Patents by Inventor Lizhi Chen

Lizhi Chen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for efficiently expressing pullulanase in bacillus subtilis and recombinant bacillus subtilis
    Patent number: 10975366
    Abstract: The present invention relates to the field of genetic engineering, particularly to a method for efficiently expressing pullulanase in Bacillus subtilis and recombinant Bacillus subtilis. said method includes steps of constructing modified Bacillus subtilis strain with deletion of alkaline protease gene and neutral protease gene, constructing expression vector including an optimized combination of promoter and signal peptide and pullulanase gene, and transforming said modified Bacillus subtilis strain with by said expression vector. A series of combinations of promoter and signal peptide are optimized to obtain the combination for efficiently expressing pullulanase, provide an industrial application basis.
    Type: Grant
    Filed: October 24, 2017
    Date of Patent: April 13, 2021
    Assignee: GUANGDONG VTR BIO-TECH CO., LTD
    Inventors: Yangyuan Li, Jianrong Wang, Ling Yang, Man Qu, Lizhi Chen, Jiale Huang
  • METHOD FOR EFFICIENTLY EXPRESSING PULLULANASE IN BACILLUS SUBTILIS AND RECOMBINANT BACILLUS SUBTILIS
    Publication number: 20200140839
    Abstract: The present invention relates to the field of genetic engineering, particularly to a method for efficiently expressing pullulanase in Bacillus subtilis and recombinant Bacillus subtilis. said method includes steps of constructing modified Bacillus subtilis strain with deletion of alkaline protease gene and neutral protease gene, constructing expression vector including an optimized combination of promoter and signal peptide and pullulanase gene, and transforming said modified Bacillus subtilis strain with by said expression vector. A series of combinations of promoter and signal peptide are optimized to obtain the combination for efficiently expressing pululanase, provide an industrial application basis.
    Type: Application
    Filed: October 24, 2017
    Publication date: May 7, 2020
    Inventors: Yangyuan Li, Jianrong Wang, Ling YANG, Man Qu, Lizhi Chen, Jiale Huang
  • Fast terminal box
    Patent number: D665363
    Type: Grant
    Filed: March 18, 2011
    Date of Patent: August 14, 2012
    Assignee: Renhe Photovoltaic Technology Co., Ltd.
    Inventors: Yueming Lin, Lijun Duan, Lizhi Chen