Abstract: The present disclosure relates to a method for constructing a whole genome high-throughput sequencing library comprising the following steps: (1) extracting a sample gDNA; (2) fragmenting said sample gDNA by enzyme cleavage, filling ends of the gDNA and adding A base to the gDNA fragments to obtain an A-added gDNA; (3) connecting the A-added gDNA with a linker combination to obtain a connected produce, said linker combination comprises two parts: a Y-shaped reverse linker and a high GC clamp linker; (4) purifying said connected product to obtain a purified product; and (5) screening the fragment of said purified product to obtain a sequencing library. The present disclosure also relates to a kit for constructing a whole genome high-throughput sequencing library.

Abstract: Provided herein is a method of constructing a high throughput sequencing library for use in detecting chromosome copy number variation comprising mainly the following steps: (1) subjecting DNAs to be tested to random fragmentation by a double-strand DNA fragmentation enzyme; (2) end-filling and adding poly-adenine at the 3?end of the fragmented DNAs; (3) connecting the end-filled DNAs having a 3?end poly-adenine with sequencing linkers to obtain connected products; (4) purifying the connected products to obtain the sequencing library; wherein steps (1)-(3) are performed in a single reaction tube. Also provided is a kit for constructing a sequencing library for use in detecting chromosome copy number variation.

Abstract: An improved balloon catheter for angioplasty and the like for measuring the inflation of a balloon after insertion into the body. A pair of electrodes are mounted in spaced relation within the balloon interior wall such that as the internal area within the balloon is varied by inflation of the balloon with an electrically conductive fluid, the electrodes monitor the changing electrical resistance between the electrodes. The electrodes are connected through the catheter to an external electrical measurement circuit for measuring the change in resistance and thus determining the amount of balloon inflation.