Abstract: Disclosed are a molecular detection system and a detection method thereof. The molecular detection system includes a main control device and a plurality of molecular detection devices. The main control device communicates with each of the plurality of molecular detection devices. The main control device is configured to control each of the plurality of molecular detection devices to perform detection. The main control device includes a display module configured to display detection data of each of the plurality of molecular detection devices. The molecular detection devices may perform different types of detections on different types of samples, which greatly expands the application flexibility and the application scenarios while meeting the detection throughput.

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

Abstract: The disclosure relates to the field of molecular biological detection, specifically, to the field of detection of pathogens that cause respiratory tract infections. More specifically, the disclosure is capable of simultaneously detecting Influenza A virus, Influenza B virus, Rhinovirus, Respiratory adenovirus, Respiratory syncytial virus and Mycoplasma pneumoniae. Using the compositions of the disclosure, it is possible to detect six pathogens that cause respiratory tract infections at the same time and to identify specifically which pathogen(s) causes the infections. Also, it has a higher detectable rate than that of the existing composition, and is more accurate in detection.

Abstract: The disclosure belongs to the field of molecular biological detection, particularly belongs to the field of miRNA detection, and more particularly relates to one-step detection of miRNA. The disclosure provides a primer and probe design method for miRNA detection, a detection composition including a primer and probe designed by the primer and probe design method, and a detection kit including the detection composition. By using the primer and the probe designed by the method disclosed in the disclosure, miRNA detection may be carried out by adopting one-step method, and non-specific amplification is avoided; the method has a high sensitivity of 1000 copies/mL with good specificity, and the operation of the method is simpler and safer with more credible results.

Abstract: Provided is a composition for detecting novel coronavirus 2019-nCoV, influenza A virus, influenza B virus, respiratory adenovirus, respiratory syncytial virus, and Mycoplasma pneumoniae. Also provided are a kit containing the composition, a use of the composition, and a method for detecting and typing pathogens that cause a respiratory infection.

Abstract: Provided is a composition for detecting mutations of 2019 novel coronavirus mutation. Also provided are a kit containing the composition, the use of the composition, and a method for detecting mutations of 2019 novel coronavirus.

Abstract: Provided in the present invention is a composition capable of detecting and typing novel coronavirus 2019-nCoV, coronavirus 229E, coronavirus NL63, coronavirus OC43, coronavirus HKU1, coronavirus MERSr-CoV, and coronavirus SARSr-CoV. At the same time, further provided is a kit comprising the composition and a method for detecting and typing coronaviruses. The composition of the present invention in combination with a fluorescent probe method and a melting curve method can perform simultaneous detection and typing of seven coronaviruses in one tube.

Abstract: A composition for improving the detection performance of fluorescent quantitative PCR. The composition comprises bovine serum albumin, sorbitol, ammonium sulfate, formamide, tetramethylammonium chloride, and at least one of dithiothreitol and betaine. The present invention further relates to a qPCR reaction liquid containing the composition and a preparation method therefor. The composition can improve the sensitivity, specificity, and interference resistance of real-time fluorescent quantitative PCR.

Abstract: Provided is a composition for detecting a novel coronavirus, influenza A virus and influenza B virus; moreover, also provided are a kit including the composition, an application of the kit, and a method for detecting and typing viruses causing respiratory tract infection.

Abstract: Provided is a reagent combination for detecting a liver cancer. The reagent combination includes any one of the following detection reagents: 1) a detection reagent for detecting methylation level of at least one of the following methylation sites of the PAK1 gene: cg17202086, cg26996201, and cg18309286; 2) a detection reagent for detecting methylation level of at least one of the following methylation sites of the OTX1 gene: cg23229261 and cg10122865; and 3) a detection reagent for detecting methylation level of methylation site cg16657538 of the ZNF397OS gene. Further provided are use of the reagent combination, and a kit including the reagent combination. By using the reagent combination of the present invention, a liver cancer may be detected clinically in a tissue sample with fewer markers, and may be detected sensitively and specifically by detecting methylation sites, and the cost and time are saved.

