Abstract: A microscope: includes a beam source producing a laser beam, an objective lens, and first and second scanning mirrors operating along perpendicular axes to reflect the laser beam to scan a specimen through the objective lens. A first pair of cylindrical lenses is positioned between the objective lens and the first scanning mirror at points along the straight line, with a focal point aligned with the first scanning mirror, to receive the reflected laser beam and provide it to the objective lens. A second pair of cylindrical lenses is positioned between the objective lens and the second scanning mirror along the straight line, with a focal point aligned with the second scanning mirror, to receive the reflected laser beam and provide it to the objective lens.

Abstract: A method of performing imaging includes operating a light sheet projection module in a first state during a first measurement process and using a first primary objective for illumination of a specimen using a light sheet and detection of a first fluorescent emission. The method also includes operating the light sheet projection module in a second state during a second measurement process and using a second primary objective for illumination of the specimen using the light sheet and detection of a second fluorescent emission.

Abstract: A microscope is provided, comprising: a beam source that produces a laser beam; first and second scanning mirrors configured to operate along respective first and second perpendicular axes to reflect the laser beam from the beam source to scan a specimen via an objective lens; a first pair of cylindrical lenses, positioned between the objective lens and the first scanning mirror, at points along a straight line, with a focal point aligned with the first scanning mirror, that receive the reflected laser beam from the first scanning mirror and provide the laser beam to the objective lens; and a second pair of cylindrical lenses, positioned between the objective lens and the second scanning mirror, at points along the line, with a focal point aligned with the second scanning mirror, that receive the reflected laser beam from the second scanning mirror and provide the laser beam to the objective lens.

Abstract: A method of performing imaging includes operating a light sheet projection module in a first state during a first measurement process and using a first primary objective for illumination of a specimen using a light sheet and detection of a first fluorescent emission. The method also includes operating the light sheet projection module in a second state during a second measurement process and using a second primary objective for illumination of the specimen using the light sheet and detection of a second fluorescent emission.