Abstract: Provided are an enzyme immobilization carrier and a preparation method therefor, and an immobilized enzyme and a preparation method therefor. The enzyme immobilization carrier includes a resin ball matrix, an —N(CH2OOH)2 group linked on the resin ball matrix by means of a chemical bond, and a metal ion adsorbed on the —N(CH2OOH)2 group in a chelating manner through coordination. The resin ball matrix is an amino-type methacrylic resin and/or an epoxy-type methacrylic resin, and the resin ball matrix has a particle size of 200-700 ?m. Therefore, the problems in the related art that an enzyme immobilization carrier is relatively poor in enzyme compatibility and poor in immobilization effect, and an immobilized enzyme is relatively poor in activity, and relatively poor in stability during repeated use are solved.

Abstract: Provided are a catalyst loaded with a TEMPO-type compound, and a preparation method and use thereof. The catalyst loaded with the TEMPO-type compound uses a polymer resin as a carrier, and the polymer resin is a polystyrene resin containing a chloromethyl or bromomethyl, herein the content of the chloromethyl or bromomethyl in the polymer resin is 0.5-5.0 mmol g?1.

Abstract: Provided are an immobilized enzyme and an application thereof in continuous production. The immobilized enzyme is a Polyethyleneimine (PEI)-modified immobilized enzyme, and includes: an enzyme, which includes Amine Dehydrogenase (AmDH) and/or Formate Dehydrogenase (FDH); and a carrier, which is a cyanuric chloride-activated amino carrier. The problem in the prior art of poor performance of an immobilized enzyme is solved, the catalytic activity and reusability of the immobilized enzyme are improved, and the immobilized enzyme is suitable for the field of enzyme immobilization.

Abstract: Provided is an amination method for a polystyrene-type resin, and a method for immobilizing an enzyme using an aminated polystyrene-type resin. The amination method includes: in a solvent, a catalyst is used to catalyze a polystyrene-type resin and an enamine salt to perform a Friedel-Crafts alkylation reaction, to obtain an aminated polystyrene-type resin, herein the catalyst is a Lewis acid catalyst. By means of the Friedel-Crafts alkylation reaction, the enamine salt is grafted onto the polystyrene-type resin, and the polystyrene-type resin is aminated. The conditions of the Friedel-Crafts alkylation reaction are easy to control, the post-treatment process is simple, and it is only necessary to remove the catalyst and the unreacted enamine salt by washing. Therefore, the above amination method in the present application has few steps and is simple and easy to implement. Meanwhile, the amination method further avoids the use of a noble metal catalyst, thereby reducing production cost.

Abstract: The application provides an enzyme immobilization carrier and a preparation method thereof, an immobilized enzyme and a preparation method thereof. The above enzyme immobilization carrier is obtained by an amino modification or a cyanuric chloride modification of super-crosslinked polyvinyl alcohol. The use of the enzyme immobilization carrier provided by the application may effectively improve the stability and reusability of the immobilized enzyme. Moreover, due to the use of the form of enzyme covalent linkage, compared with an embedding method, the preparation method is no need for chemical reagent immersion and the like, it is beneficial to maintain the own activity of the enzyme, and promote the immobilized enzyme to have the better activity while the stability and reusability are kept.