Abstract: A composition for detecting SARS-CoV-2 is provided; moreover, a kit including the composition, the use of the kit, and a method for detecting SARS-CoV-2 are also provided.

Abstract: The present invention relates to the field of molecular biology detection, more particularly to a method for fluorescent quantitative PCR. The method includes: 1) mixing an upstream and downstream primer pair, a fluorescent probe, and a PCR amplification reagent; and 2) carrying out the fluorescent quantitative PCR, where the fluorescent probe has two quenching groups, in which a first quenching group is located at a 3? end and a second quenching group is labeled on a T base and is 10-15 nt apart from the first quenching group. Using the method for fluorescent quantitative PCR, background signals in the fluorescent quantitative PCR can be reduced; furthermore, the sensitivity of PCR can be improved, the occurrence of false negatives in detection can be reduced, and the amplification efficiency of the fluorescent quantitative PCR can also be improved.

Abstract: Provided is a composition for detecting multiple encephalitis/meningitis/respiratory pathogens. The composition includes primer sequences for the pathogens as shown in SEQ ID NOs: 1-7, 9-23 and 25-32, in which SEQ ID NOs: 1-7 and 9-16 carry fluorescent reporter groups. In addition, the present invention further provides a use of the foregoing composition in the preparation of a kit, and a related kit and a method for using the same.

Abstract: The present invention relates to the field of viral nucleic acid detection. In particular, the present invention provides a pretreatment method for viral nucleic acid detection. The method includes mixing a pretreatment solution containing a sample with a nucleic acid releasing agent and a qPCR amplification reagent, wherein the pretreatment solution includes Tris-HCl, EDTA-2Na, sodium chloride, a ribonuclease (RNase) inhibitor, and an antibiotic; and the pretreatment solution has a pH of 6.5-8.0.

Abstract: A nucleic acid release agent, a PCR amplification method and a PCR amplification kit are provided. The nucleic acid release agent includes Tris-HCl, sodium chloride, potassium chloride, tween 20, Triton X-100, ethyl phenyl polyethylene glycol and a strong base; wherein the molar concentration of Tris-HCl is 0.5 mM to 500 mM, the molar concentration of sodium chloride is 20 mM to 500 mM, the volume percentage of Tween 20 is 0.1% to 2%, the volume percentage of Triton X-100 is 0.1% to 3%, the volume percentage of ethyl phenyl polyethylene glycol is 0.1% to 3%, the mass concentration of potassium chloride is 5 mg/mL to 8 mg/mL and the mass concentration of the strong base is 2 mg/mL to 50 mg/mL.

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

Abstract: A rapid amplification method for a nucleic acid of hepatitis B virus, comprises the following steps: mixing a sample containing hepatitis B virus with a nucleic acid releasing agent, and adding a PCR premix to obtain a reaction solution, the PCR premix comprising deoxyribonucleoside triphosphate, an upstream primer as shown in the sequence SEQ ID NO: 1, a downstream primer as shown in the sequence SEQ ID NO: 2, a DNA polymerase and an amplification buffer; placing the reaction solution in a PCR reaction tube so that the reaction solution is in a form of a thin film with a thickness of 0.1 mm or less; performing PCR amplification under the following reaction condition: pre-denaturation at 90 to 100° C. for 10 s to 600 s, denaturation at 90 to 100° C. for 0 to 1 s, and annealing and extending at 50-65° C. for 0 to 1 s.

Abstract: The present application provides a magnetic bead-based nucleic acid extraction apparatus including a well plate, a magnetic attraction mechanism, a position moving mechanism, and an oscillation mechanism, a supporting base, and a fixing base disposed on the supporting base. The magnetic attraction mechanism includes a magnetic bar fixing base and a magnetic bar movably disposed on the magnetic bar fixing base. The position moving mechanism includes a ball screw and a driving motor; two ends of the ball screw are respectively connected to the magnetic bar fixing base and the driving motor. The oscillation mechanism includes a carrier and a main oscillation unit connected to the carrier. The main oscillation unit includes a motor spindle, an eccentric column, and a connecting base.

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.