Patents by Inventor Linka Yu

Linka Yu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • ?-Transaminase mutant obtained by DNA synthetic shuffling combined mutation and use
    Patent number: 12152255
    Abstract: The present invention discloses a ?-transaminase mutant obtained through DNA synthetic shuffling combined mutation. The ?-transaminase mutant is obtained through point mutation of a wild type ?-transaminase from Aspergillus terrus. The amino acid sequence of the wild type ?-transaminase is shown in SEQ ID NO: 1. The mutation site of the ?-transaminase mutant is any one of: (1) F115L-H210N-M150C-M280C; (2) F115L-H210N; (3) F115L-H210N-E253A-I295V; (4) I77L-F115L-E133A-H210N-N245D; (5) I77L-Q97E-F115L-L118T-E253A-G292D; (6) I77L-E133A-N245D-G292D; and (7) H210N-N245D-E253A-G292D. According to the present invention, forward mutations obtained in the previous stage are randomly combined through a DNA synthetic shuffling combined mutation method.
    Type: Grant
    Filed: December 6, 2021
    Date of Patent: November 26, 2024
    Assignees: ZHEJIANG UNIVERSITY OF SCIENCE & TECHNOLOGY, ENZYMASTER (NINGBO) BIO-ENGINEERING CO., LTD.
    Inventors: Jun Huang, Chunyan Liu, Lehe Mei, Haibin Chen, Changjiang Lv, Sheng Hu, Hongpeng Wang, Weirui Zhao, Fangfang Fan, Ye Li, Linka Yu, Yifeng Zhou
  • OMEGA-TRANSAMINASE MUTANT OBTAINED BY DNA SYNTHETIC SHUFFLING COMBINED MUTATION AND USE
    Publication number: 20220177933
    Abstract: The present invention discloses a ?-transaminase mutant obtained through DNA synthetic shuffling combined mutation. The ?-transaminase mutant is obtained through point mutation of a wild type ?-transaminase from Aspergillus terrus. The amino acid sequence of the wild type ?-transaminase is shown in SEQ ID NO: 1. The mutation site of the ?-transaminase mutant is any one of: (1) F115L-H210N-M150C-M280C; (2) F115L-H210N; (3) F115L-H210N-E253A-I295V; (4) I77L-F115L-E133A-H210N-N245D; (5) I77L-Q97E-F115L-L118T-E253A-G292D; (6) I77L-E133A-N245D-G292D; and (7) H210N-N245D-E253A-G292D. According to the present invention, forward mutations obtained in the previous stage are randomly combined through a DNA synthetic shuffling combined mutation method.
    Type: Application
    Filed: December 6, 2021
    Publication date: June 9, 2022
    Inventors: JUN HUANG, Chunyan Liu, Lehe Mei, Haibin Chen, Changjiang LV, Sheng Hu, Hongpeng Wang, Weirui Zhao, Fangfang Fan, Ye Li, Linka Yu, Yifeng Zhou