Abstract: A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng/ml), being suitable for detection of cyproheptadine in food.

Abstract: A display substrate and a preparation method thereof, and a display device. The display substrate comprises a display area (100), a bonding area (200), and a side bezel region (310) at least including a first bezel region (310-1) and a second bezel region (310-2), the first bezel region (310-1) is located on a side of the second bezel region (310-2) close to the bonding area (200); the display substrate comprises a base substrate (101) and a drive circuit layer (102), the base substrate (101) at least comprises a first connection line (70), the drive circuit layer (102) at least comprises a data signal line (60), a second connection line (80) connected to the first connection line through a first lap via (DV1), the data signal line is connected to the second connection line through a second lap via (DV2), the first lap via is provided in the first bezel region.

Abstract: The present disclosure discloses a microfluidic chip, a system and a method for automatic separation and detection of circulating tumor cells. The microfluidic chip includes a density gradient centrifugation assembly, a cell capture assembly, and a reagent storage assembly. The density gradient centrifugation assembly is configured to carry out density gradient centrifugation on a whole blood sample to achieve separation and obtain a plasma layer, a monocyte layer, a Ficoll solution layer, and an erythrocyte and granulocyte layer. The cell capture assembly includes a capture channel, a capture inlet, and a capture outlet. The capture inlet and the capture outlet are in communication with the capture channel. An inner wall of the capture channel is provided with capture holes and negative-pressure capture chambers. Each capture hole is in communication with one negative-pressure capture chamber correspondingly.

Abstract: The disclosure relates to the technical field of virus detection, in particular, to an IgA antibody and a kit capable of specifically recognizing RBD protein. The antibody can be used as a calibrator of IgA antibody against the RBD protein of SARS-CoV-2.