Abstract: The present disclosure provides a method for increasing a fermentation yield of PF1022A, the method comprises taking a Rosellinia sp. capable of producing PF1022A as a production strain and performing aerobic fermentation in a fermentation medium, the fermentation medium comprises an assimilable carbon source, an assimilable nitrogen source and an inorganic salt, wherein the assimilable carbon source contains solid malt extract. The technical solution provided by the present disclosure is simple and easy to implement, improves the production efficiency of PF1022A, greatly increases the output of PF1022A, reduces the production costs, and facilitates large-scale industrial production of PF1022A.

Abstract: The invention discloses a method for the separation and purification of epothilones, especially discloses a method for the separation and purification of epothilones B and A using normal phase silica gel chromatography, which comprises loading the sample after dissolving the sample containing epothilones B and A with C1-C7 alkyl halide compounds or mixing the sample with silica gel, then gradient eluting silica gel column by an elution solvent of normal phase silica gel column, and finally obtaining products.

Abstract: The invention discloses a method for the separation and purification of epothilones, especially discloses a method for the separation and purification of epothilones B and A using normal phase silica gel chromatography, which comprises loading the sample after dissolving the sample containing epothilones B and A with C1-C7 alkyl halide compounds or mixing the sample with silica gel, then gradient eluting silica gel column by an elution solvent of normal phase silica gel column, and finally obtaining products.