Abstract: A hybridoma cell line of secreting clarithromycin monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14696 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with clarithromycin complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three subclones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to clarithromycin (value of IC50 is 0.3 ng/ml), being suitable for detection of clarithromycin in food.

Abstract: The disclosure relates to a system and method for targeted advertising based on the back of cash register paper. The system includes a cloud advertisement server, at least one advertisement releasing terminal, and a plurality of double-sided printers; the plurality of double-sided printers are pre-divided into a plurality of specified classifications; the advertisement releasing terminal is used for receiving advertisement contents input by a user, generating advertisement information, and sending same to the cloud advertisement server; the cloud advertisement server is used for generating corresponding advertisement pushing information and sending same to the double-sided printers of the specified classifications; the double-sided printers are used for receiving the advertisement pushing information and printing corresponding advertisement information on a back of cash register paper.

Abstract: Provided are an intelligent advertisement system and method based on a duplex printer, the method including steps of: receiving, through an advertisement releasing terminal, advertisement content input by a user, generating advertisement information and then sending the advertisement information to a cloud advertisement server; receiving, through the cloud advertisement server, the advertisement information sent by the advertisement releasing terminal, and then pushing the advertisement information to each duplex printer; receiving, through each duplex printer, the advertisement information and printing the advertisement information on a back of cash register paper while printing cash register information; and playing, through each printer control terminal, a corresponding advertisement prompt message based on the advertisement information printed by the duplex printer.

Abstract: An intelligent advertisement publishing system and method based on a double-sided printer distribution map. The method includes: generating a distribution map based on geographical location information of double-sided printers; displaying the distribution map of the double-sided printers, and after obtaining advertisement content input by a user and an advertisement publishing scope selected by the user on the distribution map, generating advertisement information; parsing to obtain the corresponding advertisement publishing scope and advertisement content, generating corresponding advertisement push information based on the advertisement content, and sending the advertisement push information to double-sided printers in the advertisement publishing scope; and enabling the double-sided printers to print the corresponding advertisement information at the back of cash register paper when printing cash registration information.

Abstract: Disclosed is a big data advertisement system and method for advertising through a back of cash register paper. The system comprises a cloud advertisement server, at least one advertisement releasing terminal, and a plurality of double-sided printers, wherein the cloud advertisement server is configured for receiving advertisement information sent by the advertisement releasing terminal, generating corresponding advertisement pushing information, and then sending the corresponding advertisement pushing information to the double-sided printers, and also configured for outputting an intelligent maintenance advice or advertisement statistical information, the double-sided printers are configured for receiving the advertisement pushing information and printing the corresponding advertisement information on the back of the cash register paper when printing cashier information, and sending real-time working parameters and real-time printing data to the cloud advertisement server.

Abstract: The present disclosure discloses a lincosamides universal monoclonal antibody hybridoma cell strain and application thereof, and belongs to the technical field of food safety immunological detection. According to the present disclosure, a clindamycin chlorine-substituted derivative is used as a hapten, the hapten is coupled with bovine serum albumin (BSA) by an activated ester method to obtain an immunizing antigen, and after being uniformly mixed with a Freund's adjuvant, the immunizing antigen is subcutaneously injected to immunize BALB/c mice; clindamycin is coupled with ovalbumin (OVA) by a carbonyl diimidazole (CDI) method to be used as a coating antigen used for detecting mouse serums and a cell supernatant. The spleen cells of the immunized mice are fused with mouse myeloma cells by a PEG method, and screened by indirect ELISA and indirect competitive ELISA and subcloned three times to obtain a population-selective hybridoma cell strain.

Abstract: A hybridoma cell line of secreting meloxicam monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14700 belongs to the field of food safety immunological detection. The. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with meroxicam complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to meloxicam (value of IC50 is 0.1 ng/ml), being suitable for detection of meroxicam in food.

Abstract: A hybridoma cell strain secreting a nifursolol residue marker monoclonal antibody with CGMCC No. 14698 is prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursolol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 ?g/L) to the nifursolol residue marker and can be used for residue detection of the nifursolol residual marker in food.

Abstract: A hybridoma cell line of secreting meloxicam monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14700 belongs to the field of food safety immunological detection. The. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with meroxicam complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to meloxicam (value of IC50 is 0.1 ng/ml), being suitable for detection of meroxicam in food.

Abstract: The present invention provides an actually-measured marine environment data assimilation method based on sequence recursive filtering three-dimensional variation. The method includes: preprocessing actually-measured marine environment data; calculating a target function value; calculating a gradient value of a target function; calculating a minimum value of the target function; extracting space multi-scale information from the actually-measured data; and updating background field data to form a final data assimilation analysis field. The present invention improves the traditional recursive filtering three-dimensional variation method, and sequentially assimilates information with different scales, thereby effectively overcoming the problem that multi-scale information cannot be effectively extracted by a traditional three-dimensional variation method.

Abstract: A hybridoma cell line of secreting clarithromycin monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14696 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with clarithromycin complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three subclones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to clarithromycin (value of IC50 is 0.3 ng/ml), being suitable for detection of clarithromycin in food.

Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.

Abstract: Embodiments of this application disclose an interaction information obtaining method performed at a target user terminal. After detecting, on a virtual map preset in an interaction application, a trigger operation performed on a target POI identifier, the target user terminal obtains first location information of the target user terminal by using the virtual map and second location information corresponding to the target POI identifier on the virtual map. The target user terminal detects a distance difference between the first location information and the second location information. When the distance difference satisfies a preset distance range, the target user terminal sends, to an interaction server, an interaction information request carrying the target POI identifier and receives interaction information corresponding to the target POI identifier from the interaction server and then displays the interaction information on the virtual map.

Abstract: The present disclosure provides a method and apparatus for transmitting data, and an electronic device thereof. The method includes identifying a first data object in a first object set in response to transmitting the first object set from a first storage area to a second storage area; and transmitting the first data object to the second storage area to indicate a start of transmission of the first object set from the first storage area to the second storage area.

Abstract: A hybridoma cell line of secreting clarithromycin monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14696 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with clarithromycin complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three subclones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to clarithromycin (value of IC50 is 0.3 ng/ml), being suitable for detection of clarithromycin in food.

Abstract: The present invention provides an actually-measured marine environment data assimilation method based on sequence recursive filtering three-dimensional variation. The method includes: preprocessing actually-measured marine environment data; calculating a target function value; calculating a gradient value of a target function; calculating a minimum value of the target function; extracting space multi-scale information from the actually-measured data; and updating background field data to form a final data assimilation analysis field. The present invention improves the traditional recursive filtering three-dimensional variation method, and sequentially assimilates information with different scales, thereby effectively overcoming the problem that multi-scale information cannot be effectively extracted by a traditional three-dimensional variation method.

Abstract: One embodiment provides a method, including: obtaining partial information content from a first file, wherein the partial information content is recorded in the first file and comprises an indication of a selection of at least a portion of the first file; generating a second file different from the first file based on the partial information content; and sharing the second file with at least one electronic device. Other aspects are described and claimed.