Abstract: The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.

Abstract: The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.

Abstract: A server rack system includes a rack, a rack management module, a plurality of servers, a management network connection module and a power module. The rack management module is located in the rack and coupled to a management network line. The servers are located in the rack and respectively have a baseboard management controller (BMC), in which each of the BMCs is used for monitoring a working state of the server where the BMC resides. The power module provides a working voltage required by the rack management module, the management network connection module and the servers. The rack management module obtains overall power consumption information through the power module, and obtains power consumption information of each of the servers through the management network line, and is used for managing the servers according to the overall power consumption information and the power consumption information of each of the servers.

Abstract: A server rack system is provided, which includes a rack, a rack management module, a plurality of servers, and a management network connection module. The rack management module is located in the rack and coupled to a management network line (MNL). The servers are located in the rack. Each server includes a baseboard management controller (BMC), and each BMC is used to monitor a working status of the server where the BMC is located. The management network connection module is located in the rack and coupled to the MNL to be connected to the rack management module and the BMC through the MNL. The rack management module is used to allocate an Internet Protocol (IP) address to the BMC and manage each server through the BMC corresponding to each IP address.

Abstract: A server rack system includes a rack, a rack management module, a plurality of servers, a management network connection module and a power module. The rack management module is located in the rack and coupled to a management network line. The servers are located in the rack and respectively have a baseboard management controller (BMC), in which each of the BMCs is used for monitoring a working state of the server where the BMC resides. The power module provides a working voltage required by the rack management module, the management network connection module and the servers. The rack management module obtains overall power consumption information through the power module, and obtains power consumption information of each of the servers through the management network line, and is used for managing the servers according to the overall power consumption information and the power consumption information of each of the servers.

Abstract: A server rack system is provided, which includes a rack, a rack management module, a plurality of servers, and a management network connection module. The rack management module is located in the rack and coupled to a management network line (MNL). The servers are located in the rack. Each server includes a baseboard management controller (BMC), and each BMC is used to monitor a working status of the server where the BMC is located. The management network connection module is located in the rack and coupled to the MNL to be connected to the rack management module and the BMC through the MNL. The rack management module is used to allocate an Internet Protocol (IP) address to the BMC and manage each server through the BMC corresponding to each IP address.

Abstract: A server rack system includes a rack, a rack management module, a plurality of servers, a management network connection module, and an identification module. The rack management module is located in the rack and is coupled to a management network line. Each server is respectively plugged in the rack, and respectively has a baseboard management controller (BMC) used to monitor a working state of the server. The management network connection module is located in the rack and used to connect the rack management module and the BMCs through the management network line. The identification module is located in the rack, so as to enable the corresponding server to generate an identification code signal. The rack management module identifies a physical position of each server in the rack according to the identification code signal of each server and a corresponding relation record between the identification code signal and the physical position.

Abstract: This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic Clostridium perfringens (Cp) ?-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing the Pseudomonas fluorescens system, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cp is also disclosed.

Abstract: A method for reducing integral stress of a vertical probe with specific structure is disclosed. The vertical probe includes a probe tip, an insert part and a bent part. The bent part has a first circular arc and a second circular arc and the second circular arc is provided with a radius much greater than that of the first circular arc and the second circular arc is farther away from the probe tip and smoothly extends from the first circular arc. The integral stress of the probe during probing is effectively reduced to avoid overstress.

Abstract: The present invention relates to methods for the cryopreservation of transformed and non-transformed cells. Also provided by the subject invention are methods of recovering cells that have been cryopreserved. Cultures of cells that have been successfully recovered from cryopreservation are also provided.

Abstract: Stabilized enzyme compositions for use in nucleic acid amplification. Compositions are provided for the stabilization of one or more enzymes in a single stabilized formulation. Additional compositions incorporate a dried, stabilized enzyme mixture together with necessary cofactors and enzyme substrates in a single container for use upon rehydration. Also disclosed are methods for making and using stabilized enzyme compositions and kits for nucleic acid amplification incorporating the disclosed compositions.

Abstract: Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducability. Also described are useful methods for extending the dynamic range of transcription-based amplification systems.

Abstract: Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducability. Also described are useful methods for extending the dynamic range of transcription-based amplification systems.