Abstract: The present invention relates to a computer-implemented method and corresponding apparatus for the accurate identification of subcellular structures from tomographic reconstructions. This invention allows to extract the 3D subcellular specificity directly from the tomographic phase-contrast data obtained from a typical flow cytometry configuration or any other experimental tomographic systems able to provide 3D quantitative phase-contrast tomograms. In particular, subcellular structures can be identified by using a novel computational segmentation method based on statistical inference from any 3D phase-contrast tomographic data.

Abstract: Disclosed a system for reconstructing an image of an object hidden by a flame. The system comprises a laser source emitting an infrared radiation and a lensless, off-axis interferometric arrangement that divides the infrared radiation into an object beam and a reference beam. The object beam is enlarged and then irradiates the object, that scatters it. The reference beam is enlarged and then interferes with the scattered object beam, so as to create a hologram. The system comprises an infrared detector which detects the hologram and a processor that reconstructs the image of the object by numerically processing the hologram.

Abstract: It is disclosed a system for reconstructing an image of an object hidden by a flame. The system comprises a laser source emitting an infrared radiation and a lensless, off-axis interferometric arrangement that divides the infrared radiation into an object beam and a reference beam. The object beam is enlarged and then irradiates the object, that scatters it. The reference beam is enlarged and then interferes with the scattered object beam, so as to create a hologram. The system comprises an infrared detector which detects the hologram and a processing unit which reconstructs the image of the object by numerically processing the hologram. The system therefore provides the object image based on digital holography at infrared wavelengths. Differently from known thermographic acquisition techniques, even if large portions of the object are hidden by the flame, the system allows to reconstruct an image of the whole object, with no blind areas.