Abstract: The present disclosure is generally directed to anchoring primary nucleic acid probes in a polymer matrix, which are configured to hybridize to cellular nucleic acid, embedding in a polymer matrix, clearing cellular components including the cellular nucleic acid and imaging. The disclosure describes methods for imaging cellular RNA in an RNase insensitive method.

Abstract: The present disclosure is generally directed to methods for anchoring nucleic acid in a matrix and subsequently imaging nucleic acid targets (e.g. RNA transcript molecules) within tissue samples, for example, formalin-fixed paraffin-embedded (FFPE) tissue sections wherein the nucleic acid may be fragmented.

Abstract: An automatic positioning winder comprising a lower cover, an upper cover fixed to said lower cover, a winding device provided between said lower and upper covers, entering and outgoing line holes formed in the lateral walls of the lower and upper covers respectively; characterized in that: said winding device consists of a center pillar provided in center of an upper portion of the lower cover, a turning wheel sleeved on the center pillar, a brake component provided in the upper portion of the lower cover and a vulture spring fixed inside of the turning wheel; an annular groove having a flat bottom is formed on the bottom portion of the turning wheel; said annular groove includes a positioning device for forming a guiding groove inside the annular groove; the brake component has a hook portion moving relatively along said guiding groove as the turning wheel rotating.

Abstract: An automatic positioning winder comprising a lower cover, an upper cover fixed to said lower cover, a winding device provided between said lower and upper covers, entering and outgoing line holes formed in the lateral walls of the lower and upper covers respectively; characterized in that: said winding device consists of a centre pillar provided in center of an upper portion of the lower cover, a turning wheel sleeved on the centre pillar, a brake component provided in the upper portion of the lower cover and a vulture spring fixed inside of the turning wheel; an annular groove having a flat bottom is formed on the bottom portion of the turning wheel; said annular groove includes a positioning device for forming a guiding groove inside the annular groove; the brake component has a hook portion moving relatively along said guiding groove as the turning wheel rotating.

Abstract: An automatic positioning winder comprises a lower cover (1), an upper cover (2) fixedly connected with the lower cover (1), a winding device quipped between the lower and upper covers, entering and outgoing line holes (10, 11) formed in the lateral walls of the lower and upper covers respectively. The winding device consists of a centre pillar (34) provided in center of the upper part of the lower cover, a turning wheel (4) sleeved on the centre pillar, a brake component (5) provided in the upper part of the lower cover and a vulture spring (6) fixed inside of the turning wheel. An annular groove (7) is formed on the bottom part of the turning wheel and the bottom of the annular groove is flat. A positioning device (8) is provided in the annular groove for separating the annular groove. The annular groove is divided into guiding groove (9) by the positioning device. The brake component can be embedded into the guiding groove and moves relatively along the guiding groove (9) when the turning wheel (4) rotates.