Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.

Abstract: A method for evaluating an aging state of a silicone rubber composite insulator includes: step 1: collecting samples from the silicone rubber composite insulator, and subjecting samples in a surface layer and a center layer successively to hydrophobicity, flexing resistance, hardness, color, and absorbance tests; step 2: determining relevant data of the samples in a natural evolution process according to the tests in step 1; step 3: subjecting the samples to thermo-oxidative aging; and after the thermo-oxidative aging is completed, subjecting the samples to a hardness test of artificial accelerated evolution, and calculating position coordinates (F1, F2) of the samples in an aging space; and step 4: classifying the samples in advance and solving an aging state centroid of each classified population, calculating a minimum aging distance based on the position coordinates in step 3, and evaluating the aging state of the silicone rubber composite insulator.

Abstract: A method for evaluating an aging state of a silicone rubber composite insulator includes: step 1: collecting samples from the silicone rubber composite insulator, and subjecting samples in a surface layer and a center layer successively to hydrophobicity, flexing resistance, hardness, color, and absorbance tests; step 2: determining relevant data of the samples in a natural evolution process according to the tests in step 1; step 3: subjecting the samples to thermo-oxidative aging; and after the thermo-oxidative aging is completed, subjecting the samples to a hardness test of artificial accelerated evolution, and calculating position coordinates (F1, F2) of the samples in an aging space; and step 4: classifying the samples in advance and solving an aging state centroid of each classified population, calculating a minimum aging distance based on the position coordinates in step 3, and evaluating the aging state of the silicone rubber composite insulator.

Abstract: The present invention belongs to the field of pharmaceutical chemistry, and relates to a substituted pyrazine compound, a pharmaceutical composition comprising same, and the use thereof. In particular, the present invention relates to a compound with the structure of formula (I), which exhibits a good SHP2 inhibiting activity, and can act as an efficient SHP2 inhibitor for preventing and/or treating SHP2-related diseases.