Abstract: A real time assay monitoring system and method can be used to monitor reagent volume in assays for fluid replenishment control, monitor assays in real-time to obtain quality control information, monitor assays in real-time during development to detect saturation levels that can be used to shorten assay time, and provide assay results before the assay is complete, enabling reflex testing to begin automatically. The monitoring system can include a real time imaging system with a camera and lights to capture images of the assay. The captured images can then be used to monitor and control the quality of the staining process in an assay, provide early assay results, and/or to measure the on-site reagent volume within the assay.

Abstract: A real time assay monitoring system and method can be used to monitor reagent volume in assays for fluid replenishment control, monitor assays in real-time to obtain quality control information, monitor assays in real-time during development to detect saturation levels that can be used to shorten assay time, and provide assay results before the assay is complete, enabling reflex testing to begin automatically. The monitoring system can include a real time imaging system with a camera and lights to capture images of the assay. The captured images can then be used to monitor and control the quality of the staining process in an assay, provide early assay results, and/or to measure the on-site reagent volume within the assay.

Abstract: A real time assay monitoring system and method can be used to monitor reagent volume in assays for fluid replenishment control, monitor assays in real-time to obtain quality control information, monitor assays in real-time during development to detect saturation levels that can be used to shorten assay time, and provide assay results before the assay is complete, enabling reflex testing to begin automatically. The monitoring system can include a real time imaging system with a camera and lights to capture images of the assay. The captured images can then be used to monitor and control the quality of the staining process in an assay, provide early assay results, and/or to measure the on-site reagent volume within the assay.

Abstract: A real time assay monitoring system and method can be used to monitor reagent volume in assays for fluid replenishment control, monitor assays in real-time to obtain quality control information, monitor assays in real-time during development to detect saturation levels that can be used to shorten assay time, and provide assay results before the assay is complete, enabling reflex testing to begin automatically. The monitoring system can include a real time imaging system with a camera and lights to capture images of the assay. The captured images can then be used to monitor and control the quality of the staining process in an assay, provide early assay results, and/or to measure the on-site reagent volume within the assay.

Abstract: A real time assay monitoring system and method can be used to monitor reagent volume in assays for fluid replenishment control, monitor assays in real-time to obtain quality control information, monitor assays in real-time during development to detect saturation levels that can be used to shorten assay time, and provide assay results before the assay is complete, enabling reflex testing to begin automatically. The monitoring system can include a real time imaging system with a camera and lights to capture images of the assay. The captured images can then be used to monitor and control the quality of the staining process in an assay, provide early assay results, and/or to measure the on-site reagent volume within the assay.

Abstract: A real time assay monitoring system and method can be used to monitor reagent volume in assays for fluid replenishment control, monitor assays in real-time to obtain quality control information, monitor assays in real-time during development to detect saturation levels that can be used to shorten assay time, and provide assay results before the assay is complete, enabling reflex testing to begin automatically. The monitoring system can include a real time imaging system with a camera and lights to capture images of the assay. The captured images can then be used to monitor and control the quality of the staining process in an assay, provide early assay results, and/or to measure the on-site reagent volume within the assay.

Abstract: A real-time imaging method and system for monitoring an assay process performed on a biological sample is described. In some embodiments, the method and system can be used to measure and control a reagent volume confined to a space between a cover and a substrate, which is particularly useful for controlling for evaporation in a thin-film staining environment. In particular embodiments, the disclosed reagent volume sensing and replenishment method and system are resistant to system noises generated during operation, for example, system noise due to vibration, field of view blockage by dispenser systems, differences in reagent colors, and changing tissue colors.

Abstract: A method for estimating and tracking refractive error progression of an individual includes estimating a percentile of Spherical Equivalent Refraction (SPHEQ) as a function of at least the individual's age by comparison to a reference population; estimating an expected SPHEQ trajectory over a future predetermined period of time; comparing the expected SPHEQ trajectory with the reference population; and comparing the expected SPHEQ trajectory with an expected SPHEQ trajectory using an ametropia control treatment, thereby showing a possible treatment benefit over the predetermined period of time.

Abstract: A real time assay monitoring system and method can be used to monitor reagent volume in assays for fluid replenishment control, monitor assays in real-time to obtain quality control information, monitor assays in real-time during development to detect saturation levels that can be used to shorten assay time, and provide assay results before the assay is complete, enabling reflex testing to begin automatically. The monitoring system can include a real time imaging system with a camera and lights to capture images of the assay. The captured images can then be used to monitor and control the quality of the staining process in an assay, provide early assay results, and/or to measure the on-site reagent volume within the assay.

Abstract: The present invention relates to a system and method for monitoring changes in reagent concentration, and in particular, to a system and method for monitoring for changes in reagent concentrations within small volumes of liquids in contact with a biological sample disposed on a substrate such as a microscope slide. The disclosed system and method utilize changes in electromagnetic properties associated with an interface between the substrate and a liquid applied to the biological sample in order to provide information regarding changes in reagent concentration within the liquid.

Abstract: A real-time imaging method and system for monitoring an assay process performed on a biological sample is described. In some embodiments, the method and system can be used to measure and control a reagent volume confined to a space between a cover and a substrate, which is particularly useful for controlling for evaporation in a thin-film staining environment. In particular embodiments, the disclosed reagent volume sensing and replenishment method and system are resistant to system noises generated during operation, for example, system noise due to vibration, field of view blockage by dispenser systems, differences in reagent colors, and changing tissue colors.

Abstract: A method for estimating and tracking refractive error progression of an individual includes estimating a percentile of Spherical Equivalent Refraction (SPHEQ) as a function of at least the individual's age by comparison to a reference population; estimating an expected SPHEQ trajectory over a future predetermined period of time; comparing the expected SPHEQ trajectory with the reference population; and comparing the expected SPHEQ trajectory with an expected SPHEQ trajectory using an ametropia control treatment, thereby showing a possible treatment benefit over the predetermined period of time.

Abstract: A method for estimating and tracking refractive error progression of an individual includes estimating a percentile of Spherical Equivalent Refraction (SPHEQ) as a function of at least the individual's age by comparison to a reference population; estimating an expected SPHEQ trajectory over a future predetermined period of time; comparing the expected SPHEQ trajectory with the reference population; and comparing the expected SPHEQ trajectory with an expected SPHEQ trajectory using an ametropia control treatment, thereby showing a possible treatment benefit over the predetermined period of time.

Abstract: A method for estimating and tracking refractive error progression of an individual includes estimating a percentile of Spherical Equivalent Refraction (SPHEQ) as a function of at least the individual's age by comparison to a reference population; estimating an expected SPHEQ trajectory over a future predetermined period of time; comparing the expected SPHEQ trajectory with the reference population; and comparing the expected SPHEQ trajectory with an expected SPHEQ trajectory using an ametropia control treatment, thereby showing a possible treatment benefit over the predetermined period of time.