Abstract: A nanodelivery system based on globulin-1 S allele, a preparation method therefor and use thereof are provided. The preparation method includes the following steps of: dissolving globulin-1 S allele in a NaCl solution, and performing magnetical stirring until the globulin-1 S allele is completely dissolved to obtain a mixed solution; self-assembly: centrifuging the mixed solution, taking the supernatant to be dispersed into a dispersion solution for self-assembly to obtain a globulin-1 S allele dispersion; and adding hydrophobic plant polyphenol to the globulin-1 S allele dispersion, performing well mixing and centrifuging, and removing the supernatant to obtain the nanodelivery system based on the globulin-1 S allele.

Abstract: A method for simultaneous detection of multiple allergens in food includes: diluting a plurality of target allergen proteins in a concentration gradient, adding them to an ELISA plate, and incubating overnight; washing the ELISA plate with a PBST washing solution to remove unbound proteins; adding 5% BSA blocking reagent to the ELISA plate, incubating with shaking, and washing away the blocking reagent; premixing aptamers of the plurality of target allergen proteins into a premixed solution; adding the premixed solution to the ELISA plate from which the blocking reagent is washed away, incubating with shaking, and washing away the premixed solution; adding ultrapure water to the ELISA plate, incubating at 95° C. to denature the allergen proteins, and recovering a supernatant containing the aptamers; using the supernatant as a DNA template, performing Taqman qPCR amplification and fluorescence signal detection to obtain a reaction ct value; and plotting a standard curve.

Abstract: The present invention belongs to the field of drug delivery and food and pharmaceutical technology, and specifically relates to resistant starch for intestinal-targeted sustained-release of polyphenols, and a preparation method and application thereof, comprising the following steps: preparing quinoa polyphenol extract; annealing and recrystallizing heat-dispersed high-amylose maize starch in an ethanol solution to obtain single-helix resistant maize starch; self-assembling single-helix resistant maize starch and quinoa polyphenol extract in an alcohol-water system to obtain intestinal-targeted sustained-release of polyphenol/resistant starch.

Abstract: An active human milk oligosaccharide (HMO) preparation with an allergy-alleviating function is provided. The active HMO preparation includes one or more of lacto-N-fucopentaose I (LNFP-I) and 2?-fucosyllactose (2?-FL). A part of these two HMOs ingested by a human body can enter a circulatory system to regulate the immune cell populations and the cytokine secretion, so as to directly affect an immune system of the human body and well alleviate the allergies in some infants and young children. In addition, for infants and young children who have a human milk without 2?-FL and LNFP-I, the active HMO preparation can supplement the nutrients of the human milk.

Abstract: A method and a kit for detecting a content of wheat gluten in food are provided. The kit includes a Raman enhanced substrate solution and a quartz sheet, where the Raman enhanced substrate solution includes a gold nanorod dimer and a Raman signal molecule, where the gold nanorod dimer is formed by self-assembling a gold nanorod functionalized by aptamer, and a gold nanorod functionalized by an aptamer complementary chain. The kit has simple and convenient operation and a convenient instrument, and can quickly, stably and sensitively detect the content of the wheat gluten in the food.

Abstract: A nanodelivery system based on globulin-1 S allele, a preparation method therefor and use thereof are provided. The preparation method includes the following steps of: dissolving globulin-1 S allele in a NaCl solution, and performing magnetical stirring until the globulin-1 S allele is completely dissolved to obtain a mixed solution; self-assembly: centrifuging the mixed solution, taking the supernatant to be dispersed into a dispersion solution for self-assembly to obtain a globulin-1 S allele dispersion; and adding hydrophobic plant polyphenol to the globulin-1 S allele dispersion, performing well mixing and centrifuging, and removing the supernatant to obtain the nanodelivery system based on the globulin-1 S allele.

Abstract: The present invention discloses Lactobacillus sakei for alleviating allergy and a preparation method thereof, and particularly Lactobacillus sakei for alleviating wheat-dependent, exercise-induced anaphylaxis (WDEIA). The Lactobacillus sakei is classified and named as Lactobacillus sakei. The Lactobacillus sakei has been preserved in China Center for Type Culture Collection since Apr. 12, 2003, with a preservation number of CCTCC DB 20082413. The Lactobacillus sakei can regulate the immune function of a human body to prevent/treat WDEIA by reducing the contents of specific IgE and histamine in serum of WDEIA patients.

Abstract: A method for establishing an epicutaneously sensitized food allergy animal model and belongs to the technical field of medical evaluation and detection. The method includes an allergen sensitization stage and an allergen challenge stage, wherein an MC903 ethanol solution containing OVA is smeared on the skin of experimental animals in the allergen sensitization stage, and the OVA dissolved in a buffer solution is used for an oral challenge in the allergen challenge stage.

Abstract: The present invention provides a PEG-ACS/M-siRNA nanocomposite application, and method for reducing the histamine content during fishmeal storage thereof. A small interfering ribonucleic acid (siRNA) is designed and prepared according to a histidine decarboxylase gene of Morganella morganii (Morganella morganii subsp. morganii KT), and the histidine decarboxylase gene has a sequence of SEQ ID No: 1. A PEG-ACS/M-siRNA nanocomposite is prepared by using a PEGylated arginine-modified chitosan as a carrier. A PEG-ACS/M-siRNA nanocomposite is added to fishmeal in a certain ratio. The method for reducing the histamine content during fishmeal storage has a significant inhibitory effect on the histamine content during fishmeal storage, and can reduce the histamine content in fishmeal by 49%-53%, which has great significance for the control of biogenic amines in fishmeal in the feed industry.

Abstract: The present invention provides a PEG-ACS/M-siRNA nanocomposite application, and method for reducing the histamine content during fishmeal storage thereof. A small interfering ribonucleic acid (siRNA) is designed and prepared according to a histidine decarboxylase gene of Morganella morganii (Morganella morganii subsp. morganii KT), and the histidine decarboxylase gene has a sequence of SEQ ID No: 1. A PEG-ACS/M-siRNA nanocomposite is prepared by using a PEGylated arginine-modified chitosan as a carrier. A PEG-ACS/M-siRNA nanocomposite is added to fishmeal in a certain ratio. The method for reducing the histamine content during fishmeal storage has a significant inhibitory effect on the histamine content during fishmeal storage, and can reduce the histamine content in fishmeal by 49%-53%, which has great significance for the control of biogenic amines in fishmeal in the feed industry.

Abstract: The invention relates to an integral chisel, and more particularly to a combined integral chisel having high efficiency, including a main body. A head portion of the main body is a chisel head portion, an end portion of the main body is a grab handle portion. The chisel head, the main body and the grab handle portion are integrally distributed. The combined integral chisel having high efficiency has advantages of compact structure, excellent performance, and improved operational safety performance.