Patents by Inventor LIUYU WANG

LIUYU WANG has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for asymmetrically preparing L-phosphinothricin by oxidation-reduction reaction through biological multi-enzyme coupling
    Patent number: 12215372
    Abstract: A method for asymmetrically preparing L-phosphinothricin by oxidation-reduction reaction through biological multienzyme coupling, where D,L-phosphinothricin as a raw material is catalyzed by an enzyme catalysis system to obtain L-phosphinothricin, wherein the enzyme catalysis system comprises a D-amino acid oxidase mutant for catalyzing D-phosphinothricin in D,L-phosphinothricin into 2-carbonyl-4-[hydroxy(methyl)phosphono] butyric acid and a transaminase for catalytic reduction of the 2-carbonyl-4-[hydroxy(methyl)phosphono] butyric acid into L-phosphinothricin; the D-amino acid oxidase mutant is obtained by mutation of D-amino acid oxidase in wild strain Rhodotorula taiwanensis at one of the following sites: (1) M213S-N54V-F58E; (2) M213S-N54V-F58E-D207A; (3) M213S-N54V-F58E-D207A-S60T.
    Type: Grant
    Filed: June 8, 2020
    Date of Patent: February 4, 2025
    Assignee: ZHEJIANG UNIVERSITY OF TECHNOLOGY
    Inventors: Yaping Xue, Feng Cheng, Liuyu Wang, Yuguo Zheng, Jianmiao Xu, Shuping Zou
  • METHOD FOR ASYMMETRICALLY PREPARING L-PHOSPHINOTHRICIN BY OXIDATION-REDUCTION REACTION THROUGH BIOLOGICAL MULTI-ENZYME COUPLING
    Publication number: 20220220516
    Abstract: A method for asymmetrically preparing L-phosphinothricin by oxidation-reduction reaction through biological multienzyme coupling, where D,L-phosphinothricin as a raw material is catalyzed by an enzyme catalysis system to obtain L-phosphinothricin, wherein the enzyme catalysis system comprises a D-amino acid oxidase mutant for catalyzing D-phosphinothricin in D,L-phosphinothricin into 2-carbonyl-4-[hydroxy(methyl)phosphono]butyric acid and a transaminase for catalytic reduction of the 2-carbonyl-4-[hydroxy(methyl) phosphono]butyric acid into L-phosphinothricin; the D-amino acid oxidase mutant is obtained by mutation of D-amino acid oxidase in wild strain Rhodotorula taiwanensis at one of the following four sites: (1) M213S; (2) M213S-N54V-F58E; (3) M213S-N54V-F58E-D207A; (4) M213S-N54V-F58E-D207A-S60T.
    Type: Application
    Filed: June 8, 2020
    Publication date: July 14, 2022
    Inventors: YAPING XUE, FENG CHENG, LIUYU WANG, YUGUO ZHENG, JIANMIAO XU, SHUPING ZOU