Patents by Inventor Liv Reinecke
Liv Reinecke has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10494654Abstract: A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.Type: GrantFiled: March 12, 2018Date of Patent: December 3, 2019Assignee: CARGILL, INCORPORATEDInventors: Katrin Grammann, Jan Wolter, Liv Reinecke, Steffen Schaffer, Eileen E. Spindler, Wendy K. Ribble, Brittany L. Robinson, Catherine B. Poor, Tanya Warnecke Lipscomb, Hans H. Liao, David A. Hogsett, Ronald J. Evans
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Patent number: 10174349Abstract: The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.Type: GrantFiled: April 28, 2009Date of Patent: January 8, 2019Assignee: EVONIK ROEHM GmbHInventors: Liv Reinecke, Steffen Schaffer, Achim Marx, Markus Poetter, Thomas Haas
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Publication number: 20180273990Abstract: A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.Type: ApplicationFiled: March 12, 2018Publication date: September 27, 2018Inventors: Katrin GRAMMANN, Jan WOLTER, Liv REINECKE, Steffen SCHAFFER, Eileen E. SPINDLER, Wendy K. RIBBLE, Brittany L. PRATHER, Catherine B. POOR, Tanya Warnecke LIPSCOMB, Hans H. LIAO, Dave A. HOGSETT, Ronald J. EVANS
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Publication number: 20180155749Abstract: There is provided a microbial cell for producing at least one acyl amino acid, wherein the cell is genetically modified to comprise: a first genetic mutation that increases the expression relative to a wild type cell of at least one lipase (EC 3.1.1) (E1) capable of hydrolysing at least one glyceride to at least one fatty acid wherein the glyceride is a triglyceride; and a second and a third genetic mutation that increases the expression relative to a wild type cell of: (i) an amino acid-N-acyl-transferase (EC 2.3.1) (E2), and (ii) an acyl-CoA synthetase (EC 6.2.1.3) (E3) respectively that enables the cell to convert the fatty acid to at least one acyl amino acid and wherein the cell has a reduced fatty acid degradation capacity.Type: ApplicationFiled: April 28, 2016Publication date: June 7, 2018Inventors: Katrin GRAMMANN, Hans-Georg HENNEMANN, Nicole DECKER, Nicole KIRCHNER, Liv REINECKE
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Publication number: 20180127791Abstract: The present invention relates to a microbial cell for producing at least one acyl amino acid, wherein the cell is genetically modified to comprise; a first genetic mutation that enables the cell to produce at least one acyl amino acid and; a second genetic mutation that enables the cell to decrease glutamate breakdown relative to the wild type cell.Type: ApplicationFiled: April 4, 2016Publication date: May 10, 2018Applicant: Evonik Degussa GmbHInventors: Steffen SCHAFFER, Katrin GRAMMANN, Liv REINECKE
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Patent number: 9944959Abstract: A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.Type: GrantFiled: September 2, 2015Date of Patent: April 17, 2018Assignee: CARGILL, INCORPORATEDInventors: Katrin Grammann, Jan Wolter, Liv Reinecke, Steffen Schaffer, Eileen C. Spindler, Wendy K. Ribble, Brittany L. Prather, Catherine B. Poor, Tanya Warnecke Lipscomb, Hans H. Liao, Dave A. Hogsett, Ronald J. Evans
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Publication number: 20170130248Abstract: The present invention relates to a cell for producing acyl glycinates wherein the cell is genetically modified to comprise at least a first genetic mutation that increases the expression relative to the wild type cell of an amino acid-N-acyl-transferase, at least a second genetic mutation that increases the expression relative to the wild type cell of an acyl-CoA synthetase, and at least a third genetic mutation that decreases the expression relative to the wild type cell of at least one enzyme selected from the group consisting of an enzyme of the glycine cleavage system, glycine hydroxymethyltransferase (GlyA) and threonine aldolase (LtaE).Type: ApplicationFiled: April 28, 2015Publication date: May 11, 2017Applicant: Evonik Degussa GmbHInventors: LIV REINECKE, STEFFEN SCHAFFER, KATRIN GRAMMANN, MAIK OLFERT, NICOLE DECKER, NILS ARTO, HANS-GEORG HENNEMANN
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Publication number: 20160272950Abstract: The present invention relates to a cell expressing an amino acid-N-acyl-transferase, which is preferably recombinant, and an acyl-CoA synthetase, which is preferably recombinant, wherein the cell has a reduced fatty acid degradation capacity, a method for producing acyl amino acids, comprising the step contacting an amino acid and an acyl CoA in the presence of an amino acid-N-acyl-transferase, which is preferably isolated and/or recombinant, wherein the amino acid-N-acyl-transferase is preferably a human amino acid-N-acyl-transferase, or culturing the cell and a reaction mixture comprising an amino acid-N-acyl-transferase, which is preferably isolated and/or recombinant, an acyl-CoA synthetase, which is preferably isolated and/or recombinant, an amino acid and either a fatty acid-CoA or a fatty acid and an acyl-CoA-synthase.Type: ApplicationFiled: August 25, 2014Publication date: September 22, 2016Applicant: EVONIK DEGUSSA GMBHInventors: Jasmin CORTHALS, Katrin GRAMMANN, Thomas HAAS, Maik OLFERT, Nicole POTGRAVE, Liv REINECKE, Steffen SCHAFFER, Jan WOLTER
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Publication number: 20160060663Abstract: A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.Type: ApplicationFiled: September 2, 2015Publication date: March 3, 2016Applicant: EVONIK INDUSTRIES AGInventors: Katrin GRAMMANN, Jan Wolter, Liv Reinecke, Steffen Schaffer, Eileen C. Spindler, Wendy K. Ribble, Brittany L. Prather, Catherine B. Poor, Tanya Warnecke Lipscomb, Hans H. Liao, Dave A. Hogsett, Ronald J. Evans
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Patent number: 8980594Abstract: The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.Type: GrantFiled: October 11, 2010Date of Patent: March 17, 2015Assignee: Evonik Roehm GmbHInventors: Liv Reinecke, Steffen Schaffer, Tim Koehler, Anja Thiessenhusen, Achim Marx, Markus Buchhaupt
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Publication number: 20120264182Abstract: The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.Type: ApplicationFiled: October 11, 2010Publication date: October 18, 2012Applicant: EVONIK ROEHM GMBHInventors: Liv Reinecke, Steffen Schaffer, Tim Koehler, Anja Thiessenhusen, Achim Marx, Markus Buchhaupt
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Publication number: 20110171702Abstract: The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.Type: ApplicationFiled: April 28, 2009Publication date: July 14, 2011Applicant: EVONIK ROEHM GMBHInventors: Liv Reinecke, Steffen Schaffer, Achim Marx, Markus Poetter, Thomas Haas