Abstract: The present invention relates to a nanosensor for detecting SARS-CoV-2 specific antibodies, wherein the nanosensor comprises a metal nanoparticle functionalised with one or more B-cell linear peptide epitopes derived from the spike (S) protein or nucleocapsid (N) protein of SARS-CoV-2. In some preferred embodiments, the metal nanoparticle comprises gold, silver, or a combination of gold and silver. The present invention also relates to various methods involving the use of the nanosensor.

Abstract: There is provided a method of evaluating risk of severe outcome of an infectious disease and/or an inflammatory disease in a patient, the method comprising: determining/measuring the number of one or more immune cells selected from the group consisting of VD2 T cells, CD8 T cells, and immature neutrophils in a sample obtained from the patient, wherein the patient has an enhanced risk of severe infectious disease and/or inflammatory disease outcome when: (i) the ratio of immature neutrophils to VD2 T cell is at least 2:1, and/or (ii) the ratio of immature neutrophils to CD8 T cell is at least 0.5:1. Also disclosed are method of treating a patient with a severe infectious disease and/or inflammatory disease and kit for use in methods thereof.

Abstract: Techniques described herein are directed to centralized identities for personalization of data presentation. A centralized identity is generated when similar user identifiers as stored across resources are determined. The centralized identity associates the user identifiers with each other and allows for user-related data from the multiple disparate resources to be queried and received. This user-related data can then be utilized to determine one or more personalizations to apply, and an application being used by the user may be modified to present the personalizations.

Abstract: There is provided a method of evaluating risk of severe outcome of an infectious disease and/or an inflammatory disease in a patient, the method comprising: determining/measuring the number of one or more immune cells selected from the group consisting of VD2 T cells, CD8 T cells, and immature neutrophils in a sample obtained from the patient, wherein the patient has an enhanced risk of severe infectious disease and/or inflammatory disease outcome when: (i) the ratio of immature neutrophils to VD2 T cell is at least 2:1, and/or (ii) the ratio of immature neutrophils to CD8 T cell is at least 0.5:1. Also disclosed are method of treating a patient with a severe infectious disease and/or inflammatory disease and kit for use in methods thereof.

Abstract: A first identifier associated with a brick-and-mortar store of a merchant and a second identifier associated with a designated physical area within the brick-and-mortar store can be accessed to generate a third identifier that is an identification code scannable by a computing device of a customer. The identification code can be received from the computing device of the customer and a menu of items associated with the merchant can be caused to be presented via the computing device of the customer. An indication of an order for an item of the menu of items associated with the third identifier is received from the computing device of the customer. Payment data associated with a payment instrument of the customer is received from the computing device of the customer, and payment for the order of the item is processed based at least in part on the payment data.

Abstract: The disclosure provides anti-DENV antibodies having a cross-reactivity to ZIKV and methods of making and using the same. The anti-DENV antibodies have uses that include treating or preventing ZIKV infection.

Abstract: Customer-device application sites (e.g., content presented via a progressive web applications) that are accessible via merchant-managed identifiers (e.g., identification codes) are described. An identification of a physical location associated with a merchant can be associated with an identification code. The identification code can be received from a computing device of a customer and, based at least in part on receiving the identification code, merchant data associated with the merchant can be caused to be presented via a web browser of the computing device of the customer. In an example, the merchant data can be presented via the web browser by a progressive web application downloaded onto the computing device of the customer via the web browser. An indication of an input received via the progressive web application can be received by the computing device of the customer and an operation based at least in part on the input can be performed.

Abstract: The disclosure provides anti-DENV antibodies having a cross-reactivity to ZIKV and methods of making and using the same. The anti-DENV antibodies have uses that include treating or preventing ZIKV infection.

Abstract: Provided herein are polypeptides, polynucleotides, expression vectors, infectious clones, virus particles and immunogenic compositions of recombinant alphaviruses which can be used as vaccines. Also provided are methods for eliciting an immune response against alphavirus infection using the immunogenic composition comprising the alphavirus mutants described herein.

