampling tape strips in existing pollen monitoring devices.

Abstract: Disclosed is an intelligent pollen monitoring and analysis system and control method. The system includes a monitoring box equipped with a control module and a support platform. The support platform is equipped with a slide storage rack, a pollen sampling assembly, an image acquisition device, and a slide transfer mechanism. The slide storage rack is arranged on a rotating mechanism, and the image acquisition device is equipped with a fine-tuning mechanism. The rotating mechanism, the pollen sampling assembly, the image acquisition device, the slide transfer mechanism, and the fine-tuning mechanism are connected to the control module. The control module is used to control the start and stop of the components, and recognize the pollen concentration and/or pollen quantity uploaded by the image acquisition device. This solution solves the problem of inaccurate pollen concentration monitoring caused by vibration during pollen transmission using sampling tape strips in existing pollen monitoring devices.

Abstract: The present invention relates to a method for plant genome modification of at least one plant cell being in the developmental stage of a plant immature inflorescence meristem (IIM) cell, wherein the modification of the specific cell type is achieved by providing a genome modification or editing system, optionally together with at least one regeneration booster, preferably wherein the effector molecules are introduced by particle bombardment. To this end, new artificial and precisely controllable booster genes and proteins are provided. Further, the modified plant cells are regenerated in a direct or an indirect way. Finally, methods, tools, constructs and strategies are provided to effectively modify at least one genomic target site in a plant cell, to obtain the modified plant cell and to regenerate a plant tissue, organ, plant or seed from the modified plant cell.

Abstract: This document relates to methods and materials for genome engineering in eukaryotic cells, and particularly to methods for increasing genome engineering (i.e. transformation or genome editing) efficiency via delivery of one or more booster polypeptides, and boost genes, with genome engineering components.

Abstract: This document relates to methods and materials for genome engineering in eukaryotic cells, and particularly to methods for increasing genome engineering (i.e. transformation or genome editing) efficiency via co-delivery of combinations of one or more booster polypeptides, and boost genes, with genome engineering components.

Abstract: This document relates to methods and materials for genome engineering in eukaryotic cells, and particularly to methods for increasing genome engineering (i.e. transformation or genome editing) efficiency via delivery of one or more booster polypeptides, and boost genes, with genome engineering components.

Abstract: The present invention provides a method for plant genome modification, preferably for the targeted modification of at least one genomic target sequence, for obtaining at least one modified cell, wherein modification of a cell is achieved by providing a genome modification or editing system together with at least one regeneration booster, or a combination of regeneration boosters, which is/are transiently active in the cell and/or with at least one epigenetically regulating chemical. Preferably, the effector molecules are introduced by means of particle bombardment. Furthermore, the modified plant cell is regenerated to obtain a plant, which inherits the modification to its progeny. In addition, methods, tools, constructs and strategies are provided to effectively modify the genome of a plant cell or at least one genomic target site in a plant cell, to obtain said modified cell and to regenerate a plant tissue, organ, plant or seed from the modified cell.

Abstract: A one-step molded lithium ion battery separator and preparation method and application thereof are provided. The battery separator comprises a support layer and a filler layer. The support layer comprises at least two of superfine main fiber, thermoplastic bonded fiber and first nanofiber, and the filler layer comprises at least one of inorganic fillers and third nanofiber. The lithium ion battery separator has a thickness of 19-31 ?m, a maximum pore diameter of no more than 1 ?m, and a heat shrinkage rate of less than 3% after treatment at 300° C. for 1 hour, and the separator still has a certain strength at a high temperature, ensuring stability and isolation of the rigid structure of the filler layer at a high temperature, satisfying requirements of the separator in terms of heat resistance, pore size and strength, having excellent comprehensive performance.

Abstract: A battery separator and a preparation method therefor are provided. The separator includes a lithium ion battery separator substrate and an inorganic coating, the lithium ion battery separator substrate consists of a support layer and a dense layer, and the inorganic coating is coated on the dense layer; the separator has excellent high-temperature resistance, and still has good strength retention and the heat shrinkage rate thereof is no more than 2% after treatment at 300° C. for 1 h, and thus ensures the stability and isolation of the rigid structure of the separator coating at high temperatures; the substrate has a uniform and compact double-layer structure, effectively controls phenomena such as pinholes and filler particles fall-off in a subsequent coating process, and meets the requirements of lithium ion battery separators with respect to heat resistance, porosity and strength, thus having excellent comprehensive performance.

