Abstract: Devices, systems, methods, and kits configured to perform bioassays on live, intact tissue for precision bioanalysis in vitro are described. These approaches enable precision oncology by capturing determinants of therapeutic response to functional drug testing, such as viability and molecular signal generation, that can depend on tissue architecture, tumor heterogeneity, and the tumor microenvironment. The disclosure provides electrochemical aptamer sensors integrated into arrays of microfluidic traps for the analysis of micro-dissected tumors, as an example. The sensors are utilized with an example of periodic monitoring of cytochrome c (Cyt-C), a soluble cell death indicator, released by micro-dissected tumors.

Abstract: Devices, systems, and methods for trapping and manipulating portions of tissue are described. In an embodiment, the devices include an array of traps, wherein traps of the array of traps are shaped to trap a tissue sample; and a well is in registry and fluidic communication with a trap of the array of traps.

Abstract: Methods and microfluidic apparatus are disclosed for drug prediction. A microfluidic apparatus has (a) a plate, (b) a plurality of wells defined in the plate, (c) a plurality of closed microchannels defined in the plate, and (d) a sample platform defining a plurality of open microchannels, where the plurality of closed microchannels are each in communication with one of the plurality of wells and one of the plurality of open microchannels of the sample platform.

Abstract: Methods and microfluidic apparatus are disclosed for drug prediction. A microfluidic apparatus has (a) a plate, (b) a plurality of wells defined in the plate, (c) a plurality of closed microchannels defined in the plate, and (d) a sample platform defining a plurality of open microchannels, where the plurality of closed microchannels are each in communication with one of the plurality of wells and one of the plurality of open microchannels of the sample platform.