Abstract: A method for sterilizing and preserving fresh mulberry fruits is provided. The method includes the following steps: 1) laying the fresh mulberry fruits in a single layer in a hermetic container, and 2) introducing atmospheric plasma into the hermetic container for sterilization to obtain sterilized fresh mulberry fruits. The method provided in the present disclosure can effectively inactivate bacteria, yeast, and fungi on surfaces of the mulberry fruits, such as Botrytis cinerea, Salmonella, Escherichia coli, Staphylococcus aureus, and Bacillus cereus. The method provided in the present disclosure has no significant impact on qualities of the mulberry fruit such as pH, total soluble solids (TSS), hardness, and color, and can significantly reduce rotting incidence and mildew incidence of the mulberry fruits. The rotting incidence can be reduced by up to 30.00% and the mildew incidence can be reduced by up to 25.14%.