Abstract: A lithium ion battery separator substrate, a preparation method and application thereof are provided. The substrate comprises a support layer and a dense layer, wherein the support layer comprises superfine main fibers, thermoplastic bonded fibers and the nanofibers, and the dense layer comprises nanofibers. The substrate has excellent high-temperature resistance performance, the substrate still has certain strength after being processed at 300° C. for 1 h, and the heat shrinkage rate is less than 5.0%; the substrate has a uniform and compact double-layer structure without a pinhole. Therefore, the requirements concerning heat resistance, porosity and strength of the substrate are met.

Abstract: The present invention relates to a method for plant genome modification of at least one plant cell being in the developmental stage of a plant immature inflorescence meristem (IIM) cell, wherein the modification of the specific cell type is achieved by providing a genome modification or editing system, optionally together with at least one regeneration booster, preferably wherein the effector molecules are introduced by means of particle bombardment. To this end, new artificial and precisely controllable booster genes and proteins are provided. Further, the modified plant cells are regenerated in a direct or an indirect way. Finally, methods, tools, constructs and strategies are provided to effectively modify at least one genomic target site in a plant cell, to obtain said modified cell and to regenerate a, plant tissue, organ, plant or seed from such modified cell.

Abstract: A method for modifying a plant includes coating a microparticle with at least one type of nucleic acid and/or at least one type of a protein, bombarding a shoot apex of a plant with the coated microparticle, growing the shoot apex bombarded with the coated microparticle to obtain a plant body, and selecting a modified plant body from the plant body. The shoot apex is selected from the group consisting of a shoot apex of an embryo of a fully mature seed, a shoot apex of a young bud, a shoot apex of a terminal bud or a lateral bud, and a shoot apex of an immature embryo.

Abstract: This document relates to methods and materials for genome engineering in eukaryotic cells, and particularly to methods for increasing genome engineering (i.e. transformation or genome editing) efficiency via delivery of one or more booster polypeptides, and boost genes, with genome engineering components.

Abstract: This document relates to methods and materials for genome engineering in eukaryotic cells, and particularly to methods for increasing genome engineering (i.e. transformation or genome editing) efficiency via co-delivery of combinations of one or more booster polypeptides, and boost genes, with genome engineering components.

Abstract: This document relates to methods and materials for genome engineering in eukaryotic cells, and particularly to methods for increasing genome engineering (i.e. transformation or genome editing) efficiency via co-delivery of one or more chemicals, such as protein deacetylase inhibitors, phytohormones and/or regeneration boost genes, with genome engineering components.

Abstract: A lithium ion battery separator substrate, a preparation method and application thereof are provided. The substrate comprises a support layer and a dense layer, wherein the support layer comprises superfine main fibers, thermoplastic bonded fibers and the nanofibers, and the dense layer comprises nanofibers. The substrate has excellent high-temperature resistance performance, the substrate still has certain strength after being processed at 300° C. for 1 h, and the heat shrinkage rate is less than 5.0%; the substrate has a uniform and compact double-layer structure without a pinhole. Therefore, the requirements concerning heat resistance, porosity and strength of the substrate are met.

Abstract: A battery separator and a preparation method therefor are provided. The separator includes a lithium ion battery separator substrate and an inorganic coating, the lithium ion battery separator substrate consists of a support layer and a dense layer, and the inorganic coating is coated on the dense layer; the separator has excellent high-temperature resistance, and still has good strength retention and the heat shrinkage rate thereof is no more than 2% after treatment at 300° C. for 1 h, and thus ensures the stability and isolation of the rigid structure of the separator coating at high temperatures; the substrate has a uniform and compact double-layer structure, effectively controls phenomena such as pinholes and filler particles fall-off in a subsequent coating process, and meets the requirements of lithium ion battery separators with respect to heat resistance, porosity and strength, thus having excellent comprehensive performance.

Abstract: A one-step molded lithium ion battery separator and preparation method and application thereof are provided. The battery separator comprises a support layer and a filler layer. The support layer comprises at least two of superfine main fiber, thermoplastic bonded fiber and first nanofiber, and the filler layer comprises at least one of inorganic fillers and third nanofiber. The lithium ion battery separator has a thickness of 19-31 ?m, a maximum pore diameter of no more than 1 ?m, and a heat shrinkage rate of less than 3% after treatment at 300° C. for 1 hour, and the separator still has a certain strength at a high temperature, ensuring stability and isolation of the rigid structure of the filler layer at a high temperature, satisfying requirements of the separator in terms of heat resistance, pore size and strength, having excellent comprehensive performance.