Abstract: Compositions include CRISPR RNAs, guide RNAs, and nucleic acid molecules encoding the same. Vectors and host cells comprising the nucleic acid molecules are also provided. Further provided are RNA-guided nuclease (RGN) systems for cleaving a mutHTT allele, wherein the RGN system comprises an RNA-guided nuclease and a guide RNA. The compositions find use in cleaving or modifying a mutHTT allele, and/or modifying the expression of a mutHTT allele. The compositions are additionally useful for treating Huntington's disease (HD), particularly in an allele-specific manner.

Abstract: Disclosed herein are novel banding systems that can be used to provide containment of a filling material during treatment of a tooth. In some embodiments, the systems can compensate for missing walls in a tooth to provide containment of a filling material during reconstruction of the tooth. Some embodiments include a system that includes a band. The band includes a plurality of sections and the plurality of sections includes a plurality of support sections and a plurality of elastic sections. In such embodiments, a pair of the plurality of support sections is connected by at least one elastic section of the plurality of elastic sections. And, in such embodiments, the band is configured to fit around side surfaces of a tooth.