Patents by Inventor Lingjun Li

Lingjun Li has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210181206
    Abstract: The present invention discloses mass spectrometry (MS)-based methods and tagging reagents for qualitative and quantitative analysis of post-translational modified (PTM) proteins in biological and clinical samples. The present invention utilizes thiol-containing tagging reagents which are able to bind to or derivatize biomolecules, preferably post-translational modified (PTM) polypeptides such as citrullinated and homocitrullinated polypeptides. The tagging reagents are preferably biotin-derived tags able to react with a side chain of the modified polypeptides.
    Type: Application
    Filed: December 14, 2020
    Publication date: June 17, 2021
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Lingjun LI, Yatao SHI, Zihui LI
  • Publication number: 20210021999
    Abstract: The systems and methods described herein can include a digital assistant application that receives sensor signals from sensors installed in a vehicle and determines an entry event into the vehicle. The digital assistant application can receive, responsive to the entry event into the vehicle, a plurality authentication input signals from a plurality of sensors associated with the vehicle. The digital assistant application can determine a plurality of authentication states based on the plurality of authentication input signals and a plurality of authentication credentials. The digital assistant application can identify an access permission level of a plurality of access permission levels based at least in part on the plurality of identifies authentication states. The digital assistant application can identify, responsive to the access permission level, a subset of a set of functionalities available via the vehicle, and provide vehicular access to the subset of functionalities.
    Type: Application
    Filed: October 6, 2020
    Publication date: January 21, 2021
    Inventors: Haris Ramic, Victor Chan, Vikas Gupta, Lingjun Li
  • Publication number: 20200392550
    Abstract: Disclosed herein are compositions, methods, cells, engineered microorganisms, and kits for increasing the production of proteins or polypeptides comprising one or more unnatural amino acids. Further provided are compositions, cells, engineered microorganisms, and kits for increasing the retention of unnatural nucleic acids encoding the unnatural amino acids in an engineered cell, or semi-synthetic organism.
    Type: Application
    Filed: June 12, 2020
    Publication date: December 17, 2020
    Inventors: Floyd E. ROMESBERG, Vivian T. DIEN, Aaron W. FELDMAN, Rebekah J. KARADEEMA, Lingjun LI, Michael P. LEDBETTER, Anne Xiaozhou ZHOU
  • Publication number: 20200361952
    Abstract: The present invention provides a compound having a structure of formula (I), a preparation method and use thereof, and a pharmaceutical composition containing the compound, wherein R is methyl, ethyl, propyl, vinyl or propenyl. The present invention also provides a crystalline form of the compound, a preparation method and use of the crystalline form, and a pharmaceutical composition comprising the crystalline form. The compounds having the structure of formula (I) of the present invention are present in a solid form, which not only can solve the problem of clopidogrel resistance, but also can solve the problem of severe hemorrhagic side effect and poor safety of some drugs, as well as the problem of poor stability of existing compounds. It can be developed into an ADP receptor antagonist antiplatelet agent with clear therapeutic effect, no resistance and better stability.
    Type: Application
    Filed: August 22, 2018
    Publication date: November 19, 2020
    Applicant: Tianjin Institute of Pharmaceutical Research Co., Ltd.
