Abstract: Is disclosed a device for manipulating a particle immersed in a fluid, comprising a probe having a first end, a second end and a longitudinal axis. The probe receives a radiation from a light source and emits the radiation by means of the second end. The probe comprises: an optical guide structure suitable for receiving the radiation. The optical guide structure is configured so that: at the second end, the radiation has an optical intensity distribution with an intensity maximum placed at a non-zero distance from the longitudinal axis of the probe; and in the region of the intensity maximum, the radiation is reflected at the interface between the second end and the fluid and is emitted by the second end so that it converges in a convergence point, thus creating an equilibrium point. The probe further comprises perturbation optical means for perturbing the equilibrium point.