Abstract: The disclosure provides devices, methods and systems for continuous dielectrophoresis cell sorting to isolate different populations of cells, and applications thereof.

Abstract: A method for enriching a heterogeneous population of cells includes loading one or more sample chambers containing DEP electrodes therein with a solution containing the heterogeneous population of cells, wherein the heterogeneous population of cells comprises a first subpopulation of cells having a first crossover frequency and a second subpopulation of cells having a second, higher crossover frequency. An AC electrical field is applied to the DEP electrodes, wherein the AC electrical field has an applied frequency that is between the crossover frequency of the first subpopulation of cells and the second subpopulation of cells, wherein the first subpopulation of cells are substantially killed by the applied electrical field and the second subpopulation of cells are substantially not killed by the applied electrical field.

Abstract: A method for enriching a heterogeneous population of cells includes loading one or more sample chambers containing DEP electrodes therein with a solution containing the heterogeneous population of cells, wherein the heterogeneous population of cells comprises a first subpopulation of cells having a first crossover frequency and a second subpopulation of cells having a second, higher crossover frequency. An AC electrical field is applied to the DEP electrodes, wherein the AC electrical field has an applied frequency that is between the crossover frequency of the first subpopulation of cells and the second subpopulation of cells, wherein the first subpopulation of cells are substantially killed by the applied electrical field and the second subpopulation of cells are substantially not killed by the applied electrical field.

Abstract: A microfluidic separation device includes a microchannel formed in a substrate and being defined at least by a bottom surface, a first side wall, and second side wall. Fluid containing particles or cells is flowed through the microchannel from an upstream end to a downstream end. The downstream end terminates in a plurality of branch channels. A plurality of vertically-oriented electrodes are disposed on the first wall and on the second wall opposite to the first wall. A voltage source is connected to the plurality of opposing electrodes to drive the electrodes. The opposing, vertically-oriented electrodes may be used to focus a heterogeneous population of particles or cells for subsequent downstream separation via additional electrodes placed on one of the side walls. Alternatively, the opposing, vertically-oriented electrodes may be used to spatially separate a heterogeneous population of particles or cells for later collection in one or more of the branch channels.

Abstract: A microfluidic separation device includes a microchannel formed in a substrate and being defined at least by a bottom surface, a first side wall, and second side wall. Fluid containing particles or cells is flowed through the microchannel from an upstream end to a downstream end. The downstream end terminates in a plurality of branch channels. A plurality of vertically-oriented electrodes are disposed on the first wall and on the second wall opposite to the first wall. A voltage source is connected to the plurality of opposing electrodes to drive the electrodes. The opposing, vertically-oriented electrodes may be used to focus a heterogeneous population of particles or cells for subsequent downstream separation via additional electrodes placed on one of the side walls. Alternatively, the opposing, vertically-oriented electrodes may be used to spatially separate a heterogeneous population of particles or cells for later collection in one or more of the branch channels.

Abstract: A process of using a fish plasma component for tissue engineering includes obtaining a fish that is a progeny of domesticated broodstock that are reared under consistent and reproducible conditions. Blood is obtained from the fish. Plasma is separated from the blood. One or more specific components of the plasma are extracted. Tissue is engineered using the one or more extracted plasma components, and none of any remainder of the plasma.

Abstract: A tissue culture substrate for culturing mammalian stem cell tissue includes one or more specific fish plasma fractions, isolated from any plasma remainder, in a nutrient medium. The plasma fractions are extracted from plasma obtained from the blood of one or more fish that are progeny of domesticated broodstock reared under consistent and reproducible conditions.

Abstract: A process of using a fish plasma component as a nutrient medium component for tissue culture includes obtaining blood from a fish that is a progeny of domesticated broodstock that are reared under consistent and reproducible conditions, separating plasma from the blood, and extracting one or more specific components of the plasma. The tissue is cultured using the extracted plasma components, and none of any remainder of the plasma, in a nutrient medium. The tissue cultured using the extracted plasma component is other than fish tissue, such as mammalian tissue or insect tissue.

Abstract: A process of using a fish plasma component as a nutrient medium component for tissue culture includes obtaining blood from a fish, separating plasma from the blood, and extracting one or more specific components of the plasma. The tissue is cultured using the extracted plasma components, and none of any remainder of the plasma, in a nutrient medium. The tissue cultured using the extracted plasma component is other than fish tissue, such as mammalian tissue or insect tissue.

Abstract: A process of using a fish plasma component as a nutrient medium component for tissue culture includes obtaining blood from a fish, separating plasma from the blood, and extracting one or more specific components of the plasma. The tissue is cultured using the extracted plasma components, and none of any remainder of the plasma, in a nutrient medium. The tissue cultured using the extracted plasma component is other than fish tissue, such as mammalian tissue or insect tissue.