    Inventors: Changjiang Huang, Shijun Zhang, Lingjun Li, Lei Liu, Yuquan Li, Jing Yuan, Hui Yan, Songhui Wang, Xuemin Zheng, Qunchao Wei, Xuyuan Liu, Wei Wei, Weiren Xu, Lida Tang, Meixiang Zou
  • Patent number: 10812976
    Abstract: The systems and methods described herein can include a digital assistant application that receives sensor signals from sensors installed in a vehicle and determines an entry event into the vehicle. The digital assistant application can receive, responsive to the entry event into the vehicle, a plurality authentication input signals from a plurality of sensors associated with the vehicle. The digital assistant application can determine a plurality of authentication states based on the plurality of authentication input signals and a plurality of authentication credentials. The digital assistant application can identify an access permission level of a plurality of access permission levels based at least in part on the plurality of identifies authentication states. The digital assistant application can identify, responsive to the access permission level, a subset of a set of functionalities available via the vehicle, and provide vehicular access to the subset of functionalities.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: October 20, 2020
    Assignee: GOOGLE LLC
    Inventors: Haris Ramic, Victor Chan, Vikas Gupta, Lingjun Li
  • Patent number: 10626138
    Abstract: Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
    Type: Grant
    Filed: August 8, 2014
    Date of Patent: April 21, 2020
    Assignees: THE SCRIPPS RESEARCH INSTITUTE NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT NIH DIVISION OF EXTRAMURAL INVENTIONS AND TECHNOLOGY RESOURCES (DEITR)
    Inventors: Floyd E. Romesberg, Denis A. Malyshev, Lingjun Li, Thomas Lavergne, Zhengtao Li
  • Publication number: 20200068400
    Abstract: The systems and methods described herein can include a digital assistant application that receives sensor signals from sensors installed in a vehicle and determines an entry event into the vehicle. The digital assistant application can receive, responsive to the entry event into the vehicle, a plurality authentication input signals from a plurality of sensors associated with the vehicle. The digital assistant application can determine a plurality of authentication states based on the plurality of authentication input signals and a plurality of authentication credentials. The digital assistant application can identify an access permission level of a plurality of access permission levels based at least in part on the plurality of identifies authentication states. The digital assistant application can identify, responsive to the access permission level, a subset of a set of functionalities available via the vehicle, and provide vehicular access to the subset of functionalities.
    Type: Application
    Filed: October 3, 2017
    Publication date: February 27, 2020
    Inventors: Haris Ramic, Victor Chan, Vikas Gupta, Lingjun Li
  • Publication number: 20200040027
    Abstract: Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
    Type: Application
    Filed: July 22, 2019
    Publication date: February 6, 2020
    Inventors: Floyd E. ROMESBERG, Denis A. MALYSHEV, Lingjun LI, Thomas LAVERGNE, Zhengtao LI
  • Publication number: 20200017540
    Abstract: Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
    Type: Application
    Filed: August 8, 2019
    Publication date: January 16, 2020
    Inventors: Floyd E. ROMESBERG, Denis A. MALYSHEV, Lingjun LI, Thomas LAVERGNE, Zhengtao LI
  • Publication number: 20190225559
    Abstract: The present invention provides a set of novel isobaric chemical tags, also referred herein as SUGAR (Isobaric Multiplex Reagents for Carbonyl Containing Compound). These labeling tags are compact and easy to synthesize at high yield and purity in just a few steps using commercially available starting materials. The tagging reagents of the present invention comprise: a) a reporter group, having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an aldehyde, ketone, or carboxylic acid reactive group. The multiplex SUGAR tags are able to react with an aldehyde, ketone, or carboxylic acid group of the molecule to be tagged, which offers the capability for labeling and quantitation of glycans, proteins/peptides, and fatty acids.
    Type: Application
    Filed: January 18, 2019
    Publication date: July 25, 2019
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Lingjun LI, Yu FENG, Bingming CHEN, Qinying YU, Xuefei ZHONG, Miyang LI
  • Patent number: 10119974
    Abstract: Compositions and methods of tagging peptides and other molecules using novel isobaric tandem mass tagging reagents, including novel N, N-dimethylated amino acid 8-plex and 16-plex isobaric tandem mass tagging reagents. The tagging reagents comprise: a) a reporter group having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an amine reactive group. The tagging reagents disclosed herein serve as attractive alternatives for isobaric tag for relative and absolute quantitation (iTRAQ) and tandem mass tags (TMTs) due to their synthetic simplicity, labeling efficiency and improved fragmentation efficiency.
