Abstract: A system and method for improved capture of neurodata and other biometric information includes an array of sensing positions integrated into a textile and configured to collect signals associated with a subject and a processor in communication with the array of sensing positions, where the processor is configured to identify biometric information within the signals. The array of sensing positions being distributed over an area of the textile to provide a “swarm sensing” capability to collect brain waves (or other related data) without discrete sensors on the head.

Abstract: The present invention is directed to methods and materials for RNA-mediated gene assembly from oligonucleotide sequences. In some embodiments, the oligonucleotides used for gene assembly are provided in an array format. An RNA polymerase promoter is appended to surface-bound oligonucleotides and a plurality of RNA copies of each oligonucleotide are then produced with an RNA polymerase. These RNA molecules self-assemble into a desired full-length RNA transcript by hybridization and ligation. The resulting RNA transcript may then be converted into double stand DNA useful in a variety of applications including protein expression.

Abstract: A cooking appliance includes a cooktop having at least one heating element for heating a cookware member on or to be placed on the cooktop, the at least one heating element being adjustable between a working-power level wherein the at least one heating element is energized to generate heat and a zero-power level wherein the at least one heating element is not energized. The cooking appliance further includes a temperature sensor configured to detect a temperature or rate of temperature change of a cooking element. The cooking appliance further includes a control device configured to adjust the at least one heating element from the working-power level to the zero-power level based on the temperature or rate of temperature change of the cooking element. The at least one heating element will remain at the zero-power level until a user intervenes to re-energize the at least one heating element.

Abstract: Systems for monitoring metabolites may include a sample collection system and a urinalysis system of or for a toilet. The sample collection system may be automated to collect sample urine from a toilet or toilet area, transport the sample to the urinalysis system, and/or the sample urine for analysis at a later time or after analysis by the urinalysis system. The urinalysis system may be automated by proactively providing test material on which a subject may provide a urine sample. Once the urine sample has been provided the urinalysis system may provide the test material to an analyzer for analysis. Results of the analysis of the test material and sample urine may be provided to a mobile device of the subject and/or a remote server. The urinalysis system may provide a urine sample to a mass spectrometry unit for analysis.

Abstract: Described herein are RNA arrays, and compositions and methods for generating RNA arrays, particularly high density RNA arrays. The disclosed methods for generating RNA arrays utilize template DNA arrays and RNA polymerase to generate RNA arrays. In some embodiments, the disclosed methods use an RNA polymerase and modified ribonucleosides to generate modified RNA arrays for various applications, e.g. RNA arrays having higher nuclease resistance, more conformationally stable RNA arrays, and higher binding affinity RNA aptamer arrays. In some embodiments, the disclosed methods are used to generate RNA bead arrays.

Abstract: A cooking appliance includes a cooktop having at least one heating element for heating a cookware member on or to be placed on the cooktop, the at least one heating element being adjustable between a working-power level wherein the at least one heating element is energized to generate heat and a zero-power level wherein the at least one heating element is not energized. The cooking appliance further includes a temperature sensor configured to detect a temperature or rate of temperature change of a cooking element. The cooking appliance further includes a control device configured to adjust the at least one heating element from the working-power level to the zero-power level based on the temperature or rate of temperature change of the cooking element. The at least one heating element will remain at the zero-power level until a user intervenes to re-energize the at least one heating element.

Abstract: A switch for operating a heating element of a cooking appliance includes a first contact electrically connected with the heating element, a second contact electrically connected with a power source. A bimetal strip is configured to electrically connect and disconnect the first and second contacts. The switch further includes a rotatable cam member having a cam surface for operative engagement with a cam follower. The cam surface has a profile dimension that is at least partially variable about the rotational axis of the cam member and is configured to cause displacement of the cam follower as a function of its rotational orientation to thereby adjust an operating temperature of the heating element. The cam surface is configured such that the operating temperature can be adjusted up to but not beyond a predetermined maximum temperature via rotation of the cam member. Circuits incorporating such a switch also are disclosed.

Abstract: A cooking appliance includes a cooktop having at least one heating element for heating a cookware member on or to be placed on the cooktop, the at least one heating element being adjustable between a working-power level wherein the at least one heating element is energized to generate heat and a zero-power level wherein the at least one heating element is not energized. The cooking appliance further includes a temperature sensor configured to detect a temperature or rate of temperature change of a cooking element. The cooking appliance further includes a control device configured to adjust the at least one heating element from the working-power level to the zero-power level based on the temperature or rate of temperature change of the cooking element. The at least one heating element will remain at the zero-power level until a user intervenes to re-energize the at least one heating element.

Abstract: The present invention is directed to methods and materials for RNA-mediated gene assembly from oligonucleotide sequences. In some embodiments, the oligonucleotides used for gene assembly are provided in an array format. An RNA polymerase promoter is appended to surface-bound oligonucleotides and a plurality of RNA copies of each oligonucleotide are then produced with an RNA polymerase. These RNA molecules self-assemble into a desired full-length RNA transcript by hybridization and ligation. The resulting RNA transcript may then be converted into double stand DNA useful in a variety of applications including protein expression.

