Abstract: The present disclosure provides a method and system for determining an optimal flight height of an unmanned aerial vehicle (UAV), an electronic device, and a medium. The method includes: obtaining a multi-band crop spectral image of an experimental area in a preset scenario; performing data processing on the multi-band crop spectral image, to obtain multi-spectral orthographies of a plurality of sample plots; calculating a ground resolution of the multi-spectral orthography of each sample plot, simulating and determining a multi-band crop spectral image of each sample plot at a different flight height by a nearest neighbor interpolation method based on the ground resolution of the multi-spectral orthography of each sample plot; and for the multi-band crop spectral image of each sample plot at the different flight height, determining an optimal flight height corresponding to each sample plot by a hypothetical test method.