Abstract: A modified vector of adenovirus-associated virus serotype 8 (AAV-8) for gene targeting and expression is provided, wherein the modified vector includes serotype coat amino acid sequence, insertion site and insertion amino acid sequence. A 10-amino acid sequence set forth in SEQ ID NO. 3 is inserted between amino acids at positions 590 and 591 that are set forth in SEQ ID NO. 2 of the AAV-8 serotype coat protein: LARGDSTKSA, wherein amino acids at positions 1, 2 and 10 are protective amino acids, and amino acids at positions 3 to 9 are screened amino acid sequences. In addition, the present invention also discloses construction method and application of the modified vector. The modified vector of the present invention has stronger fluorescence, can be observed obviously and intuitively. Exogenous gene can be efficiently expressed in the targeted tissues in vivo.

Abstract: A gear shifting device for electric cars comprises a shell, a first switch, a second switch and an operating part, wherein a first control button of the first switch and a second control button of the second switch are oppositely arranged, the operating part has an upper portion rotatably connected to the shell and a lower portion provided with an operating rod located between the first control button and the second control button, the first control button is located on a left side of a front-back moving direction of the operating rod, and the second control button is located on a right side of the front-back moving direction of the operating rod. The operating rod is located between the two switches and can move to switch the switches to different positions, and thus, the gear shifting device is ingenious and safe.