Abstract: Described herein are RNA arrays, and compositions and methods for generating RNA arrays, particularly high density RNA arrays. The disclosed methods for generating RNA arrays utilize template DNA arrays and RNA polymerase to generate RNA arrays. In some embodiments, the disclosed methods use an RNA polymerase and modified ribonucleosides to generate modified RNA arrays for various applications, e.g. RNA arrays having higher nuclease resistance, more conformationally stable RNA arrays, and higher binding affinity RNA aptamer arrays. In some embodiments, the disclosed methods are used to generate RNA bead arrays.

Abstract: A household appliance including a heating element at an upper surface of the appliance, and a variable height downdraft vent assembly movable relative to the upper surface. The vent assembly includes a duct defining a flow passage between an upper end and a lower end thereof. The duct is movable in a vertical direction relative to the upper surface of the appliance. A drive assembly is provided for engaging the duct to position the upper end of the duct at a user-selected variable height relative to the upper surface of the appliance.

Abstract: The present invention is directed to methods and materials for RNA-mediated gene assembly from oligonucleotide sequences. In some embodiments, the oligonucleotides used for gene assembly are provided in an array format. An RNA polymerase promoter is appended to surface-bound oligonucleotides and a plurality of RNA copies of each oligonucleotide are then produced with an RNA polymerase. These RNA molecules self-assemble into a desired full-length RNA transcript by hybridization and ligation. The resulting RNA transcript may then be converted into double strand DNA useful in a variety of applications including protein expression.

Abstract: A switch for operating a heating element of a cooking appliance includes a first contact electrically connected with the heating element, a second contact electrically connected with a power source. A bimetal strip is configured to electrically connect and disconnect the first and second contacts. The switch further includes a rotatable cam member having a cam surface for operative engagement with a cam follower. The cam surface has a profile dimension that is at least partially variable about the rotational axis of the cam member and is configured to cause displacement of the cam follower as a function of its rotational orientation to thereby adjust an operating temperature of the heating element. The cam surface is configured such that the operating temperature can be adjusted up to but not beyond a predetermined maximum temperature via rotation of the cam member. Circuits incorporating such a switch also are disclosed.

Abstract: A cooking appliance includes a cooktop having at least one heating element for heating a cookware member on or to be placed on the cooktop, the at least one heating element being adjustable between a working-power level wherein the at least one heating element is energized to generate heat and a zero-power level wherein the at least one heating element is not energized. The cooking appliance further includes a temperature sensor configured to detect a temperature or rate of temperature change of a cooking element. The cooking appliance further includes a control device configured to adjust the at least one heating element from the working-power level to the zero-power level based on the temperature or rate of temperature change of the cooking element. The at least one heating element will remain at the zero-power level until a user intervenes to re-energize the at least one heating element.

Abstract: Described herein are RNA arrays, and compositions and methods for generating RNA arrays, particularly high density RNA arrays. The disclosed methods for generating RNA arrays utilize template DNA arrays and RNA polymerase to generate RNA arrays. In some embodiments, the disclosed methods use an RNA polymerase and modified ribonucleosides to generate modified RNA arrays for various applications, e.g. RNA arrays having higher nuclease resistance, more conformationally stable RNA arrays, and higher binding affinity RNA aptamer arrays. In some embodiments, the disclosed methods are used to generate RNA bead arrays.

Abstract: The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5? nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.

Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic kit facilitating relative quantification and providing tangible evidence for the existence of specific functional groups. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules, such as biological samples. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. Advantageously, the labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.

Abstract: This invention provides methods, devices and device components for preparing ions from liquid samples containing chemical species and methods and devices for analyzing chemical species in liquid samples. The present invention provides an ion source for generating analyte ions having a selected charge state distribution, such as a reduced charged state distribution, that may be effectively interfaced with a variety of charged particle analyzers, including virtually any type of mass spectrometer.

Abstract: The present invention provides methods, devices and device components for detecting, sensing and analyzing molecules. Detectors of the present invention provide good detection sensitivity over a wide range of molecular masses ranging from a few Daltons up to 10s of megadaltons, which does not decrease as function of molecular mass. Sensors and analyzers of the present invention detect emission from an array of resonators to determine the molecular masses and/or electric charges of molecules which impact or contact an external surface of a membrane that is used to mount and excite the resonators in the array. Resonators in the array are excited via piezoelectric and/or magnetic excitation of the mounting membrane and, optionally, grid electrodes are used in certain configurations for electrically biasing for the resonator array, and for amplification or suppression of emission from the resonators so as to provide detection and mass/electric charge analysis with good sensitivity and resolution.

Abstract: An assay for analysis of a nucleic acid sample is provided. In one embodiment, the assay includes: a) employing an RNA sample to obtain a DNA probe; b) contacting the DNA probe with a substrate containing a surface-immobilized RNA oligonucleotide to produce a surface-immobilized RNA/DNA duplex; and c) detecting RNAseH-dependent cleavage of the surface-immobilized RNA oligonucleotide in the RNA/DNA duplex.