Patents by Inventor Lingyu HE

Lingyu HE has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20180291371
    Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.
    Type: Application
    Filed: November 26, 2014
    Publication date: October 11, 2018
    Applicant: BGI Shenzhen Co., Ltd.
    Inventors: Chunyu Geng, Ruoying Chen, Yuan Jiang, Xia Zhao, Rongrong Guo, Lingyu He, Yaqiao Li, Wenwei Zhang, Hui Jiang, Radoje Drmanac
  • Patent number: 10023906
    Abstract: Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagent kit thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5? end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.
    Type: Grant
    Filed: October 14, 2014
    Date of Patent: July 17, 2018
    Assignee: MGI Tech Co., Ltd.
    Inventors: Chunyu Geng, Rongrong Guo, Ruoying Chen, Lingyu He, Wenwei Zhang, Hui Jiang
  • Publication number: 20180044667
    Abstract: Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagents thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5? end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.
    Type: Application
    Filed: October 14, 2014
    Publication date: February 15, 2018
    Inventors: Chunyu Geng, Rongrong Guo, Ruoying Chen, Lingyu He, Wenwei Zhang, Hui Jiang
  • Patent number: 9890375
    Abstract: Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5?-end nucleotide of the first strand has a phosphate group, and the 3?-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5?-end nucleotide of the second strand does not have a phosphate group, and the 3?-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.
    Type: Grant
    Filed: September 12, 2014
    Date of Patent: February 13, 2018
    Assignee: BGI SHENZHEN CO., LIMITED
    Inventors: Chunyu Geng, Dennis G. Ballinger, Yanyan Zhang, Shujin Fu, Lingyu He, Wenwei Zhang, Hui Jiang
  • Publication number: 20170275609
    Abstract: Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5?-end nucleotide of the first strand has a phosphate group, and the 3?-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5?-end nucleotide of the second strand does not have a phosphate group, and the 3?-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.
    Type: Application
    Filed: September 12, 2014
    Publication date: September 28, 2017
    Inventors: Chunyu Geng, Dennis G. Ballinger, Yanyan Zhang, Shujin Fu, Lingyu He, Wenwei Zhang, Hui Jiang
  • Publication number: 20170233728
    Abstract: Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5? end of the long strand has a phosphoric acid modification, and the 3? end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3? end thereof can be in a complementary pairing with the 5? end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.
    Type: Application
    Filed: October 14, 2014
    Publication date: August 17, 2017
    Inventors: Yuan JIANG, Chunyu GENG, Xia ZHAO, Shujin FU, Lingyu HE, Yaqiao LI, Xiaoshan SU, Fanzi WU, Wenwei ZHANG, Hui JIANG, Andrei ALEXEEV, Radoje DRMANAC