    Type: Grant
    Filed: June 14, 2016
    Date of Patent: November 6, 2018
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Lingjun Li, Feng Xiang, Tyler Greer, Dustin Frost, Zhidan Liang
  • Publication number: 20180299464
    Abstract: The use of mass defect signatures to impart milliDalton mass differences between isotopically labeled peptides at the MS1-level allows multiplex quantification without the increased mass spectral complexity that occurs with mass difference approaches. Provided herein is a mass defect-based chemical tag, dimethyl pyrimidinyl ornithine (DiPyrO), that is compact and easy to synthesize at high purity in few steps using commercially available starting materials. The multiplex DiPyrO tags are amine-reactive and can impart a mass difference onto labeled peptides and through calculated substitution of heavy isotopes. DiPyrO offers up to 10-plex quantification on current Orbitrap or FT-ICR platforms without increasing mass spectral complexity. The synthesis of the DiPyrO tag is provided along with viability of the DiPyrO tag for labeling complex proteomics samples using yeast extract digests and its effect on labeled peptides during LC-MS2 analysis.
    Type: Application
    Filed: October 14, 2016
    Publication date: October 18, 2018
    Inventors: Lingjun LI, Dustin FROST, Amanda BUCHBERGER
  • Patent number: 9996803
    Abstract: Systems and methods for authenticating a user through an unobservable re-authentication process are disclosed.
    Type: Grant
    Filed: September 3, 2014
    Date of Patent: June 12, 2018
    Assignee: Arizona Board of Regents on Behalf of Arizona State University
    Inventors: Lingjun Li, Xinxin Zhao, Guoliang Xue
  • Publication number: 20160356786
    Abstract: Compositions and methods of tagging peptides and other molecules using novel isobaric tandem mass tagging reagents, including novel N, N-dimethylated amino acid 8-plex and 16-plex isobaric tandem mass tagging reagents. The tagging reagents comprise: a) a reporter group having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an amine reactive group. The tagging reagents disclosed herein serve as attractive alternatives for isobaric tag for relative and absolute quantitation (iTRAQ) and tandem mass tags (TMTs) due to their synthetic simplicity, labeling efficiency and improved fragmentation efficiency.
    Type: Application
    Filed: June 14, 2016
    Publication date: December 8, 2016
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Lingjun LI, Feng XIANG, Tyler GREER, Dustin FROST, Zhidan LIANG
  • Patent number: 9388132
    Abstract: Compositions and methods of tagging peptides and other molecules using novel isobaric tandem mass tagging reagents, including novel N,N-dimethylated amino acid 8-plex and 16-plex isobaric tandem mass tagging reagents. The tagging reagents comprise: a) a reporter group having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an amine reactive group. The tagging reagents disclosed herein serve as attractive alternatives for isobaric tag for relative and absolute quantitation (iTRAQ) and tandem mass tags (TMTs) due to their synthetic simplicity, labeling efficiency and improved fragmentation efficiency.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: July 12, 2016
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Lingjun Li, Feng Xiang, Tyler Greer, Dustin Frost, Zhidan Liang
  • Publication number: 20160168187
    Abstract: Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
    Type: Application
    Filed: August 8, 2014
    Publication date: June 16, 2016
    Applicant: Synthorx, Inc.
    Inventors: Floyd E. ROMESBERG, Denis A. MALYSHEV, Lingjun LI, Thomas LAVERGNE, Zhengtao LI
  • Publication number: 20150066822
    Abstract: Systems and methods for authenticating a user through an unobservable re-authentication process are disclosed.
    Type: Application
    Filed: September 3, 2014
    Publication date: March 5, 2015
    Inventors: Lingjun Li, Xinxin Zhao, Guoliang Xue
  • Publication number: 20130078728
    Abstract: Compositions and methods of tagging peptides and other molecules using novel isobaric tandem mass tagging reagents, including novel N,N-dimethylated amino acid 8-plex and 16-plex isobaric tandem mass tagging reagents. The tagging reagents comprise: a) a reporter group having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an amine reactive group. The tagging reagents disclosed herein serve as attractive alternatives for isobaric tag for relative and absolute quantitation (iTRAQ) and tandem mass tags (TMTs) due to their synthetic simplicity, labeling efficiency and improved fragmentation efficiency.
    Type: Application
    Filed: September 14, 2012
    Publication date: March 28, 2013
    Inventors: Lingjun Li, Feng Xiang, Tyler Greer, Dustin Frost, Zhidan Liang