Patents by Inventor Lisbeth Olsson
Lisbeth Olsson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9803254Abstract: The present invention relates to a method of preparing a strain of sugar fermenting Saccharomyces cerevisiae with capability to ferment xylose, wherein said method comprises different procedural steps. The method comprises mating a first sporulated Saccharomyces cerevisiae strain with a second Saccharomyces cerevisiae haploid strain. Thereafter, screening for mated cells is performed, growing such mated cells, and verifying that mated cells exhibit basic morphology by microscopic inspection. Thereafter, creation of a mixture of the mated cells is performed, subjecting the mixture to continuous chemostat cultivation and obtaining the sugar fermenting Saccharomyces cerevisiae cells with capability to ferment xylose is performed. The invention also comprises strains obtained by said method.Type: GrantFiled: October 15, 2014Date of Patent: October 31, 2017Assignee: Scandinavian Technology Group ABInventors: Nicklas Bonander, Lisbeth Olsson, Elia Tomas-Pejo
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Publication number: 20160244851Abstract: The present invention relates to a method of preparing a strain of sugar fermenting Saccharomyces cerevisiae with capability to ferment xylose, wherein said method comprises different procedural steps. The method comprises mating a first sporulated Saccharomyces cerevisiae strain with a second Saccharomyces cerevisiae haploid strain. Thereafter, screening for mated cells is performed, growing such mated cells, and verifying that mated cells exhibit basic morphology by microscopic inspection. Thereafter, creation of a mixture of the mated cells is performed, subjecting the mixture to continuous chemostat cultivation and obtaining the sugar fermenting Saccharomyces cerevisiae cells with capability to ferment xylose is performed. The invention also comprises strains obtained by said method.Type: ApplicationFiled: October 15, 2014Publication date: August 25, 2016Applicant: SCANDINAVIAN TECHNOLOGY GROUP ABInventors: Nicklas Bonander, Lisbeth Olsson, Elia Tomas-Pejo
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Patent number: 9115376Abstract: A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion and reduced xylitol production is described. The method comprises culturing the cells in a repetitive batch series in a medium at a xylose concentration of about 15-25 g/l and at a temperature of about 28-32° C., and thereafter lowering the xylose concentration in at least one step at a temperature of about 28-32° C. to obtain an increased selection pressure for improved xylose fermentation, improved ethanol production and reduced xylitol production, and continuing the culturing of the cells in said repetitive batch series. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.Type: GrantFiled: November 15, 2011Date of Patent: August 25, 2015Assignee: SCANDINAVIAN TECHNOLOGY GROUP ABInventors: Eva Albers, Lisbeth Olsson, Rakesh Koppram
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Patent number: 8962289Abstract: A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of Saccharomyces cerevisiae at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.Type: GrantFiled: November 15, 2011Date of Patent: February 24, 2015Assignee: Scandinavian Technology Group ABInventors: Eva Albers, Lisbeth Olsson, Rakesh Koppram
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Publication number: 20130295620Abstract: A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion and reduced xylitol production is described. The method comprises culturing the cells in a repetitive batch series in a medium at a xylose concentration of about 15-25 g/l and at a temperature of about 28-32° C., and thereafter lowering the xylose concentration in at least one step at a temperature of about 28-32° C. to obtain an increased selection pressure for improved xylose fermentation,improved ethanol production and reduced xylitol production, and continuing the culturing of the cells in said repetitive batch series. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.Type: ApplicationFiled: November 15, 2011Publication date: November 7, 2013Applicant: SCANDINAVIAN TECHNOLOGY GROUP ABInventors: Eva Albers, Lisbeth Olsson, Rakesh Koppram
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Publication number: 20130244247Abstract: A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of Saccharomyces cerevisiae at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.Type: ApplicationFiled: November 15, 2011Publication date: September 19, 2013Applicant: SCANDINAVIAN TECHNOLOGY GROUP ABInventors: Eva Albers, Lisbeth Olsson, Rakesh Koppram
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Patent number: 5720873Abstract: The invention relates to a method of floating calcium carbonate ore containing silicates as impurities. Floatation is performed in the presence of a quaternary ammonium compound and an alkylene oxide adduct of an amine compound. The silicate being concentrated in the float.Type: GrantFiled: March 1, 1996Date of Patent: February 24, 1998Assignee: Akzo Nobel NVInventors: Anders Klingberg, Lisbeth Olsson