Abstract: Provided are an AAV capsid protein mutant and an encoding nucleic acid, and a corresponding vector and a host cell thereof. Also provided are an adeno-associated virus vector containing the AAV capsid protein mutant, a recombinant adeno-associated virus particle constructed therefrom and carrying a gene expression cassette, a preparation method therefor, and the use thereof in treating diseases.

Abstract: The disclosure provides a novel design of guide RNA and uses thereof. In one aspect, the guide RNA comprises a spacer sequence flanked by a direct repeat sequence at both the 5? end and the 3? end of the spacer sequence.

Abstract: The invention provides novel engineered CRISPR/Cas effector enzymes, such as Cas13 (e.g., Cas13e) that substantially maintain guide-sequence-specific endonuclease activity and substantially lack guide-sequence-independent collateral endonuclease activity compared to the corresponding wild-type Cas. Also provided are polynucleotides encoding the same, vectors or host cells comprising the polynucleotides or engineered Cas, and method of use, such as in RNA-based target gene transcript knock down.

Abstract: The invention described herein provides a recombinant DNA viral particle comprising a protein shell encapsulating an RNA vector genome, as well as related compositions and uses thereof.

Abstract: Provided is an AAV mutant that efficiently infects supporting cells. Specifically, provided is a gene expression vector used for treating hearing disorders, and the described expression vector is an AAV mutant. In the AAV mutant, an expression cassette of a therapeutic gene used for treating hearing disorders is inserted or carried, and additionally, the AAV mutant contains a gene sequence that encodes a viral capsid protein mutant.

Abstract: The invention discloses an absorbent and permeable ceramic and a preparation method and application thereof. The absorbent and permeable ceramic is prepared by the steps of: adding water to the following raw components in parts by weight: 1-5 parts of bauxite, 1-3 parts of quartz, 1-3 parts of black soil and 1 part of crude pottery and then kneading evenly; flapping and shaping the evenly kneaded mixed mud; drying the shaped mixed mud under room temperature, biscuiting, supplementing water to wipe out the dust on the surfaces of the mixed mud, and sintering the mixed mud in a redox atmosphere in a kiln to obtain the absorbent and permeable ceramic, wherein the sintering temperature is in the range of 1280-1350° C. and the sintering duration is in the range of 10-12 h.