Abstract: There is provided a method of identifying/characterising coronavirus infection in a human subject. In a specific embodiment the method comprising contacting a serum sample from the subject with an isolated host cell expressing a nucleotide that encodes for a codon optimised gene for SARS-CoV-2 spike protein (S protein); and detecting a binding of an antibody or an antigen-binding fragment thereof to said host cell using a flow cytometry and a labelled anti-human secondary antibody. Also disclosed is a method of identifying a subject having immunity for coronavirus, especially SARS-CoV-2 wherein a sample from a subject is incubated with said cell and is detected using the flow cytometry and the labelled anti-human secondary antibody. Nucleic acid encoding a SARS-CoV-2 S protein, viral vectors and host cells thereof are also disclosed.

Abstract: There is provided a method of evaluating risk of severe outcome of an infectious disease and/or an inflammatory disease in a patient, the method comprising: determining/measuring the number of one or more immune cells selected from the group consisting of VD2 T cells, CD8 T cells, and immature neutrophils in a sample obtained from the patient, wherein the patient has an enhanced risk of severe infectious disease and/or inflammatory disease outcome when: (i) the ratio of immature neutrophils to VD2 T cell is at least 2:1, and/or (ii) the ratio of immature neutrophils to CD8 T cell is at least 0.5:1. Also disclosed are method of treating a patient with a severe infectious disease and/or inflammatory disease and kit for use in methods thereof.

Abstract: Customer-device application sites (e.g., content presented via a progressive web applications) that are accessible via merchant-managed identifiers (e.g., identification codes) are described. An identification of a physical location associated with a merchant can be associated with an identification code. The identification code can be received from a computing device of a customer and, based at least in part on receiving the identification code, merchant data associated with the merchant can be caused to be presented via a web browser of the computing device of the customer. In an example, the merchant data can be presented via the web browser by a progressive web application downloaded onto the computing device of the customer via the web browser. An indication of an input received via the progressive web application can be received by the computing device of the customer and an operation based at least in part on the input can be performed.

Abstract: Provided herein are polypeptides, polynucleotides, expression vectors, infectious clones, virus particles and immunogenic compositions of recombinant alphaviruses which can be used as vaccines. Also provided are methods for eliciting an immune response against alphavirus infection using the immunogenic composition comprising the alphavirus mutants described herein.

Abstract: The present invention generally relates to polypeptides, polynucleotides, expression vectors, infectious clones, virus particles and immunogenic compositions of recombinant alphaviruses which can be used as vaccines. The present disclosure also relates to methods for eliciting an immune response against alphavirus infection using the immunogenic composition comprising the alphavirus mutants described herein.

Abstract: The disclosure provides anti-DENV antibodies having a cross-reactivity to ZIKV and methods of making and using the same. The anti-DENV antibodies have uses that include treating or preventing ZIKV infection.

Abstract: The present invention generally relates to polypeptides, polynucleotides, expression vectors, infectious clones, virus particles and immunogenic compositions of recombinant alphaviruses which can be used as vaccines. The present disclosure also relates to methods for eliciting an immune response against alphavirus infection using the immunogenic composition comprising the alphavirus mutants described herein.

Abstract: The invention generally relates to an imaging apparatus having a reinforced electrical signal transmission member, and method of use thereof. In certain aspects, the apparatus includes a hollow elongate body. The apparatus also includes an elongate electrical signal transmission member within the body. The elongate signal transmission member includes two or more outer jacket layers. There is an imaging device within the body and the signal transmission member is coupled to the imaging device.

Abstract: The invention generally relates to a rotational imaging apparatus with a monolithic shaft and methods of use thereof. In certain aspects, the apparatus includes a rotatable monolithic hollow elongate shaft. A rotatable elongate drive member is disposed within the shaft, and a rotatable elongate electrical signal transmission member is disposed within the drive member. The apparatus further includes an imaging device, and the shaft, the drive member and the signal transmission member are coupled to the imaging device.

Abstract: The invention generally relates to a rotational imaging apparatus and methods of use thereof. In certain aspects, the apparatus includes a hollow elongate body. The hollow body includes a proximal and a distal end, and the proximal end includes a connector. A rotatable drive member is disposed within the body, and a rotatable electrical signal transmission member is disposed within the drive cable. The drive member and the electrical signal transmission member are coupled to an imaging device within the body and a portion of the electrical signal transmission member is attached to the drive member distal to the connector.

Abstract: The present invention provides primers directed to conserved regions of the HA and NA genes of avian influenza virus subtypes H5 and H5N1, and provides a method for detecting avian influenza subtype H5 or H5N1.